This item has been added to your basket.- 5-METHYLCYTIDINE Antibody
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Product CodeMCA2201
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DescriptionMOUSE ANTI 5-METHYLCYTIDINE
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Product TypeMonoclonal Antibody
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Clone33D3
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IsotypeIgG1
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FormatPurified
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ApplicationsC, F, IB, IF, MP, P *
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Pack Size0.1 mg
Product Summary
Additional Formats
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Application Notes
References
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5-METHYLCYTIDINE Antibody Product Summary
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Specificity
MCA2201 recognises the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression.
Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine.
MCA2201 has been reported for use in Methylated DNA immunoprecipitation (MeDIP).
Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine.
MCA2201 has been reported for use in Methylated DNA immunoprecipitation (MeDIP).
Target Species
Broad
Species Cross-Reactivity
| Target Species | Cross Reactivity |
|---|---|
| Human | Yes |
| Mouse | Yes |
| Rat | Yes |
Product Form
Purified
Preservative Stabilisers
| 0.01% | Thiomersal |
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
5-METHYLCYTIDINE Antibody Additional Formats
5-METHYLCYTIDINE Antibody Related Reagents
5-METHYLCYTIDINE Antibody More Images
5-METHYLCYTIDINE Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry | ||||
| Immunoblotting | ||||
| Immunofluorescence | ||||
| Immunohistology - Frozen | ||||
| Immunohistology - Paraffin(1) | ||||
| Immunoprecipitation | ||||
| Methylated DNA Immunoprecipitation | ||||
| Western Blotting |
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Membrane permeabilization may be required for this application. Cell pretreatment before staining is described in reference 4. Giraldo, A. M. et al.
Membrane permeabilization may be required for this application. Cell pretreatment before staining is described in reference 4. Giraldo, A. M. et al.
5-METHYLCYTIDINE Antibody References
References
1. Reynaud, C. et al. (1992) Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies. Cancer Lett. 61: 255-262.
2. Habib, M. et al. (1999) DNA Global Hypomethylation in EBV - Transformed Interphase Nuclei. Exp. Cell. Res. 249: 46-53.
3. Hernandez-Blazquez, F. et al. (2000) Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gut. 47: 689-693.
4. Giraldo, A. M. et al. (2007) DNA methylation and histone acetylation patterns in cultured bovine fibroblasts
for nuclear transfer.
Mol. Reprod. Dev. 74: 1514-1524.
5. Shen, R. et al. (2009) Reversibility of aberrant global DNA and estrogen receptor-alpha gene methylation distinguishes colorectal precancer from cancer.Int J Clin Exp Pathol. 2:21-33.
6. Pontes, O. et al. (2007) Postembryonic establishment of megabase-scale gene silencing in nucleolar dominance.PLoS One. 2: e1157.
7. Yang, F. et al. (2010) Trichostatin A and 5-azacytidine both cause an increase in global histone H4 acetylation and a decrease in global DNA and H3K9 methylation during mitosis in maize.BMC Plant Biol. 10: 178.
8. Suter, J.D. et al. (2010) Label-free DNA methylation analysis using opto-fluidic ring resonators.Biosens Bioelectron. 26: 1016-20.
9. Li, Y. and O'Neill, C. (2012) Persistence of cytosine methylation of DNA following fertilisation in the mouse.PLoS One. 7:e30687.
5-METHYLCYTIDINE Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag 14 myeloma cell line.
Health And Safety Information
Material Safety Datasheet Documentation #10094 available at:
http://www.abdserotec.com/uploads/MSDS/10094.pdf
http://www.abdserotec.com/uploads/MSDS/10094.pdf
Regulatory
For research purposes only
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