J Chain antibody | Mc19-9
Mouse anti Human J chain antibody, clone Mc19-9 (MCA693) used to label J chain in human duodenum by immunofluorescence.
Image caption:
ecretory immunoglobulin A (SIgA) is reduced in splenectomized patients. (A) Cryostat sections of the duodenum were stained in red with phalloidin (a toxin that binds and stains filamentous actin) and in green with antibodies against either IgA (left panels), secretory component (SC, middle panels), or J-chain (right panels). Biopsies of one healthy donor (HD) and two representative splenectomized patients (pt.1 and pt.2) are shown. A reduction of IgA+ plasma cells, SIgA, SC, and J chain is observed after removal of the spleen.
From: Carsetti R, Di Sabatino A, Rosado MM, Cascioli S, Piano Mortari E, Milito C, Grimsholm O, Aranburu A, Giorda E, Tinozzi FP, Pulvirenti F, Donato G, Morini F, Bagolan P, Corazza GR, Quinti I.
Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen Axis.
Front Immunol. 2020 Jan 8;10:2937.
doi: 10.3389/fimmu.2019.02937.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human J Chain
- Product Type
- Monoclonal Antibody
- Clone
- Mc19-9
- Isotype
- IgG1
- Specificity
- J Chain
Antibody Quality and Performance Guarantee
| Mouse anti Human J chain antibody, clone Mc19-9 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. Mouse anti Human J chain antibody, clone Mc19-9 does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques Mouse anti Human J chain antibody, clone Mc19-9 detects J chain in IgM only under reducing conditions. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human J Chain.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml | |
| Immunohistology - Frozen | |
1/200 | |
| Immunoprecipitation | |
20ug/ml | |
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for J Chain antibody
-
Arnold, J.N. et al. (2005) Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin.
J Biol Chem. 280 (32): 29080-7. -
Almogren, A. et al. (2006) Purification, properties and extended solution structure of the complex formed between human immunoglobulin A1 and human serum albumin by scattering and ultracentrifugation.
J Mol Biol. 356 (2): 413-31. -
Carsetti, R. et al. (2020) Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen Axis.
Front Immunol. 10: 2937.
- RRID
- AB_2121623
MCA693
150744
157642
164671
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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