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- CD68 Antibody
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Product CodeMCA1957PEB
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DescriptionRAT ANTI MOUSE CD68:RPE
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Product TypeMonoclonal Antibody
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CloneFA-11
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IsotypeIgG2a
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FormatRPE
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ApplicationsF *
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Pack Size500 TESTS
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
CD68 Antibody Product Summary
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Specificity
Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeabilisation. MCA1957PEB does detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
| 0.09% | Sodium Azide |
| 1% | Bovine Serum Albumin |
| 5% | Sucrose |
Reconstitution
Reconstitute with 5.0 ml distilled water
Immunogen
Purified Concanavalin A acceptor glycoprotein from P815 cell line
Buffer Solution
Phosphate buffered saline
CD68 Antibody Additional Formats
Format Product Code Pack Size Applications List Price Quantity ALEXA FLUOR® 488 MCA1957A488 100 TESTS/1ml F * MCA1957A488ALEXA FLUOR® 488 MCA1957A488T 25 TESTS/0.25ml F * MCA1957A488TALEXA FLUOR® 647 MCA1957A647 100 TESTS/1ml F * MCA1957A647ALEXA FLUOR® 647 MCA1957A647T 25 TESTS/0.25ml F * MCA1957A647TALEXA FLUOR® 700 MCA1957A700 100 TESTS/1ml F * MCA1957A700ALEXA FLUOR® 700 MCA1957A700T 25 TESTS/0.25ml F * MCA1957A700TBiotin MCA1957B 0.1 mg F * MCA1957BBiotin MCA1957BT 25 µg F * MCA1957BTFITC MCA1957F 0.1 mg F * MCA1957FFITC MCA1957FB 0.5 mg F * MCA1957FBFITC MCA1957FT 25 µg F * MCA1957FTFITC MCA1957FA 50 µg F * MCA1957FALow Endotoxin MCA1957EL 0.5 mg C, F *, IF, IP, P *, WB* MCA1957ELPurified MCA1957GA 0.1 mg C, F *, IF, IP, P *, WB* MCA1957GAPurified MCA1957 0.25 mg C, F *, IF, IP, P *, WB* MCA1957Purified MCA1957T 25 µg C, F *, IA, IF, IP, WB* MCA1957TRPE MCA1957PE 100 TESTS F * MCA1957PERPE MCA1957PET 25 TESTS F * MCA1957PET
CD68 Antibody Related Reagents
Recommended Negative Isotype Control
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| RPE | MCA1212PE | 100 TESTS | F | MCA1212PE |
More Reagents With This Specificity
CD68 Antibody More Images
CD68 Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| Flow Cytometry(1) | Neat - 1/10 |
(1)
Membrane permeabilisation is required for this application. Serotec recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041)
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041)
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Recommended protocols are available Here
CD68 Antibody References
References
1. Ramprasad, M.P. et al. (1996) Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein.Proc. Natl. Acad. Sci. 93: 14833-14838.
2. Rabinowitz, S.S. & Gordon, S. (1991) Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli. J. Exp. Med. 174: 827-836.
3. Da Silva, R.P & Gordon, S. (1999) Phagocytosis stimulates alternative glycosylation of macrosialin (mouse CD68), a macrophage-specific endosomal protein. Biochem. J. 338: 687-694.
4. Schleicher, U. et al. (2005) Minute numbers of contaminant CD8+ T cells or CD11b+CD11c+ NK cells are the source of IFN-{gamma} in IL-12/IL-18-stimulated mouse macrophage populations.Blood 105: 1319-1328.
5. von Lukowicz, T. et al. (2008) PARP1 is required for adhesion molecule expression in atherogenesis.Cardiovasc. Res. 78: 158-166
6. Kassim, S. et al. (2010) Gene therapy in a humanized mouse model of familial hypercholesterolemia leads to marked regression of atherosclerosis.PloS ONE 5: e13424.
7. Rahaman, S.O. et al. (2011) Vav family Rho guanine nucleotide exchange factors regulate CD36-mediated macrophage foam cell formation.J Biol Chem. Jan 5. [Epub ahead of print]
8. Frossard, J.L. et al. (2011) Role of CCL-2, CCR-2 and CCR-4 in cerulein-induced acute pancreatitis and pancreatitis-associated lung injury.J Clin Pathol. Feb 23. [Epub ahead of print]
9. West, E.L. et al. (2010) Long-term survival of photoreceptors transplanted into the adult murine neural retina requires immune modulation.Stem Cells. 28: 1997-2007.
10. Lopez, M.E. et al. (2011) Anatomically defined neuron-based rescue of neurodegenerative niemann-pick type C disorder.J Neurosci. 31: 4367-78.
11. Jayagopal, A. et al. (2009) Quantum dot mediated imaging of atherosclerosis.Nanotechnology. 20: 165102.
12. Leung, V.W. et al. (2009) Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice.Am J Pathol. 175: 1757-67.
13. Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.Mol Ther. 17: 65-72.
14. Lu, W. et al. (2010) Photoacoustic imaging of living mouse brain vasculature using hollow gold nanospheres.Biomaterials. 31: 2617-26.
15. de Beer, M.C. et al. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism.J Lipid Res. 44: 674-85.
16. Song, L. et al. (2011) Deletion of the murine scavenger receptor CD68.J Lipid Res. 52: 1542-50.
17. Daldrup-Link, H.E. et al. (2011) MR Imaging of Tumor Associated Macrophages with Clinically-Applicable Iron Oxide Nanoparticles.Clin Cancer Res. Jul 26. [Epub ahead of print]
18. Macauley, S.L. et al. (2011) The Role of Attenuated Astrocyte Activation in Infantile Neuronal Ceroid Lipofuscinosis J. Neurosci 31: 15575-85
19. Martin-Manso, G. et al. (2008) Thrombospondin 1 promotes tumor macrophage recruitment and enhances tumor cell cytotoxicity of differentiated U937 cells.Cancer Res. 68: 7090-9.
20. Lazarini, F. et al. (2012) Early Activation of Microglia Triggers Long-Lasting Impairment of Adult Neurogenesis in the Olfactory Bulb J Neurosci 32: 3652-64.
21. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.J Immunol. 182: 1278-86.
22. Akbarshahi, H. et al. (2012) Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.PLoS One. 7: e42654.
23. Smith, M. and Koch, G. (1987) Differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes.J Cell Sci. 87:113-9.
CD68 Antibody More Information
Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
GO Terms
UniProt
Health And Safety Information
Material Safety Datasheet Documentation #10075 available at:
http://www.abdserotec.com/uploads/MSDS/10075.pdf
http://www.abdserotec.com/uploads/MSDS/10075.pdf
Regulatory
For research purposes only
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