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- Human p53 Antibody
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Product CodeOBT3175
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DescriptionMOUSE ANTI HUMAN p53 (aa20-25)
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Product TypeMonoclonal Antibody
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CloneBP53-12
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IsotypeIgG2a
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FormatPurified
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ApplicationsC, P *, WB
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Pack Size0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
Human p53 Antibody Product Summary
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Specificity
OBT3175 recognises the human p53 nuclear protein. This clone recognises amino acids 20 - 25, in both wild type and mutant p53.
Target Species
Human
Species Cross-Reactivity
| Target Species | Cross Reactivity |
|---|---|
| Mouse | No |
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilisers
| 0.09% | Sodium Azide |
| 0.1% | Bovine Serum Albumin |
Approx. Protein Concentrations
0.1mg/ml
Buffer Solution
Phosphate buffered saline
Human p53 Antibody Additional Formats
Format Product Code Pack Size Applications List Price Quantity FITC OBT3175F 100 TESTS F * OBT3175FPlease inquire- Suggest a new format
Human p53 Antibody Related Reagents
Recommended Secondary Antibody
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Alk. Phos. | STAR117A | 0.5 mg | E, WB | STAR117A Please inquire | |
| Alk. Phos. | STAR87A | 1 mg | C, E, WB | STAR87A Please inquire | |
| Biotin | AAC10B | 0.5 mg | C, E, P, WB | AAC10B Please inquire | |
| DyLight®488 | STAR117D488GA | 0.1 mg | F, IF | STAR117D488GA Please inquire | |
| DyLight®549 | STAR117D549GA | 0.1 mg | F, IF | STAR117D549GA Please inquire | |
| DyLight®649 | STAR117D649GA | 0.1 mg | F, IF | STAR117D649GA Please inquire | |
| DyLight®800 | STAR117D800GA | 0.1 mg | F, IF, WB | STAR117D800GA Please inquire | |
| DyLight®800 | STAR8D800GA | 0.1 mg | F, IF, WB | STAR8D800GA Please inquire | |
| FITC | STAR9B | 1 mg | F | STAR9B Please inquire | |
| FITC | HCA037F | 0.1 mg | F | HCA037F Please inquire | |
| FITC | STAR70 | 0.5 mg | F | STAR70 Please inquire | |
| FITC | STAR120F | 1 mg | C, F | STAR120F Please inquire | |
| FITC | STAR117F | 0.5 mg | F | STAR117F Please inquire | |
| FITC | AAC10F | 1 mg | C | AAC10F Please inquire | |
| HRP | STAR117P | 0.5 mg | E, WB | STAR117P Please inquire | |
| HRP | STAR120P | 1 mg | E, WB | STAR120P Please inquire | |
| HRP | HCA037P | 0.1 mg | E | HCA037P Please inquire | |
| HRP | STAR87P | 1 mg | E | STAR87P Please inquire | |
| HRP | STAR13B | 1 mg | C, E, P, RE, WB | STAR13B Please inquire | |
| HRP | STAR77 | 0.5 mg | C, E, P | STAR77 Please inquire | |
| RPE | STAR12A | 100 TESTS | F | STAR12A Please inquire | |
| RPE | STAR76 | 100 TESTS | F | STAR76 Please inquire |
Histology Positive Control Tissue
Colon or breast carcinoma
More Reagents With This Specificity
Human p53 Antibody More Images
Human p53 Antibody Application Notes
Application
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry | ||||
| Immunohistology - Frozen | ||||
| Immunohistology - Paraffin(1) | ||||
| Immunohistology - Resin | ||||
| Immunoprecipitation | ||||
| Western Blotting |
(1)
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
OBT3175 recognises the 53kDa nuclear protein under non-reducing conditions
Human p53 Antibody References
References
1. Gannon, J.V. et al. (1990) Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form. EMBO Journal. 9: 1595-1602.
2. Banks, L. et al. (1986) Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression. Eur. J. Biochem. 159: 529-534.
3. Harlow, E. et al. (1981) Monoclonal antibodies specific for simian virus 40 tumor antigensJ. Virol. 39: 861-870.
Human p53 Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
UniProt
P53_HUMAN
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Contact Us & Support
Events
University of California San Fran-Mission Bay, CA February 29, 2012
Oregon Health & Science University Portland, OR March 14, 2012
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