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- Human p53 Antibody
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Product CodeAHP906
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DescriptionRABBIT ANTI p53 (pSer392)
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Product TypePolyclonal Antibody
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IsotypePolyclonal IgG
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FormatPurified
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ApplicationsWB*
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Pack Size0.1 ml
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
Human p53 Antibody Product Summary
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Specificity
AHP906 is specific for p53, when phosphorylated at serine 392.
The tumour suppressor protein p53, is expressed ubiquitously and plays an important role in blocking proliferation of damaged cells.
Phosphorylation of p53 at serine 392 is reported in response to diverse stresses, for example UV irradiation.
Target Species
Human
Species Cross-Reactivity
| Target Species | Cross Reactivity |
|---|---|
| Monkey | Expected from Sequence |
| Rat | Yes |
Product Form
Purified IgG - liquid
Preservative Stabilisers
| 0.09% | Sodium Azide |
| 0.01% | Bovine Serum Albumin |
| 50% | Glycerol |
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within p53 which includes phosphorylated Ser392.
Buffer Solution
10mM HEPES pH7.5
Human p53 Antibody Additional Formats
Human p53 Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Biotin | 2AB02B | 1 ml | C, E, P, WB | 2AB02B Please inquire | |
| Biotin | STAR121B | 1 mg | E, WB | STAR121B Please inquire | |
| DyLight®488 | STAR36D488GA | 0.1 mg | F, IF | STAR36D488GA Please inquire | |
| DyLight®549 | STAR36D549GA | 0.1 mg | F, IF | STAR36D549GA Please inquire | |
| DyLight®649 | STAR36D649GA | 0.1 mg | F, IF | STAR36D649GA Please inquire | |
| DyLight®800 | STAR36D800GA | 0.1 mg | F, IF, WB | STAR36D800GA Please inquire | |
| FITC | STAR121F | 1 mg | F | STAR121F Please inquire | |
| FITC | STAR34B | 1 mg | C, F | STAR34B Please inquire | |
| HRP | STAR54 | 1 mg | C, E, WB | STAR54 Please inquire | |
| HRP | STAR121P | 1 mg | E, WB | STAR121P Please inquire | |
| HRP | STAR124P | 1 mg | C, E, WB | STAR124P Please inquire | |
| RPE | STAR35A | 100 TESTS | F | STAR35A Please inquire |
More Reagents With This Specificity
Human p53 Antibody More Images
Human p53 Antibody Application Notes
Application
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry | ||||
| Immunohistology - Frozen | ||||
| Immunohistology - Paraffin | ||||
| Immunohistology - Resin | ||||
| Immunoprecipitation | ||||
| Western Blotting(1) | 1/1000 |
(1)
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP906 recognises a band of approximately 53kD in rat brain nuclear fraction lysates.
Human p53 Antibody References
References
1. Jaiswal, J. et al. (2001) p53-dependent transcriptional regulation of the APC promoter in colon cancer cells treated with DNA alkylating agents. J. Biol. Chem. 276: 18193-18199.
Human p53 Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to human phosphorylated p53 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Contact Us & Support
Events
University of California San Fran-Mission Bay, CA February 29, 2012
Oregon Health & Science University Portland, OR March 14, 2012
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