This item has been added to your basket.- IgG Antibody
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Product CodeAAC10B
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DescriptionSHEEP ANTI MOUSE IgG (H/L):Biotin
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Product TypePolyclonal Antibody
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IsotypePolyclonal IgG
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FormatBiotin
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ApplicationsC, E, P, WB
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Pack Size0.5 mg
Product Summary
Additional Formats
Related Reagents
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Application Notes
References
More Information
IgG Antibody Product Summary
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Specificity
AAC10B recognises mouse IgG heavy and light chains.
Does not cross react with murine IgM.
Does not cross react with murine IgM.
Target Species
Mouse
Species Cross-Reactivity
| Target Species | Cross Reactivity |
|---|---|
| Bovine | No |
| Human | No |
| Rabbit | No |
Product Form
Purified IgG conjugated to Biotin - liquid
Preservative Stabilisers
| 0.09% | Sodium Azide |
| 1% | Bovine Serum Albumin |
Immunogen
Polyclonal mouse IgG
Approx. Protein Concentrations
1 mg/ml
Buffer Solution
Phosphate buffered saline
IgG Antibody Additional Formats
IgG Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| APC | STAR119 | 100 TESTS | F | STAR119 |
More Reagents With This Specificity
IgG Antibody More Images
IgG Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | 1/102400 - 1/204800 | |||
| Immunohistology - Frozen | 1/40 - 1/80 | |||
| Immunohistology - Paraffin | 1/40 - 1/80 | |||
| Western Blotting | 1/20000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
IgG Antibody References
References
1. Hsiao, Y. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells. Vet. Immunol. Immunopathol.87: 19-27.
2. Baur, K. etal (2010) Immediate-Early Expression of a Recombinant Antigen by Modified Vaccinia Virus Ankara Breaks the Immunodominance of Strong Vector-Specific B8R Antigen in Acute and Memory CD8 T-Cell Responses. J Virol. 84: 8743-52.
3. Pacheco, J.M. et al. (2010) IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination.Clin Vaccine Immunol. 17: 550-8.
IgG Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
GO Terms
UniProt
Health And Safety Information
Material Safety Datasheet Documentation #10041 available at:
http://www.abdserotec.com/uploads/MSDS/10041.pdf
http://www.abdserotec.com/uploads/MSDS/10041.pdf
Regulatory
For research purposes only
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AbD Serotec | Antibody Supplier and Manufacturer
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