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- Human p53 Antibody
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Product CodeMCA909
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DescriptionMOUSE ANTI p53 (aa213-217)
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Product TypeMonoclonal Antibody
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ClonePAb240
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IsotypeIgG1
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FormatPurified
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ApplicationsIP, WB*
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Pack Size0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
Human p53 Antibody Product Summary
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Specificity
MCA909 recognises an epitope of p53 between amino acids 156-214 which is present in some malignant tissues but not in normal and pre-malignant tissues. PAb240 is commonly reported as being mutant specific, however there are reports in the literature which suggest that conformational changes in the wild type may result in recognition by this clone.
Target Species
Human
Species Cross-Reactivity
| Target Species | Cross Reactivity |
|---|---|
| Bovine | Yes |
| Chicken | Yes |
| Hamster | Yes |
| Mammals | Yes |
| Monkey | Yes |
| Mouse | Yes |
| Rat | Yes |
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
p53-beta-galactosidase fusion protein
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Buffer Solution
Phosphate buffered saline
Human p53 Antibody Additional Formats
Human p53 Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Alk. Phos. | STAR117A | 0.5 mg | E, WB | STAR117A Please inquire | |
| Alk. Phos. | STAR87A | 1 mg | C, E, WB | STAR87A Please inquire | |
| Biotin | AAC10B | 0.5 mg | C, E, P, WB | AAC10B Please inquire | |
| DyLight®488 | STAR117D488GA | 0.1 mg | F, IF | STAR117D488GA Please inquire | |
| DyLight®549 | STAR117D549GA | 0.1 mg | F, IF | STAR117D549GA Please inquire | |
| DyLight®649 | STAR117D649GA | 0.1 mg | F, IF | STAR117D649GA Please inquire | |
| DyLight®800 | STAR117D800GA | 0.1 mg | F, IF, WB | STAR117D800GA Please inquire | |
| DyLight®800 | STAR8D800GA | 0.1 mg | F, IF, WB | STAR8D800GA Please inquire | |
| FITC | STAR9B | 1 mg | F | STAR9B Please inquire | |
| FITC | STAR70 | 0.5 mg | F | STAR70 Please inquire | |
| FITC | STAR120F | 1 mg | C, F | STAR120F Please inquire | |
| FITC | STAR117F | 0.5 mg | F | STAR117F Please inquire | |
| FITC | AAC10F | 1 mg | C | AAC10F Please inquire | |
| HRP | STAR117P | 0.5 mg | E, WB | STAR117P Please inquire | |
| HRP | STAR120P | 1 mg | E, WB | STAR120P Please inquire | |
| HRP | STAR87P | 1 mg | E | STAR87P Please inquire | |
| HRP | STAR13B | 1 mg | C, E, P, RE, WB | STAR13B Please inquire | |
| HRP | HCA036P | 0.1 mg | E | HCA036P Please inquire | |
| HRP | STAR77 | 0.5 mg | C, E, P | STAR77 Please inquire | |
| RPE | STAR12A | 100 TESTS | F | STAR12A Please inquire | |
| RPE | STAR76 | 100 TESTS | F | STAR76 Please inquire |
More Reagents With This Specificity
Human p53 Antibody More Images
Human p53 Antibody Application Notes
Application
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry | ||||
| Immunohistology - Frozen | ||||
| Immunohistology - Paraffin | ||||
| Immunohistology - Resin | ||||
| Immunoprecipitation | ||||
| Western Blotting(1) |
(1)
Non -reducing conditions are required for discrimination between wild and mutant forms of p53 using Pab240.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
* Mutant p53 occurs because point mutations cause a conformational disruption. This exposes an internal epitope that MCA909 is specific for. To be able to distinguish between wild and mutant forms of p53 non reduced conditions must be used.
Human p53 Antibody References
References
1. Gannon, et al. (1990) Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form. EMBO J. 9: 1595-1602.
2. Iggo, et al. (1990) Increased expression of mutant forms of p53 oncogene in primary lung cancer. Lancet. 335: 675-679.
3. Pezzella, F. et al. (1994) Antibody for detecting p53 protein by immunohistochemistry in normal tissues. J. Clin. Pathol. 47: 592 - 596.
4. Legros, Y. et al (1994) Mutations in p53 produce a common conformational effect that can be detected with a panel of monoclonal antibodies directed toward the central part of the p53 protein. Oncogene. 9: 3689-3694.
5. Milner, J. et al. (1990) Temperature-dependent switching between "wild-type" and "mutant" forms of p53-Val135. J Mol Biol. 216: 481-484.
6. Stephen, C.W. et al (1992) Mutant conformation of p53. Precise epitope mapping using a filamentous phage epitope library. J Mol Biol. 225: 577-583.
7. Said, J. W. et al (1992) Immunohistochemical analysis of p53 expression in malignant lymphomas.Am J Pathol. 141(6): 1343-1348.
8. Rivas, C. I. et al (1992) Constitutive expression of p53 protein in enriched normal human marrow blast cell populations.Blood. 79(8): 1982-1986.
9. Kramata, P. et al (2005) Patches of mutant p53-immunoreactive epidermal cells induced by chronic UVB Irradiation harbor the same p53 mutations as squamous cell carcinomas in the skin of hairless SKH-1 mice.Cancer Res. 65(9): 3577-3585.
Human p53 Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch
UniProt
P53_HUMAN
Health And Safety Information
Material Safety Datasheet Documentation #10041 available at:
http://www.abdserotec.com/uploads/MSDS/10041.pdf
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