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- Human p53 Antibody
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Product Code7045-3076
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DescriptionMOUSE ANTI p53 MUTANT
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Product TypeMonoclonal Antibody
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Clone240
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IsotypeIgG1
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FormatPurified
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ApplicationsC
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Pack Size0.2 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
Human p53 Antibody Product Summary
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Specificity
7045-3076 reacts with 50 kD nuclear p53 (mutant forms) as expressed in some carcinomas from sites such as breast (30% of tumours), colon (50% of tumours) and lung (70% of tumours).
Target Species
Human
Species Cross-Reactivity
| Target Species | Cross Reactivity |
|---|---|
| Bovine | Yes |
| Chicken | Yes |
| Hamster | Yes |
| Monkey | Yes |
| Mouse | Yes |
| Rat | Yes |
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers
| 0.09% | Sodium Azide (NaN3) |
| 1% | Bovine Serum Albumin |
Immunogen
Murine p53 beta galactosidase fusion protein, expressed in E. coli
Approx. Protein Concentrations
1.0 mg/ml
Buffer Solution
Phosphate buffered saline, pH 7.2
Human p53 Antibody Additional Formats
Human p53 Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Alk. Phos. | STAR117A | 0.5 mg | E, WB | STAR117A Please inquire | |
| Biotin | AAC10B | 0.5 mg | C, E, P, WB | AAC10B Please inquire | |
| DyLight®488 | STAR117D488GA | 0.1 mg | F, IF | STAR117D488GA Please inquire | |
| DyLight®549 | STAR117D549GA | 0.1 mg | F, IF | STAR117D549GA Please inquire | |
| DyLight®649 | STAR117D649GA | 0.1 mg | F, IF | STAR117D649GA Please inquire | |
| DyLight®800 | STAR117D800GA | 0.1 mg | F, IF, WB | STAR117D800GA Please inquire | |
| FITC | STAR120F | 1 mg | C, F | STAR120F Please inquire | |
| FITC | STAR117F | 0.5 mg | F | STAR117F Please inquire | |
| FITC | AAC10F | 1 mg | C | AAC10F Please inquire | |
| HRP | STAR117P | 0.5 mg | E, WB | STAR117P Please inquire | |
| HRP | STAR120P | 1 mg | E, WB | STAR120P Please inquire | |
| HRP | STAR13B | 1 mg | C, E, P, RE, WB | STAR13B Please inquire | |
| HRP | HCA036P | 0.1 mg | E | HCA036P Please inquire | |
| HRP | STAR77 | 0.5 mg | C, E, P | STAR77 Please inquire | |
| RPE | STAR76 | 100 TESTS | F | STAR76 Please inquire |
More Reagents With This Specificity
Human p53 Antibody More Images
Human p53 Antibody Application Notes
Application
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry | ||||
| Immunohistology - Frozen | 1/50 - 1/200 | |||
| Immunohistology - Paraffin | ||||
| Immunohistology - Resin | ||||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
Reactive with methacarn fixed tisues
Human p53 Antibody References
References
1. Purdie, C. A. et al (1991) p53 expression in colorectal tumors.Am. J. Pathol. 138(4): 807-813.
1. Iggo, R. et al (1990) Increased expression of mutant forms of p53 oncogene in primary lung cancer.Lancet 335(8691): 675-679.
1. Banks, L. et al (1986) Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression.Eur. J. Biochem. 159(3): 529-534.
1. Lane, D. P. And Benchimol, S. (1990) p53: oncogene or anti-oncogene?Genes Dev. 4(1): 1-8.
Human p53 Antibody More Information
Contact Us & Support
Events
University of California San Fran-Mission Bay, CA February 29, 2012
Oregon Health & Science University Portland, OR March 14, 2012
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