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HCA040P
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  • IgM Antibody
  • Product Code
    HCA040P
  • Description
    HUMAN ANTI MOUSE IgM:HRP
  • Product Type
    Monoclonal Antibody
  • Clone
    4620
  • Isotype
    HuCAL Fab bivalent
  • Format
    HRP
  • Applications
    E
  • Pack Size
    0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

IgM Antibody Product Summary

Specificity

HCA040 is specific for mouse IgM.

No cross reactivity is seen with other mouse immunoglobulin sub-classes, rat immunoglobulins, or sheep, bovine and goat IgG.

Target Species

Mouse

Product Form

A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain and is conjugated to horseradish peroxidase.

Preparation

Metal chelate affinity chromatography

Preservative Stabilisers

0.01%Thiomersal

Immunogen

Purified mouse monoclonal IgM

Approx. Protein Concentrations

Antibody concentration 1mg/ml

Buffer Solution

Phosphate buffered saline

IgM Antibody Additional Formats

  • FormatProduct CodePack SizeApplicationsList PriceQuantity
    FITCHCA040F0.1 mgF
    HCA040F
    PurifiedHCA0400.1 mgE, F
    HCA040
    PurifiedHCA040A50 µgE, F
    HCA040A

IgM Antibody Related Reagents

Useful Reagents

FormatProduct CodePack SizeApplicationsList PriceQuantity
Ready To UseBUF042A100 mlE
BUF042A
Ready To UseBUF052A100 mlC, E, P, WB
BUF052A
Ready To UseBUF054A100 mlE
BUF054A
Ready To UseBUF055A100 mlE
BUF055A
Ready To UseBUF056A100 mlE
BUF056A
Ready To UseBUF057A100 mlE
BUF057A
Ready To UseBUF062A100 mlE
BUF062A
Ready To UseBUF052C1000 mlC, E, P, WB
BUF052C
Ready To UseBUF052B500 mlC, E, P, WB
BUF052B

More Reagents With This Specificity

IgM Antibody More Images

IgM Antibody Application Notes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
Application NameYesNoNot DeterminedSuggested Dilution
ELISA1/100 - 1/500
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

IgM Antibody References

IgM Antibody More Information

Storage

Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.

Shelf Life

12 months from date of despatch.

Licensed Use

For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.

GO Terms

UniProt

Health And Safety Information

Material Safety Datasheet Documentation #10094 available at:
http://www.abdserotec.com/uploads/MSDS/10094.pdf

Acknowledgements

Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

Regulatory

For research purposes only