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IgG Antibody
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Product Code2AB02B
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DescriptionSheep Anti Rabbit Igg:Biotin
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Product TypePolyclonal Antibody
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IsotypePolyclonal IgG
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FormatBiotin
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ApplicationsC, E, P, WB
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Pack Size1 ml
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
IgG Antibody Product Summary
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Specificity
2AB02B recognises rabbit IgG (Heavy and light chains).
Cross reaction with bovine, human, mouse or rat serum has been minimised by adsorption.
Cross reaction with bovine, human, mouse or rat serum has been minimised by adsorption.
Target Species
Rabbit
Species Cross-Reactivity
| Target Species | Cross Reactivity |
|---|---|
| Bovine | No |
| Human | No |
| Mouse | No |
| Rat | No |
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Preservative Stabilisers
| 0.09% | Sodium Azide |
| 1% | Bovine Serum Albumin |
Immunogen
Rabbit IgG from pooled sera
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
IgG Antibody Additional Formats
Format Product Code Pack Size Applications List Price Quantity Purified 2AB02 1 ml ID 2AB02
IgG Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| APC | STAR119 | 100 TESTS | F | STAR119 |
More Reagents With This Specificity
IgG Antibody More Images
IgG Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | 1/25600 - 1/51200 | |||
| Flow Cytometry | ||||
| Immunohistology - Frozen | 1/400 - 1/800 | |||
| Immunohistology - Paraffin | 1/100 - 1/200 | |||
| Western Blotting | 1/20000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
IgG Antibody References
References
1. Blakey, D. et al. (2002) Antitumor activity of the novel vascular targeting agent ZD6126 in a panel of tumor modelsClin. Cancer Res. 8 : 1974 - 1983.
2. Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infectioInfect Immun. 78: 3578-84.
3. Pimanda, J.E. et al. (2006) Transcriptional link between blood and bone: the stem cell leukemia gene and its +19 stem cell enhancer are active in bone cells.Mol Cell Biol. 26: 2615-25.
4. Dell'Accio, F. et al. (2006) Activation of WNT and BMP signaling in adult human articular cartilage following mechanical injury.Arthritis Res Ther. 8: R139.
5. Clements, K.M. et al. (2011) Matrix metalloproteinase 17 is necessary for cartilage aggrecan degradation in an inflammatory environment.Ann Rheum Dis. 70: 683-9.
IgG Antibody More Information
GO Terms
UniProt
Health And Safety Information
Material Safety Datasheet Documentation #10041 available at:
http://www.abdserotec.com/uploads/MSDS/10041.pdf
http://www.abdserotec.com/uploads/MSDS/10041.pdf
Regulatory
For research purposes only
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