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IgG Antibody
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Product CodeSTAR9B
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DescriptionRabbit F(ab')2 Anti Mouse Igg:FITC
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Product TypePolyclonal Antibody
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IsotypePolyclonal IgG
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FormatFITC
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ApplicationsF
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Pack Size1 mg
Product Summary
Additional Formats
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Application Notes
References
More Information
IgG Antibody Product Summary
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Specificity
Mouse IgG (all subclasses).
Cross reactivity with mouse IgM and IgA is expected.Cross reactivity with rat IgG is expected. Cross reactivity with human serum proteins has been minimised by solid phase adsorption.
Cross reactivity with mouse IgM and IgA is expected.Cross reactivity with rat IgG is expected. Cross reactivity with human serum proteins has been minimised by solid phase adsorption.
Target Species
Mouse
Species Cross-Reactivity
| Target Species | Cross Reactivity |
|---|---|
| Rat | Yes |
Product Form
F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer I (FITC) - liquid
Preservative Stabilisers
| 0.09% | Sodium Azide |
Immunogen
Purified mouse IgG
Approx. Protein Concentrations
F(ab')2 concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
IgG Antibody Additional Formats
IgG Antibody Related Reagents
IgG Antibody More Images
IgG Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| Flow Cytometry | 1/25 - 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.
IgG Antibody References
References
1. O-Charoenrat, P. et al. (2000) Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells. Cancer Res. 60: 1121-1128.
2. Lamote, I. et al. (2004) Influence of 17beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes.J. Dairy Sci. 87: 3340-3349
3. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
Blood. 112: 2512-2519.
4. Fleming, E.H. et al. (2006) Respiratory syncytial virus F envelope protein associates with lipid rafts without a requirement for other virus proteins.J Virol. 80: 12160-70.
5. Peretti, M. et al. (2001) Expression of the three human major histocompatibility complex class II isotypes exhibits a differential dependence on the transcription factor RFXAP.Mol Cell Biol. 21: 5699-709.
6. Krawczyk, M. et al. (2005) New functions of the major histocompatibility complex class II-specific transcription factor RFXANK revealed by a high-resolution mutagenesis study.Mol Cell Biol. 25: 8607-18.
7. Frenzel, R. et al. (2006) The human thyrotropin receptor is predominantly internalized by beta-arrestin 2.Endocrinology. 147: 3114-22.
8. Brancaleone, V. et al. (2011) Evidence for an anti-inflammatory loop centered on polymorphonuclear leukocyte formyl peptide receptor 2/lipoxin A4 receptor and operative in the inflamed microvasculature.J Immunol. 186: 4905-14.
9. Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.J Leukoc Biol. 85: 44-54.
10. Maderna, P. et al. (2010) FPR2/ALX receptor expression and internalization are critical for lipoxin A4 and annexin-derived peptide-stimulated phagocytosis.FASEB J. 24: 4240-9.
11. Ioannou, N. et al. (2011) Anti-tumour activity of afatinib, an irreversible ErbB family blocker, in human pancreatic tumour cells.Br J Cancer. 105: 1554-62.
12. Renshaw, D. et al. (2010) Downstream gene activation of the receptor ALX by the agonist annexin A1.PLoS One. 5. pii: e12771.
13. Bena, S. et al. (2012) Annexin A1 interaction with the FPR2/ALX receptor: identification of distinct domains and downstream associated signaling.J Biol Chem. 287: 24690-7.
IgG Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Mouse IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
GO Terms
UniProt
Health And Safety Information
Material Safety Datasheet Documentation #10040 available at:
http://www.abdserotec.com/uploads/MSDS/10040.pdf
http://www.abdserotec.com/uploads/MSDS/10040.pdf
Regulatory
For research purposes only
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