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- MHC CLASS II DQ Antibody
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Product CodeMCA5655F
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DescriptionMOUSE ANTI BOVINE MHC CLASS II DQ:FITC
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Product TypeMonoclonal Antibody
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CloneCC158
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IsotypeIgG2a
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FormatFITC
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ApplicationsF
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Pack Size0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
MHC CLASS II DQ Antibody Product Summary
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Specificity
MCA5655F specifically recognises Bovine MHC Class II DQ. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.
The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.
The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.
Target Species
Bovine
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Buffer Solution
Phosphate buffered saline
MHC CLASS II DQ Antibody Additional Formats
MHC CLASS II DQ Antibody Related Reagents
Recommended Negative Isotype Control
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| FITC | MCA929F | 100 TESTS | F | MCA929F |
More Reagents With This Specificity
MHC CLASS II DQ Antibody More Images
MHC CLASS II DQ Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| Flow Cytometry |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. .
MHC CLASS II DQ Antibody References
References
1. Stephens S.A. (2002) Infection and transformation of dendritic cells from bovine
afferent lymph by Theileria annulataParasitology 124:485-493
2. Yamakawa Y. et al (2008) Identification and functional characterization of a Bovine orthologue to DC-SIGN.J. Leuk. Biol. 83:1369-1404
3. Price,S.J. and Hope, J.C. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.Immunology. 126: 201-8.
4. Van Rhijn, I. et al. (2007) Massive, sustained gammadelta T cell migration from the bovine skin in vivo.J Leukoc Biol. 81: 968-73.
MHC CLASS II DQ Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
UniProt
Health And Safety Information
Material Safety Datasheet Documentation #10041 available at:
http://www.abdserotec.com/uploads/MSDS/10041.pdf
http://www.abdserotec.com/uploads/MSDS/10041.pdf
Regulatory
For research purposes only
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