Antibody Conjugation

High-quality, reproducible antibody conjugation is essential for consistent experimental results. Our proprietary purification processes have been developed to maximize the yield, purity, and immunoreactivity of your antibodies.

  • Conjugation of small quantities through to industrial scale large batches.
  • Extensive range of markers for conjugation: including alkaline phosphatase, biotin, HRP and the fluorescent dyes summarised below.

 

Dye

Laser
excitation
line (nm)

Maximal absorbance (nm)

Maximal emission (nm)

Suggested laser

Fluores- cence
color
Pacific Blue™*†(1) 405, 407 416 456 Violet diode  
FITC 488 495 525 Argon-Ion  
RPE 488 490, 565 578 Argon-Ion  
APC 633, 635, 647 650 661 Krypton-Argon  
DyLight® 405 405 400 420 Violet diode  
DyLight® 488 488 493 518 Argon-Ion  
DyLight® 549(2) 550 562 576 Krypton-Argon  
DyLight® 649(2) 646 654 673 Krypton-Argon  
DyLight® 680(2) 682 680 715 Argon-Ion  
DyLight® 750(2) 752 750 778 Argon-Ion  
DyLight® 800 770 777 794 DPSS  

(2) Not for sale in the USA and Canada
(1) For conjugation to AbD Serotec primary antibodies only.
* Can only be used on flow cytometers with a violet laser (402–415 nm range).
† Low spectral overlap with green fluorochromes e.g. FITC.
# Caution: if you intend to use PE-Alexa Fluor® 647 in conjunction with APC or Alexa Fluor® 647, please be aware of the possibility of excitation in the tandem dye directly by the 633/635/647 laser.
DPSS is a diode-pumped solid state laser

Alexa Fluor® and Pacific Blue™ are trademarks of Molecular Probes Inc, OR, USA.
 DyLight® is a trademark of Thermo Fisher Scientific and its subsidiaries.