The following buffer solutions are recommended:for preparing the antibody:

10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

It is recommended that 100-150ug of antibody be used for every 100ug APC-Cy7, corresponding to a 1:1 antibody: APC-Cy7 molar ratio. For optimal results the antibody volume should be around 40ul although up to 80ul is acceptable where the antibody concentration is low.

3 Vials of 100ug LYNX lyophilized APC-Cy7 mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent

LNK132APCCY7 enables the rapid conjugation of a pre-prepared lyophilized mixture containing Allophycocyanin (APC)-Cy7 label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of APC-Cy7 to the antibody.

The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.

Click here for further information on our LYNX Rapid Conjugation kits®.

Click here for frequently asked questions about our LYNX Rapid Conjugation kits®.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.

We recommend that for each conjugation the user determines the best antibody:conjugate ratio.

Store kit at -20^oC only.
Newly-conjugated antibody can be stored at 4^oC. For long term storage however, the addition of a preservative is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing.

12 months from date of despatch.

These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting AbD Serotec, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail: sales@abdserotec.com

1.To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

2.Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

3.Replace cap onto vial and incubate in the dark at room temperature (20-25^oC) for 3 hours, or overnight if preferred.

4.After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

Material Safety Datasheet documentation #10553; #10546; #10549 available at
http://www.abdserotec.com/uploads/MSDS/10553.pdf
http://www.abdserotec.com/uploads/MSDS/10546.pdf
http://www.abdserotec.com/uploads/MSDS/10549.pdf

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

For research purposes only