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- DECTIN-2 Antibody
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Product CodeMCA2415GA
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DescriptionRAT ANTI MOUSE DECTIN-2
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Product TypeMonoclonal Antibody
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CloneD2.11E4
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IsotypeIgG2a
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FormatPurified
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ApplicationsC, F, IP
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Pack Size0.1 mg
Product Summary
Additional Formats
Related Reagents
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Application Notes
References
More Information
DECTIN-2 Antibody Product Summary
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Specificity
MCA2415GA recognises mouse DC-associated C-type lectin-2 (Dectin-2), a type II transmembrane protein that is a member of the C-type lectin superfamily. In mouse, dectin-2 is predominantly expressed on tissue macrophages and some dendritic cells. Clone D2.11E4 does not stain brain microglia or meningeal macrophages.
Significant expression of Dectin-2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin-2 but transient up-regulation of expression has been demonstrated on monocytes at sites of inflammation.
The function of dectin-2 has not been fully determined but studies suggest a possible role in immune-surveillance.
Significant expression of Dectin-2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin-2 but transient up-regulation of expression has been demonstrated on monocytes at sites of inflammation.
The function of dectin-2 has not been fully determined but studies suggest a possible role in immune-surveillance.
Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
| 0.09% | Sodium Azide |
Immunogen
Dectin-2 (extracellular domain) Fc Fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
DECTIN-2 Antibody Additional Formats
Format Product Code Pack Size Applications List Price Quantity ALEXA FLUOR® 488 MCA2415A488 100 TESTS/1ml F MCA2415A488ALEXA FLUOR® 488 MCA2415A488T 25 TESTS/0.25ml F MCA2415A488TALEXA FLUOR® 647 MCA2415A647 100 TESTS/1ml F MCA2415A647ALEXA FLUOR® 647 MCA2415A647T 25 TESTS/0.25ml F MCA2415A647TBiotin MCA2415B 0.1 mg F MCA2415BBiotin MCA2415BT 25 µg F MCA2415BTFITC MCA2415F 0.1 mg F MCA2415FFITC MCA2415FT 25 µg F MCA2415FTLow Endotoxin MCA2415EL 0.5 mg C, F, IP MCA2415ELPurified MCA2415 0.25 mg C, F, IP MCA2415Purified MCA2415T 25 µg C, F, IP MCA2415TRPE MCA2415PE 100 TESTS F MCA2415PERPE MCA2415PET 25 TESTS F MCA2415PET
DECTIN-2 Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Alk. Phos. | STAR131A | 1 ml | C, E, P, WB | STAR131A | |
| Biotin | STAR131B | 0.5 mg | C, E, IF, P, WB | STAR131B | |
| DyLight®800 | STAR16D800GA | 0.1 mg | F, IF, WB | STAR16D800GA | |
| DyLight®800 | STAR71D800GA | 0.1 mg | F, IF, WB | STAR71D800GA | |
| FITC | STAR69 | 0.5 ml | F | STAR69 | |
| FITC | STAR80F | 0.7 mg | F | STAR80F | |
| FITC | STAR17B | 1 mg | F | STAR17B | |
| HRP | STAR72 | 0.5 mg | C, E, P | STAR72 | |
| HRP | STAR21B | 1 mg | C, E, P, RE | STAR21B | |
| RPE | STAR20A | 100 TESTS | F | STAR20A | |
| RPE | STAR73 | 100 TESTS | F | STAR73 |
Recommended Negative Isotype Control
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Purified | MCA1212 | 100 TESTS | C, E, F | MCA1212 |
More Reagents With This Specificity
DECTIN-2 Antibody More Images
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DECTIN-2 Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry | 1/50 - 1/100 | |||
| Immunohistology - Frozen | ||||
| Immunohistology - Paraffin | ||||
| Immunoprecipitation | ||||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
DECTIN-2 Antibody References
References
1. Taylor, P.R. et al. (2005) Dectin-2 is predominantly myeloid restricted and exhibits unique activation-dependent expression on maturing inflammatory monocytes elicited in vivo.Eur J Immunol.35:2163 - 2174.
2. Barrett, N.A. et al. (2011) Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes.J Exp Med 28 Feb [epub ahead of print]
2. Suram, S. et al. (2010) Pathways regulating cytosolic phospholipase A2 activation and eicosanoid production in macrophages by Candida albicans.J Biol Chem. 2010 Jul 18. [Epub ahead of print]
4. Robinson, M. et al. (2009) Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection.J Exp Med. 206:2037-51.
DECTIN-2 Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
GO Terms
UniProt
Health And Safety Information
Material Safety Datasheet Documentation #10040 available at:
http://www.abdserotec.com/uploads/MSDS/10040.pdf
http://www.abdserotec.com/uploads/MSDS/10040.pdf
Regulatory
For research purposes only
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