Mouse Seroblock FcR | FCR4G8

Hamster anti Mouse TCR Gamma/DeltaRPE antibody (MCA1366PE) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (BUF041B)

Hamster anti Mouse TCR Gamma/DeltaRPE antibody (MCA1366PE) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (BUF041B)

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  • Hamster anti Mouse TCR Gamma/DeltaRPE antibody (MCA1366PE) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (BUF041B)
  • Mouse Seroblock FcR
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  • Product Type
    Accessory Reagent
  • Clone
    FCR4G8
  • Isotype
    IgG2b
1 Format Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF041AF0.1 mg
    BUF041A
    BUF041BF0.5 mg
    BUF041B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • BUF041A is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRgammaIII and FcRgammaII, respectively.

      The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes.

      The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in “high background” staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with BUF041A, resulting in clearer staining.

      For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MCA2305F), AlexaFluor488 (MCA2305A488), AlexaFluor647 (MCA2305A647), Biotin (MCA2305B) and RPE (MCA2305PE).
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
    • Immunogen
      PU5 1.8 IOE7 Balb/c mouse cell line
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNo
      Flow Cytometry
    • Application NameYesNo
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      Please view the datasheet pdf for suggested dilutions.
    • Instructions For Use
      In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

      1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of BUF041B (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.

      2) Add test antibody according to manufacturers instructions – Do not wash BUF041B off the cells. BUF041B is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*

      3) Proceed with staining as usual.

      * Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. AbD Serotec supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose.
    • Instructions For Use
      In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

      1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of BUF041A (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.

      2) Add test antibody according to manufacturers instructions – Do not wash BUF041A off the cells. BUF041A is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*

      3) Proceed with staining as usual.

      * Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. AbD Serotec supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose.

    Additional Mouse Seroblock FcR Formats

    FormatsCloneApplicationsSizes available
    Mouse Seroblock FcR : ReagentFCR4G8F0.5 mg | 0.1 mg
    • Copyright © 2014 Bio-Rad AbD Serotec

    Recommended Secondary Antibody

    Recommended Negative Isotype Control

    Useful Reagents

    Recommended Positive Controls

    Histology Controls

      • Product Images

      • Hamster anti Mouse TCR Gamma/DeltaRPE antibody (MCA1366PE) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (BUF041B)

    References

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