List Price Quantity
2150-0150
TBC

Collagen IV

  • Product Code
    2150-0150
  • Description
    Native Human Collagen IV
  • Product Type
    Purified Protein
  • Format
    Purified
  • Applications
    E
  • Pack Size
    0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

Collagen IV Product Summary

Product Information

Human collagen type IV from placenta.

Human collagen type I-III, V, VI (<5%). Non collagen proteins (<0.5%).

Target Species

Human

Product Form

Purified protein - liquid

Preparation

Salt and alcohol precipitations, DEAE chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement, ability to form microfibrils and by reaction with anti-collagen type specific monoclonal antibodies.

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

Purity

SDS PAGE: >95%

Approx. Protein Concentrations

Total protein concentration 1.0mg/ml.

Molecular Weight

340 kDa

Buffer Solution

Phosphate buffered saline

Storage

Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Collagen IV Additional Formats

Collagen IV Related Reagents

Collagen IV More Images

Collagen IV Application Notes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
Application NameYesNoNot DeterminedSuggested Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Collagen IV References

References

1. Glanville, R.W. et al. (1979) Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains
Eur. J. Biochem. 95: 383-389.
2. Sage, H. et al . (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagen
Biochemistry. 18: 3815-3822.
3. Klasson, S.C. et al . (1986) The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta.
Coll. Relat. Res. 6: 397-402.
4. Bornstein, P. (1980) Structurally distinct collagen types
Ann. Rev. Biochem. V. 49: 957-1003.

Collagen IV More Information

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