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Rituximab antibody | AbD18423_hIgG1

Human anti Rituximab

Product Type
Monoclonal Antibody
Clone
AbD18423_hIgG1
Isotype
IgG1
Specificity
Rituximab

Product Code Applications Pack Size List Price Your Price Qty
HCA201
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. Clone AbD18423_hIgG1 is an anti-idiotypic antibody that specifically recognises the rituximab humanised monoclonal antibody. This fully human antibody can be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera

Product Form
Human IgG1 antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Source
HKB-11
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Rituximab
Affinity
The intrinsic affinity of the monovalent form of this antibody is KD=0.13 nM as measured by real time, label-free molecular interaction analysis on immobilized rituximab.
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product may be used in a direct ELISA or as a detection reagent, when conjugated to HRP in a sandwich ELISA .
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay. Protocol: ADA bridging ELISA.

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Synonyms
MabThera®
Rituxan®
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

HCA201

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