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C-MYC Antibody
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Product CodeMCA2200A647
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DescriptionMouse Anti c-Myc:Alexa Fluor® 647
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Product TypeMonoclonal Antibody
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Clone9E10
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IsotypeIgG1
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FormatALEXA FLUOR® 647
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ApplicationsF *
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Pack Size100 TESTS/1ml
Product Summary
Additional Formats
Related Reagents
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Application Notes
References
More Information
C-MYC Antibody Product Summary
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Specificity
MCA2200A647 detects the 62kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.
This antibody may also be used to detect the commonly used c-myc 'tag'.
This antibody may also be used to detect the commonly used c-myc 'tag'.
Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
| 0.09% | Sodium Azide |
| 1% | Bovine Serum Albumin |
Immunogen
Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Buffer Solution
Phosphate buffered saline
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the myeloma cell line.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
C-MYC Antibody Additional Formats
Format Product Code Pack Size Applications List Price Quantity ALEXA FLUOR® 488 MCA2200A488 100 TESTS/1ml F * MCA2200A488Alk. Phos. MCA2200A 0.1 mg C, E, P, WB* MCA2200ABiotin MCA2200B 0.1 mg C, E, F *, P, WB* MCA2200BFITC MCA2200F 0.1 mg F * MCA2200FHRP MCA2200P 0.1 mg C, E, P, WB* MCA2200PPurified MCA2200GA 0.1 mg C, E, F *, P MCA2200GAPurified MCA2200 1 mg C, E, F *, P, WB* MCA2200Purified MCA2200G 2 mg C, E, F *, P, WB* MCA2200GPurified MCA2200T 20 µg C, F *, P MCA2200TRPE MCA2200PE 100 TESTS F * MCA2200PE
C-MYC Antibody Related Reagents
Recommended Negative Isotype Control
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| ALEXA FLUOR® 647 | MCA928A647 | 100 TESTS/1ml | F | MCA928A647 |
More Reagents With This Specificity
C-MYC Antibody More Images
C-MYC Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| Flow Cytometry(1) | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
(1)
Membrane permeabilisation is required for this application. Serotec recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
C-MYC Antibody References
References
1. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc. proto-oncogene product.Mol. Cell. Biol. 5: 3610 -3616.
2. Spandidos, D. A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res. 7: 1299 -1304.
3. Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae.Microb. Cell Fact. 9: 74-86.
4. Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction.Biochem J. 404: 23-9.
5. Head, B. et al. (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells.J Cell Biol. 187: 959-66.
6. Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.Protein Eng. 14: 803-6.
7. Krauss, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.Proteins. 73: 552-65.
8. Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling.J Immunol. 184: 6359-66.
9. Duriseti, S. et al. (2010) Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration.J Biol Chem. 285: 26878-88.
10. Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.Blood. 106: 2936-43.
11. Wallace, S.W. et al. (2010) Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B.Mol Biol Cell. 21: 2996-3006.
C-MYC Antibody More Information
GO Terms
UniProt
Health And Safety Information
Material Safety Datasheet Documentation #10041 available at:
http://www.abdserotec.com/uploads/MSDS/10041.pdf
http://www.abdserotec.com/uploads/MSDS/10041.pdf
Acknowledgements
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Regulatory
For research purposes only
© Bio-Rad Laboratories, Inc
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