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- Human p53 Antibody
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Product CodeAHP535
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DescriptionRABBIT ANTI HUMAN p53
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Product TypePolyclonal Antibody
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IsotypePolyclonal IgG
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FormatConcentrate
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ApplicationsC, P *
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Pack Size1 ml
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
Human p53 Antibody Product Summary
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Specificity
AHP535 recognizes both the wild type and mutant forms of p53. The oncoprotein p53 is an intensively studied tumour suppressor protein, which has a vital role in the control of cell growth.
In normal circumstances cells arrest during the G1/G2 phase of the cell cycle, and undertake any DNA repair processes that are necessary. This arrest is mediated by p53, and if p53 is absent or damaged cells with cancerous potential may be able to continue through the cell cycle.
Target Species
Human
Product Form
Serum - liquid, diluted
Preservative Stabilisers
| 0.09% | Sodium Azide |
| 1% | Bovine Serum Albumin |
Immunogen
Recombinant human p53
Buffer Solution
Phosphate buffered saline
Human p53 Antibody Additional Formats
Format Product Code Pack Size Applications List Price Quantity Concentrate AHP535T 0.1 ml C, P * AHP535TPlease inquireReady To Use AHP535H 6 ml C, P * AHP535HPlease inquire- Suggest a new format
Human p53 Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Biotin | 2AB02B | 1 ml | C, E, P, WB | 2AB02B Please inquire | |
| Biotin | STAR121B | 1 mg | E, WB | STAR121B Please inquire | |
| DyLight®488 | STAR36D488GA | 0.1 mg | F, IF | STAR36D488GA Please inquire | |
| DyLight®549 | STAR36D549GA | 0.1 mg | F, IF | STAR36D549GA Please inquire | |
| DyLight®649 | STAR36D649GA | 0.1 mg | F, IF | STAR36D649GA Please inquire | |
| DyLight®800 | STAR36D800GA | 0.1 mg | F, IF, WB | STAR36D800GA Please inquire | |
| FITC | STAR121F | 1 mg | F | STAR121F Please inquire | |
| FITC | STAR34B | 1 mg | C, F | STAR34B Please inquire | |
| HRP | STAR54 | 1 mg | C, E, WB | STAR54 Please inquire | |
| HRP | STAR121P | 1 mg | E, WB | STAR121P Please inquire | |
| HRP | STAR124P | 1 mg | C, E, WB | STAR124P Please inquire | |
| RPE | STAR35A | 100 TESTS | F | STAR35A Please inquire |
Histology Positive Control Tissue
Colon or breast carcinoma
More Reagents With This Specificity
Human p53 Antibody More Images
Human p53 Antibody Application Notes
Application
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry | ||||
| Immunohistology - Frozen | 1/10 - 1/40 | |||
| Immunohistology - Paraffin(1) | 1/10 - 1/40 | |||
| Immunohistology - Resin | ||||
| Immunoprecipitation | ||||
| Radioimmunoassays | ||||
| Western Blotting |
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Human p53 Antibody References
Human p53 Antibody More Information
Storage
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human p53 were raised by repeated immunisation of rabbits with highly purified antigen.
Contact Us & Support
Events
University of California San Fran-Mission Bay, CA February 29, 2012
Oregon Health & Science University Portland, OR March 14, 2012
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