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CD117 antibody | 2B8/BM

Product Code Applications Pack Size List Price Your Price Qty
MCA2598GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP * WB * 0.1 mg loader
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loader

Mouse anti Pig CD117, clone 2B8/BM is specific for porcine CD117, also known as c-kit, a 155 kDa type I transmembrane protein with protein tyrosine kinase activity, which plays an important role in early hematopoiesis (Perez et al. 2007).

Hematopoietic stem cells (HSC) of bone marrow have multilineage differentiation potential and an extensive capacity for self-renewal. The majority of adult bone marrow hematopoietic progenitor cells are CD117+ and have been used successfully in xenograft transplantation models for long term survival of grafts, without symptoms of graft-versus-host disease (GVHD).

Mouse anti pig CD117, clone 2B8/BM recognizes CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells.

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine bone marrow cells (BMC).
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Immunoprecipitation 1
Western Blotting 2
  1. 1Enrichment of 2B8/BM+ BMC population required by depletion of CD3+, 6D10+ and CD163+ cells. Please see Pérez, C. et al. (2007) for details.
  2. 2Enrichment of 2B8/BM+ BMC population required by depletion of CD3+, 6D10+ and CD163+ cells. Please see Pérez, C. et al. (2007) for details.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blotting
Clone 2B8/BM detects a band of approximately 155kDa in enriched porcine bone marrow cell lysates under non-reducing conditions.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

Source Reference

  1. Pérez C et al. (2007) Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM.
    J Immunol Methods. 321 (1-2): 70-9.

References for CD117 antibody

  1. Hatzistergos, K.E. et al. (2010) Bone marrow mesenchymal stem cells stimulate cardiac stem cell proliferation and differentiation.
    Circ Res. 107: 913-22.
  2. Escalona, Z. et al. (2014) Molecular characterization and expression of porcine Siglec-5.
    Dev Comp Immunol. 44 (1): 206-16.
  3. Wehman, B. et al. (2016) Mesenchymal stem cells preserve neonatal right ventricular function in a porcine model of pressure overload.
    Am J Physiol Heart Circ Physiol. 310 (11): H1816-26.
  4. Wehman, B. et al. (2016) Intracoronary Stem Cell Delivery to the Right Ventricle: A Preclinical Study.
    Semin Thorac Cardiovasc Surg. 28 (4): 817-24.
  5. Wehman, B. et al. (2017) Cardiac Progenitor Cells Enhance Neonatal Right Ventricular Function After Pulmonary Artery Banding.
    Ann Thorac Surg. 104 (6): 2045-53.
  6. Arenal, Á. et al. (2022) Effects of Cardiac Stem Cell on Postinfarction Arrhythmogenic Substrate.
    Int J Mol Sci. 23 (24): 16211.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Synonyms
C-KIT
UniProt
Q2HWD6
Entrez Gene
KIT
GO Terms
GO:0016021 integral to membrane
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005524 ATP binding

MCA2598GA

162887 1803

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