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GAPDH antibody

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Anti GAPDH (C-Terminal) Antibody thumbnail image 1 Anti GAPDH (C-Terminal) Antibody thumbnail image 2 Anti GAPDH (C-Terminal) Antibody thumbnail image 3
Anti GAPDH (C-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysate from HeLa human epithelial adenocarcinoma cells probed with Rabbit anti Human GAPDH (AHP996) at 0.5(A), 1(B) and 2(C) ug/ml
Anti GAPDH (C-Terminal) Antibody gallery image 2
Immunocytochemical staining of HeLa cells with Rabbit anti Human GAPDH (AHP996)
Anti GAPDH (C-Terminal) Antibody gallery image 3
Published customer image:
Rabbit anti GAPDH (C-Terminal) antibody (AHP996) used as a loading control for murine liver lysate samples in western blotting.
Image caption:
Expression of LXR, SREBP1-c, FASN, and PPARγ. Western blot analysis of LXR, SREBP1-c, FASN, and PPARγ in liver samples among diverse groups.

From: Juan Li, Qian Hu, Dai Xiao-yu, Lv Zhu, Yi-fan Miao, Hong-xin Kang, Xian-lin Zhao, Jia-qi Yao, Dan Long, Wen-fu Tang, Mei-hua Wan,
"Effect of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLD",
Evidence-Based Complementary and Alternative Medicine, vol. 2020, Article ID 6697638, 15 pages, 2020.
10.1155/2020/6697638
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rabbit anti GAPDH (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
GAPDH
Region
(C-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP996
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti GAPDH (C-Terminal) antibody recognizes an epitope within the C-Terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes.

GAPDH is a homotetramer molecule consisting of four 36kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalysing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate.

GAPDH has a role as a multifunctional protein and is involved in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to ß-amyloid precursor protein (Alzheimers disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdons disease) and the androgen receptor (spinobulbar muscular atrophy), indicates roles for GAPDH in the pathogenesis of neurodegenerative diseases (De Santo et al. 2010).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human GAPDH (CT) were raised by repeated immunisation of rabbits with highly purified antigen.

Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 16 amino acid peptide located near human GAPDH carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP996 detects a band of approximately 37kDa in HeLa cell lysates.

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References for GAPDH antibody

  1. De Santo, C. et al. (2010) Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A.
    Nat Immunol. 11 (11): 1039-46.
  2. Weeke-Klimp, A. et al. (2010) Epicardium-derived cells enhance proliferation, cellular maturation and alignment of cardiomyocytes.
    J Mol Cell Cardiol. 49: 606-16.
  3. Deacon, K. and Knox, A.J. (2010) Endothelin-1 (ET-1) increases the expression of remodeling genes in vascular smooth muscle through linked calcium and cAMP pathways: role of a phospholipase A(2)(cPLA(2))/cyclooxygenase-2 (COX-2)/prostacyclin receptor-dependent autocrine loop.
    J Biol Chem. 285: 25913-27.
  4. Li, J. et al. (2020) Effect of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLD
    Evidence-Based Complementary and Alternative Medicine. 2020: 1-15.

Further Reading

  1. Mazzola, J.L. & Sirover, M.A. (2001) Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts.
    J Neurochem. 76 (2): 442-9.
  2. Sirover, M.A. (2005) New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells.
    J Cell Biochem. 95 (1): 45-52.

Western Blotting

Synonyms
GLYCERALDEHYDE-3-PHOSPHATE Dehydrogenase
RRID
AB_609780
UniProt
P04406
Entrez Gene
GAPDH
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
GO:0050821 protein stabilization
GO:0035605 peptidyl-cysteine S-nitrosylase activity
GO:0035606 peptidyl-cysteine S-trans-nitrosylation
GO:0048471 perinuclear region of cytoplasm
GO:0051287 NAD binding
GO:0051402 neuron apoptosis

AHP996

151375 157316 159455

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