VIAL 1, Serum Block
1 x 15ml

VIAL 2, Reagent Boost
1 x 15ml

VIAL 3, HRP Polymer
1 x 15ml

AbD Serotec HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of AbD Serotec ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete AbD Serotec PDF datasheet, that can be accessed by clicking on the "Download PDF" link above. The procedure has been briefly summarized below:

1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
3) Apply serum block and incubate for 15 minutes.
4) Apply primary antibody and incubate for 30 minutes.
5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
[Discard unused substrate solution]
8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted.

6 months from the date of despatch.

1) Primary antibody and positive control
2) Phosphate Buffered Saline (PBS), pH7.4 (+ 0.2)
3) Hydrogen Peroxide Blocking Reagent
4) Substrate Chromogen (DAB or AEC)
5) Substrate Buffer
6) Counterstain
7) Mounting media

AbD Serotec can supply a number of these products separately, please see the ‘Recommended Useful Reagents’ section.

Instructions for use can be found at www.abdserotec.com/uploads/IFU/STAR3000B.pdf

Material Safety Datasheet documentation #10362; #10282;#10456; available at
http://www.abdserotec.com/uploads/MSDS/10362.pdf
http://www.abdserotec.com/uploads/MSDS/10282.pdf
http://www.abdserotec.com/uploads/MSDS/10456.pdf

For research purposes only