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- Human p53 Antibody
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Product CodeMCA1842
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DescriptionMOUSE ANTI HUMAN p53 (pSer392)
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Product TypeMonoclonal Antibody
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CloneFP3-2
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IsotypeIgG1
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FormatPurified
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ApplicationsIP, P *, WB*
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Pack Size0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
Human p53 Antibody Product Summary
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Specificity
MCA1842 recognises human p53, specifically binding only to p53 phosphorylated at the Ser392 residue.
Ser392 phosphorylation of p53 has been shown to occur following DNA damage. Phosphorylation of p53 at this site may be mediated the casein kinase 2 (CK2), and also be other phosphorylation mechanisms.
In immunohistology this antibody generally stains 5-10% of cells labelled with antibodies specific for non-phosphorylated p53.
Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilisers
| 0.09% | Sodium Azide |
Immunogen
Synthetic peptide RKKLMFKTEGPDS[P]D
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
Human p53 Antibody Additional Formats
Human p53 Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Alk. Phos. | STAR117A | 0.5 mg | E, WB | STAR117A Please inquire | |
| Alk. Phos. | STAR87A | 1 mg | C, E, WB | STAR87A Please inquire | |
| Biotin | AAC10B | 0.5 mg | C, E, P, WB | AAC10B Please inquire | |
| DyLight®488 | STAR117D488GA | 0.1 mg | F, IF | STAR117D488GA Please inquire | |
| DyLight®549 | STAR117D549GA | 0.1 mg | F, IF | STAR117D549GA Please inquire | |
| DyLight®649 | STAR117D649GA | 0.1 mg | F, IF | STAR117D649GA Please inquire | |
| DyLight®800 | STAR117D800GA | 0.1 mg | F, IF, WB | STAR117D800GA Please inquire | |
| DyLight®800 | STAR8D800GA | 0.1 mg | F, IF, WB | STAR8D800GA Please inquire | |
| FITC | STAR9B | 1 mg | F | STAR9B Please inquire | |
| FITC | STAR70 | 0.5 mg | F | STAR70 Please inquire | |
| FITC | STAR120F | 1 mg | C, F | STAR120F Please inquire | |
| FITC | STAR117F | 0.5 mg | F | STAR117F Please inquire | |
| FITC | AAC10F | 1 mg | C | AAC10F Please inquire | |
| HRP | STAR117P | 0.5 mg | E, WB | STAR117P Please inquire | |
| HRP | STAR120P | 1 mg | E, WB | STAR120P Please inquire | |
| HRP | STAR87P | 1 mg | E | STAR87P Please inquire | |
| HRP | STAR13B | 1 mg | C, E, P, RE, WB | STAR13B Please inquire | |
| HRP | HCA036P | 0.1 mg | E | HCA036P Please inquire | |
| HRP | STAR77 | 0.5 mg | C, E, P | STAR77 Please inquire | |
| RPE | STAR12A | 100 TESTS | F | STAR12A Please inquire | |
| RPE | STAR76 | 100 TESTS | F | STAR76 Please inquire |
Histology Positive Control Tissue
Colon or breast carcinoma
More Reagents With This Specificity
Human p53 Antibody More Images
Human p53 Antibody Application Notes
Application
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry | ||||
| Immunohistology - Frozen | ||||
| Immunohistology - Paraffin(1) | ||||
| Immunohistology - Resin | ||||
| Immunoprecipitation | ||||
| Radioimmunoassays | ||||
| Western Blotting(2) |
(2)
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Human p53 Antibody References
References
1. Blaydes, J.P. and Hupp, T.R. (1998) DNA damage triggers DRB-resistant phosphorylation of human p53 at the CK2 site.Oncogene 17: 1045-1052.
Human p53 Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of a mouse SP-2 myeloma cell line
UniProt
P53_HUMAN
Health And Safety Information
Material Safety Datasheet Documentation #10040 available at:
http://www.abdserotec.com/uploads/MSDS/10040.pdf
Contact Us & Support
Events
University of California San Fran-Mission Bay, CA February 29, 2012
Oregon Health & Science University Portland, OR March 14, 2012
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