List Price Quantity
2150-0150
TBC

Collagen IV

  • Product Code
    2150-0150
  • Description
    Native Human Collagen IV
  • Product Type
    Purified Protein
  • Format
    Purified
  • Applications
    E
  • Pack Size
    0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

Product Information

Human collagen type IV from placenta.

Human collagen type I-III, V, VI (<5%). Non collagen proteins (<0.5%).

Target Species

Human

Product Form

Purified protein - liquid

Preparation

Salt and alcohol precipitations, DEAE chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement, ability to form microfibrils and by reaction with anti-collagen type specific monoclonal antibodies.

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

Purity

SDS PAGE: >95%

Approx. Protein Concentrations

Total protein concentration 1.0mg/ml.

Molecular Weight

340 kDa

Buffer Solution

Phosphate buffered saline

Storage

Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Application

Application NameYesNoNot DeterminedSuggested Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References

1. Glanville, R.W. et al. (1979) Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains
Eur. J. Biochem. 95: 383-389.
2. Sage, H. et al . (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagen
Biochemistry. 18: 3815-3822.
3. Klasson, S.C. et al . (1986) The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta.
Coll. Relat. Res. 6: 397-402.
4. Bornstein, P. (1980) Structurally distinct collagen types
Ann. Rev. Biochem. V. 49: 957-1003.
15% OFF

Contact Information

-    Contact Your Local Office

-    Worldwide Office Locations


Online Payment Methods:

-    Invoice / Purchase Order

-    Credit Card (Maestro, VISA,
     MasterCard & VISA Electron)