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CD102 antibody | mIC2/4 (3C4)

Rat anti Mouse CD102:Low Endotoxin

Product Type
Monoclonal Antibody
Clone
mIC2/4 (3C4)
Isotype
IgG2a
Specificity
CD102

Product Code Applications Pack Size List Price Your Price Qty
MCA2295EL
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F FN IP 0.5 mg loader
List Price Your Price
loader

Rat anti Mouse CD102 antibody, clone mIC2/4 (3C4) recognizes murine intercellular adhesion molecule-2 (ICAM-2), also known as CD102. Murine CD102 is a glycosylated cell surface protein that is broadly expressed on most leucocytes and is constitutively expressed on endothelial cells. CD102 interacts with the CD11a/CD18 integrin LFA-1. Reports suggest that CD102 may play a role in lymphocyte re-circulation and T-cell activation.

Rat anti Mouse CD102 antibody, clone mIC2/4 is reported to inhibit interactions between CD102 and LFA-1 (Xuet al. 1996).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
COS cells transfected with murine ICAM-2.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Endotoxin Level
< 0.01 EU/μg
Fusion Partners
Spleen cells from immunised Lewis rats were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Functional Assays
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106cells in 100μl

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Description Rat IgG2a Negative Control:Low Endotoxin

Source Reference

  1. Xu, H. et al. (1996) Characterization of murine intercellular adhesion molecule-2.
    J Immunol. 156 (12): 4909-14.

References for CD102 antibody

  1. Jones, D.T. et al. (2013) Endogenous ribosomal protein L29 (RPL29): a newly identified regulator of angiogenesis in mice.
    Dis Model Mech. 6 (1): 115-24.

Synonyms
ICAM-2
RRID
AB_566422
UniProt
P35330
Entrez Gene
Icam2
GO Terms
GO:0001931 uropod
GO:0005887 integral to plasma membrane
GO:0016337 cell-cell adhesion

MCA2295EL

1611

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