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IgG antibody

Goat anti Human IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

Product Code Applications Pack Size List Price Your Price Qty
5172-2504
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E WB 2 mg loader
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loader

Goat anti Human IgG antibody is specific for Human IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum.

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
Reconstitution
Reconstitute with 1ml deionised water. For extended storage aliquot and freeze.
Antiserum Preparation
Goat anti Human IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography with human IgG coupled to agarose beads followed by solid phase adsorbtion to remove unwanted cross reactivity.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Gentamicin sulphate
1%Bovine Serum Albumin
Immunogen
Human IgG, whole molecule.
Approx. Protein Concentrations
Ig concentration 2.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/25,000 1/100,000
Immunohistology - Frozen 1/1,000 1/5,000
Western Blotting 1/2,000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for IgG antibody

  1. Golchin, M. et al. (2023) Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen.
    BMC Biotechnol. 23 (1): 46.

RRID
AB_619882
UniProt
P01859
P01860
P01861
P01834
P01857
P0CG04
Entrez Gene
IGKC
IGHG1
IGHG2
IGHG3
IGHG4
IGLV2-14
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response

5172-2504

148712 154269 155639 160652

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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