CD51 Antibody | RMV-7

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  • Rat anti Mouse CD51:Alexa Fluor® 488
  • Rat anti Mouse CD51:RPE
  • Rat anti Mouse CD51
  • Rat anti Mouse CD51:Alexa Fluor® 647
  • Rat anti Mouse CD51:Alexa Fluor® 488
  • Rat anti Mouse CD51:Low Endotoxin
  • Rat anti Mouse CD51:Alexa Fluor® 647
  • Rat anti Mouse CD51
  • Rat anti Mouse CD51
(Rated 0.0/5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    RMV-7
  • Isotype
    IgG1
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2461GAC, F, IP0.1 mg
    MCA2461GA
    MCA2461C, F, IP0.25 mg
    MCA2461
    MCA2461ELF, FN, IP0.5 mg
    MCA2461EL
    MCA2461PEF100 Tests
    MCA2461PE
    MCA2461A488F100 Tests/1ml
    MCA2461A488
    MCA2461A647F100 Tests/1ml
    MCA2461A647
    MCA2461TC, F, IP25 µg
    MCA2461T
    MCA2461A647TF25 Tests/0.25ml
    MCA2461A647T
    MCA2461A488TF25 Tests/0.25ml
    MCA2461A488T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD51 antibody, clone RMV-7 recognizes murine CD51, a 140 kD alpha subunit of the vitronectin receptor, which is otherwise known as the integrin alpha v chain. CD51 can form heterodimers at the cell surface with a variety of beta integrins including CD29 and CD61.

      Heterodimers of CD51/CD61 function as a receptor for vitronectin, and a wide array of RGD-containing proteins including fibronectin, fibrinogen, von Willebrand factor, laminin, thrombospondin and osteopontin. CD51/CD61 is primarily expressed on myeloid cells and activated T-cells. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing.

      The RMV-7 antibody has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity (Takahashi et al. 1990).
    • Target Species
      Mouse
    • Product Form
      Purified IgG - conjugated to Alexa Fluor®488 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG - conjugated to Alexa Fluor®647 - liquid
      Purified IgG - conjugated to Alexa Fluor®488 - liquid
      Purified IgG - liquid
      Purified IgG - conjugated to Alexa Fluor®647 - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Preservative Stabilisers
      0.09% Sodium Azide
      1% Bovine Serum Albumin
      0.09% Sodium Azide
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide
      1% Bovine Serum Albumin
      0.09% Sodium Azide
      1% Bovine Serum Albumin
      None present
      0.09% Sodium Azide
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Cultured LAK cells from Balb/c mice.
    • Approx. Protein Concentrations
      IgG concentration 0.05mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised SD rats were fused with the cells of the P3X.63Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      receptor activity
      apoptotic cell clearance
      external side of plasma membrane
      angiogenesis
      integrin complex
      cell differentiation
      cell adhesion
      integrin-mediated signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
      The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
      The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
      The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
      The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/25Pack Size: 0.25 mg, 25 µg
      1/10Pack Size: 0.1 mg
      1/100
      Immunohistology - Frozen1/251/100
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
      Functional Assays
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/25Pack Size: 0.25 mg, 25 µg
      1/10Pack Size: 0.1 mg
      1/100
      Immunohistology - Frozen1/251/100
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/25Pack Size: 0.25 mg, 25 µg
      1/10Pack Size: 0.1 mg
      1/100
      Immunohistology - Frozen1/251/100
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      Please view the datasheet pdf for suggested dilutions.
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.

    Additional CD51 Antibody Formats

    FormatsCloneApplicationsSizes available
    CD51 Antibody : Alexa Fluor® 488RMV-7F100 Tests/1ml | 25 Tests/0.25ml
    CD51 Antibody : Alexa Fluor® 647RMV-7F25 Tests/0.25ml | 100 Tests/1ml
    CD51 Antibody : Low EndotoxinRMV-7F, FN, IP0.5 mg
    CD51 Antibody : PurifiedRMV-7C, F, IP25 µg | 0.25 mg | 0.1 mg
    CD51 Antibody : RPERMV-7F100 Tests
    • Copyright © 2014 Bio-Rad AbD Serotec

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        Useful Reagents

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        Histology Controls

        References

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