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- C-MYC Antibody
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Product CodeMCA2200G
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DescriptionMOUSE ANTI C-MYC
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Product TypeMonoclonal Antibody
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Clone9E10
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IsotypeIgG1
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FormatPurified
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ApplicationsC, E, F *, P, WB*
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Pack Size2 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
C-MYC Antibody Product Summary
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Specificity
MCA2200G detects the 62kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.
This antibody may also be used to detect the commonly used c-myc 'tag'.
This antibody may also be used to detect the commonly used c-myc 'tag'.
Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilisers
| 0.09% | Sodium Azide |
Immunogen
Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
C-MYC Antibody Additional Formats
Format Product Code Pack Size Applications List Price Quantity ALEXA FLUOR® 488 MCA2200A488 100 TESTS/1ml F * MCA2200A488ALEXA FLUOR® 647 MCA2200A647 100 TESTS/1ml F * MCA2200A647Alk. Phos. MCA2200A 0.1 mg C, E, P, WB* MCA2200ABiotin MCA2200B 0.1 mg C, E, F *, P, WB* MCA2200BFITC MCA2200F 0.1 mg F * MCA2200FHRP MCA2200P 0.1 mg C, E, P, WB* MCA2200PPurified MCA2200GA 0.1 mg C, E, F *, P MCA2200GAPurified MCA2200 1 mg C, E, F *, P, WB* MCA2200Purified MCA2200T 20 µg C, F *, P MCA2200TRPE MCA2200PE 100 TESTS F * MCA2200PE
C-MYC Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Alk. Phos. | STAR117A | 0.5 mg | E, WB | STAR117A | |
| Alk. Phos. | STAR87A | 1 mg | C, E, WB | STAR87A | |
| Biotin | AAC10B | 0.5 mg | C, E, P, WB | AAC10B | |
| DyLight®800 | STAR117D800GA | 0.1 mg | F, IF, WB | STAR117D800GA | |
| DyLight®800 | STAR8D800GA | 0.1 mg | F, IF, WB | STAR8D800GA | |
| FITC | STAR117F | 0.5 mg | F | STAR117F | |
| FITC | STAR70 | 0.5 mg | F | STAR70 | |
| FITC | AAC10F | 1 mg | C | AAC10F | |
| FITC | STAR120F | 1 mg | C, F | STAR120F | |
| FITC | STAR9B | 1 mg | F | STAR9B | |
| HRP | HCA036P | 0.1 mg | E | HCA036P | |
| HRP | STAR117P | 0.5 mg | E, WB | STAR117P | |
| HRP | STAR77 | 0.5 mg | C, E, P | STAR77 | |
| HRP | STAR120P | 1 mg | E, WB | STAR120P | |
| HRP | STAR13B | 1 mg | C, E, P, RE, WB | STAR13B | |
| HRP | STAR87P | 1 mg | E | STAR87P | |
| RPE | STAR12A | 100 TESTS | F | STAR12A | |
| RPE | STAR76 | 100 TESTS | F | STAR76 |
Recommended Negative Isotype Control
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Purified | MCA928 | 100 TESTS | C, F | MCA928 |
More Reagents With This Specificity
C-MYC Antibody More Images
C-MYC Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | 1/100 - 1/500 | |||
| Flow Cytometry(1) | 1/10 - 1/50 | |||
| Immunohistology - Frozen | ||||
| Immunohistology - Paraffin | ||||
| Immunoprecipitation | ||||
| Western Blotting(2) | 1/100 - 1/500 |
(1)
Membrane permeabilisation is required for this application. Serotec recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
(2)
9E10 recognizes c-myc under non-reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
C-MYC Antibody References
References
1. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc. proto-oncogene product.Mol. Cell. Biol. 5: 3610 -3616.
2. Spandidos, D. A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res. 7: 1299 -1304.
3. Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae.Microb. Cell Fact. 9: 74-86.
4. Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction.Biochem J. 404: 23-9.
5. Head, B. et al. (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells.J Cell Biol. 187: 959-66.
6. Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.Protein Eng. 14: 803-6.
7. Krauss, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.Proteins. 73: 552-65.
8. Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling.J Immunol. 184: 6359-66.
9. Duriseti, S. et al. (2010) Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration.J Biol Chem. 285: 26878-88.
10. Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.Blood. 106: 2936-43.
11. Wallace, S.W. et al. (2010) Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B.Mol Biol Cell. 21: 2996-3006.
C-MYC Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.
GO Terms
UniProt
Health And Safety Information
Material Safety Datasheet Documentation #10040 available at:
http://www.abdserotec.com/uploads/MSDS/10040.pdf
http://www.abdserotec.com/uploads/MSDS/10040.pdf
Regulatory
For research purposes only
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