Browsing Modes for Business Users

CD134 antibody | OX-86

Product Code Applications Pack Size List Price Your Price Qty
MCA1420
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.25 mg loader
List Price Your Price
loader

Rat anti Mouse CD134 antibody, clone OX-86 recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.

Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
CHO cells transfected with murine OX40.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized AO rats were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
List Price Your Price
loader
Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed) STAR71D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CD134 antibody

  1. Higgins, L.M. et al. (1999) Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.
    J Immunol. 162 (1): 486-93.
  2. Malmström, V. et al. (2001) CD134L expression on dendritic cells in the mesenteric lymph nodes drives colitis in T cell-restored SCID mice.
    J Immunol. 166: 6972-81.
  3. McHugh, R.S. et al. (2002) CD4(+)CD25(+) immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor.
    Immunity. 16: 311-23.
  4. Lavelle, E.C. et al. (2003) Cholera toxin promotes the induction of regulatory T cells specific for bystander antigens by modulating dendritic cell activation.
    J Immunol. 171 (5): 2384-92.
  5. Andarini, S. et al. (2004) Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.
    Cancer Res. 64: 3281-7.
  6. Lee, S.J. et al. (2004) 4-1BB and OX40 dual costimulation synergistically stimulate primary specific CD8 T cells for robust effector function.
    J Immunol. 173 (5): 3002-12.
  7. Zaini, J. et al. (2007) OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.
    J Clin Invest. 117: 3330-8.
  8. Thaunat, O. et al. (2010) Immune responses elicited in tertiary lymphoid tissues display distinctive features.
    PLoS One. 5: e11398.
  9. View The Latest Product References
  10. Maenz, M. et al. (2011) A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
    Mol Vis. 17: 420-9.
  11. Wythe, S.E. et al. (2012) OX40 Ligand and Programmed Cell Death 1 Ligand 2 Expression on Inflammatory Dendritic Cells Regulates CD4 T Cell Cytokine Production in the Lung during Viral Disease
    J Immunol. 188: 1647-55.
  12. Reynolds, C. et al. (2014) Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
    BMC Biol. 12: 32.

Further Reading

  1. Takeda, K. et al. (2000) CD27-mediated activation of murine NK cells.
    J Immunol. 164 (4): 1741-5.

Flow Cytometry

Immunohistology - Frozen

Synonyms
OX40
RRID
AB_321575
UniProt
P47741
Entrez Gene
Tnfrsf4
GO Terms
GO:0016021 integral to membrane
GO:0005031 tumor necrosis factor receptor activity
GO:0006954 inflammatory response
GO:0006968 cellular defense response
GO:0009897 external side of plasma membrane
GO:0042981 regulation of apoptosis
GO:0030890 positive regulation of B cell proliferation
GO:0032582 negative regulation of gene-specific transcription
GO:0043433 negative regulation of transcription factor activity
GO:0045859 regulation of protein kinase activity
GO:0050710 negative regulation of cytokine secretion
GO:0051024 positive regulation of immunoglobulin secretion

MCA1420

147818

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD134 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products
Please note