Product Images

  • Immunofluorescence showing p53 in A-431cells using Mouse anti Human p53:Azide Free (MCA3510Z)
  • Staining of paraffin embedded human salivary gland with Mouse anti Human p53:Azide Free (MCA3510Z)
  • Western blot analysis of p53 expression in A-431 using Mouse anti Human p53:Azide Free (MCA3510Z)

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List Price Quantity
MCA3510Z
Please inquire
  • Human p53 Antibody
  • Product Code
    MCA3510Z
  • Description
    MOUSE ANTI HUMAN p53:Azide Free
  • Product Type
    Monoclonal Antibody
  • Clone
    2C3
  • Isotype
    IgG1
  • Format
    Azide Free
  • Applications
    IF, P *, WB
  • Pack Size
    0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

Human p53 Antibody Product Summary

Specificity

MCA3510Z is specific for human p53.

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preservative Stabilisers

None present

Immunogen

Recombinant protein corresponding to aa 94 - 202 of human TP53

Approx. Protein Concentrations

Ig concentration 0.5 mg/ml

Buffer Solution

Phosphate buffered saline

Human p53 Antibody Additional Formats

Human p53 Antibody Related Reagents

Recommended Secondary Antibody

FormatProduct CodePack SizeApplicationsList PriceQuantity
Alk. Phos.STAR117A0.5 mgE, WB
STAR117A
Please inquire
Alk. Phos.STAR87A1 mgC, E, WB
STAR87A
Please inquire
BiotinAAC10B0.5 mgC, E, P, WB
AAC10B
Please inquire
DyLight®488STAR117D488GA0.1 mgF, IF
STAR117D488GA
Please inquire
DyLight®549STAR117D549GA0.1 mgF, IF
STAR117D549GA
Please inquire
DyLight®649STAR117D649GA0.1 mgF, IF
STAR117D649GA
Please inquire
DyLight®800STAR117D800GA0.1 mgF, IF, WB
STAR117D800GA
Please inquire
DyLight®800STAR8D800GA0.1 mgF, IF, WB
STAR8D800GA
Please inquire
FITCSTAR9B1 mgF
STAR9B
Please inquire
FITCSTAR700.5 mgF
STAR70
Please inquire
FITCSTAR120F1 mgC, F
STAR120F
Please inquire
FITCSTAR117F0.5 mgF
STAR117F
Please inquire
FITCAAC10F1 mgC
AAC10F
Please inquire
HRPSTAR117P0.5 mgE, WB
STAR117P
Please inquire
HRPSTAR120P1 mgE, WB
STAR120P
Please inquire
HRPSTAR87P1 mgE
STAR87P
Please inquire
HRPSTAR13B1 mgC, E, P, RE, WB
STAR13B
Please inquire
HRPHCA036P0.1 mgE
HCA036P
Please inquire
HRPSTAR770.5 mgC, E, P
STAR77
Please inquire
RPESTAR12A100 TESTSF
STAR12A
Please inquire
RPESTAR76100 TESTSF
STAR76
Please inquire

More Reagents With This Specificity

Products with "p53" specificity

Human p53 Antibody More Images

    • Product Images

    • Immunofluorescence showing p53 in A-431cells using Mouse anti Human p53:Azide Free (MCA3510Z)
    • Staining of paraffin embedded human salivary gland with Mouse anti Human p53:Azide Free (MCA3510Z)
    • Western blot analysis of p53 expression in A-431 using Mouse anti Human p53:Azide Free (MCA3510Z)

Human p53 Antibody Application Notes

Application

Application NameYesNoNot DeterminedSuggested Dilution
Immunofluorescence0.1 - 10 ug/ml
Immunohistology - Paraffin(1)0.1 - 10 ug/ml
Western Blotting

(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Human p53 Antibody References

Human p53 Antibody More Information

Storage

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Fusion Partners

Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

UniProt

P53_HUMAN

Health And Safety Information

Material Safety Datasheet Documentation #10162 available at:
http://www.abdserotec.com/uploads/MSDS/10162.pdf

Events

University of California San Fran-Mission Bay, CA   February 29, 2012
Oregon Health & Science University Portland, OR  March 14, 2012