Thyroglobulin

Sandwich ELISA analysis of human thyroglobulin using Mouse anti Human thyroglobulin , clone 5F9 (<a rel="nofollow" href='http://www.abdserotec.com/human-thyroglobulin-antibody-5f9-bgn-6e10-8900-0700.html'target="_blank">8900-0700</a>) as a capture reagent and biotinylated Mouse anti Human thyroglobulin, clone 5E6 (<a rel="nofollow" href='http://www.abdserotec.com/human-thyroglobulin-antibody-5e6-bgn-6d7-8900-0730.html'target="_blank">8900-0730</a>) as a detection reagent with purified human thyroglobulin as antigen (PHP236) for production of a standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (<a rel="nofollow" href='http://www.bio-rad.com/en-us/sku/168-1135-imark-microplate-absorbance-reader'target="_blank">Bio-Rad Catalog 168-1135</a>). Serum samples (green and blue) and plasma samples (orange and red) are shown

Sandwich ELISA analysis of human thyroglobulin using Mouse anti Human thyroglobulin , clone 5F9 (8900-0700) as a capture reagent and biotinylated Mouse anti Human thyroglobulin, clone 5E6 (8900-0730) as a detection reagent with purified human thyroglobulin as antigen (PHP236) for production of a standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples (green and blue) and plasma samples (orange and red) are shown

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  • Sandwich ELISA analysis of human thyroglobulin using Mouse anti Human thyroglobulin , clone 5F9 (<a rel="nofollow" href='http://www.abdserotec.com/human-thyroglobulin-antibody-5f9-bgn-6e10-8900-0700.html'target="_blank">8900-0700</a>) as a capture reagent and biotinylated Mouse anti Human thyroglobulin, clone 5E6 (<a rel="nofollow" href='http://www.abdserotec.com/human-thyroglobulin-antibody-5e6-bgn-6d7-8900-0730.html'target="_blank">8900-0730</a>) as a detection reagent with purified human thyroglobulin as antigen (PHP236) for production of a standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (<a rel="nofollow" href='http://www.bio-rad.com/en-us/sku/168-1135-imark-microplate-absorbance-reader'target="_blank">Bio-Rad Catalog 168-1135</a>). Serum samples (green and blue) and plasma samples (orange and red) are shown
  • Native Human Thyroglobulin
(Rated 0.0/5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Format Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP236E1 mg
    PHP236
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Target Species
      Human
    • Product Form
      Purified protein from human thyroid glands - liquid
    • Preservative Stabilisers
      None present.
    • Purity
      SDS PAGE: >98%, ultra-pure, IgG low
    • Approx. Protein Concentrations
      1.0 mg/ml
    • Molecular Weight
      660 kDa, confirmed by chromatography and electrophoresis. Under certain conditions the subunit molecular weight may be observed (330 kDa).
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
      Please view the datasheet pdf for suggested dilutions.

    Additional Thyroglobulin Formats

    FormatsApplicationsSizes available
    Thyroglobulin: PurifiedE1 mg
    • Copyright © 2014 Bio-Rad AbD Serotec

    Recommended Secondary Antibody

    Recommended Negative Isotype Control

    Useful Reagents

    Recommended Positive Controls

    Histology Controls

      • Product Images

      • Sandwich ELISA analysis of human thyroglobulin using Mouse anti Human thyroglobulin , clone 5F9 (<a rel="nofollow" href='http://www.abdserotec.com/human-thyroglobulin-antibody-5f9-bgn-6e10-8900-0700.html'target="_blank">8900-0700</a>) as a capture reagent and biotinylated Mouse anti Human thyroglobulin, clone 5E6 (<a rel="nofollow" href='http://www.abdserotec.com/human-thyroglobulin-antibody-5e6-bgn-6d7-8900-0730.html'target="_blank">8900-0730</a>) as a detection reagent with purified human thyroglobulin as antigen (PHP236) for production of a standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (<a rel="nofollow" href='http://www.bio-rad.com/en-us/sku/168-1135-imark-microplate-absorbance-reader'target="_blank">Bio-Rad Catalog 168-1135</a>). Serum samples (green and blue) and plasma samples (orange and red) are shown

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