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MHC CLASS II RT1D Antibody
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Product CodeMCA50GA
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DescriptionMouse Anti Rat Mhc Class II RT1D
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Product TypeMonoclonal Antibody
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CloneOX-17
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IsotypeIgG1
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FormatPurified
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ApplicationsC, F
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Pack Size0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
MHC CLASS II RT1D Antibody Product Summary
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Specificity
MCA50GA recognises a monomorphic determinant on rat RT1D, the rat homologue of mouse I-E, present on all rat strains. MRC OX-17 does not cross-react with rat RT1B or mouse I-E antigen, see Fukumoto, T. et al. for details.
This product is routinely tested in flow cytometry on rat splenocytes.
This product is routinely tested in flow cytometry on rat splenocytes.
Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilisers
| 0.09% | Sodium Azide |
Immunogen
Rat spleen glycoproteins
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
Fusion Partners
Spleen cells from immunised mice were fused with cells from the mouse X63.Ag8.653 myeloma cell line .
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
MHC CLASS II RT1D Antibody Additional Formats
MHC CLASS II RT1D Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Alk. Phos. | STAR117A | 0.5 mg | E, WB | STAR117A | |
| Alk. Phos. | STAR87A | 1 mg | C, E, WB | STAR87A | |
| Biotin | AAC10B | 0.5 mg | C, E, P, WB | AAC10B | |
| DyLight®800 | STAR117D800GA | 0.1 mg | F, IF, WB | STAR117D800GA | |
| DyLight®800 | STAR8D800GA | 0.1 mg | F, IF, WB | STAR8D800GA | |
| FITC | STAR117F | 0.5 mg | F | STAR117F | |
| FITC | STAR70 | 0.5 mg | F | STAR70 | |
| FITC | AAC10F | 1 mg | C | AAC10F | |
| FITC | STAR120F | 1 mg | C, F | STAR120F | |
| FITC | STAR9B | 1 mg | F | STAR9B | |
| HRP | HCA036P | 0.1 mg | E | HCA036P | |
| HRP | STAR117P | 0.5 mg | E, WB | STAR117P | |
| HRP | STAR77 | 0.5 mg | C, E, P | STAR77 | |
| HRP | STAR120P | 1 mg | E, WB | STAR120P | |
| HRP | STAR13B | 1 mg | C, E, P, RE, WB | STAR13B | |
| HRP | STAR87P | 1 mg | E | STAR87P | |
| RPE | STAR12A | 100 TESTS | F | STAR12A | |
| RPE | STAR76 | 100 TESTS | F | STAR76 |
Recommended Negative Isotype Control
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Purified | MCA1209 | 0.1 mg | F | MCA1209 |
More Reagents With This Specificity
MHC CLASS II RT1D Antibody More Images
MHC CLASS II RT1D Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry | 1/100 | |||
| Immunohistology - Frozen | ||||
| Immunohistology - Paraffin | ||||
| Immunoprecipitation | ||||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
MHC CLASS II RT1D Antibody References
References
1. Fukumoto, T. et al. (1982) Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.Eur. J. Immunol. 12: 237-243.
2. Romaniuk, A. et al. (1995) Rejection of cartilage formed by transplanted allogeneic chondrocytes: evaluation with monoclonal antibodies.Transplant. Immunol 3: 251-257.
3. Volovitz, I. et al. (2011) Split immunity: immune inhibition of rat gliomas by subcutaneous exposure to unmodified live tumor cells.J Immunol. 187: 5452-62.
4. Zeinstra, E. et al. (2006) Simvastatin inhibits interferon-gamma-induced MHC class II up-regulation in cultured astrocytes.J Neuroinflammation. 3:16.
5. Milicevic, N.M. et al. (2005) T cells are required for the peripheral phase of B-cell maturation.Immunology. 116: 308-17.
6. Wildner, G. and Diedrichs-Möhring, M. (2003) Autoimmune uveitis induced by molecular mimicry of peptides from rotavirus, bovine casein and retinal S-antigen.Eur J Immunol. 33: 2577-87.
7. Sawa, K. and Mochizuki, M. (1997) Effects of bucillamine and antigen-presenting cells in experimental autoimmune uveitis in rats.Jpn J Ophthalmol. 41: 388-95.
8. Gurbuxani, S. et al. (2001) Selective depletion of inducible HSP70 enhances immunogenicity of rat colon cancer cells.Oncogene. 20: 7478-85.
9. Volovitz, I. et al. (2010) T cell vaccination induces the elimination of EAE effector T cells: analysis using GFP-transduced, encephalitogenic T cells.J Autoimmun. 35: 135-44.
10. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.J Neuroimmunol. 209: 16-25.
11. Ghiringhelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferatioJ Exp Med. 202: 919-29.
MHC CLASS II RT1D Antibody More Information
Health And Safety Information
Material Safety Datasheet Documentation #10040 available at:
http://www.abdserotec.com/uploads/MSDS/10040.pdf
http://www.abdserotec.com/uploads/MSDS/10040.pdf
Regulatory
For research purposes only
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