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- Human p53 Antibody
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Product CodeOBT3175F
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DescriptionMOUSE ANTI HUMAN p53 (aa20-25):FITC
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Product TypeMonoclonal Antibody
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CloneBP53-12
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IsotypeIgG2a
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FormatFITC
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ApplicationsF *
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Pack Size100 TESTS
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
Human p53 Antibody Product Summary
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Specificity
OBT3175F recognises the human p53 nuclear protein. This clone recognises amino acids 20 - 25, in both wild type and mutant p53.
Target Species
Human
Species Cross-Reactivity
| Target Species | Cross Reactivity |
|---|---|
| Mouse | No |
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilisers
| 0.09% | Sodium Azide |
| 0.1% | Bovine Serum Albumin |
Buffer Solution
Phosphate buffered saline
Human p53 Antibody Additional Formats
Format Product Code Pack Size Applications List Price Quantity Purified OBT3175 0.1 mg C, P *, WB OBT3175Please inquire- Suggest a new format
Human p53 Antibody Related Reagents
Recommended Negative Isotype Control
| Format | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| FITC | MCA929F | 100 TESTS | F | MCA929F Please inquire |
More Reagents With This Specificity
Human p53 Antibody More Images
Human p53 Antibody Application Notes
Application
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| ELISA | ||||
| Flow Cytometry(1) | Neat | |||
| Immunohistology - Frozen | ||||
| Immunohistology - Paraffin | ||||
| Immunohistology - Resin | ||||
| Immunoprecipitation |
(1)
Cell permeabilisation is required for this application. Serotec recommends the use of Leucoperm™ (Product Code BUF09) for this purpose. (See Worksheet 17 - available on request).
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Recommended protocols are available on request.
Human p53 Antibody References
References
1. Gannon, J.V. et al. (1990) Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form. EMBO Journal. 9: 1595-1602.
2. Banks, L. et al. (1986) Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression. Eur. J. Biochem. 159: 529-534.
3. Harlow, E. et al. (1981) Monoclonal antibodies specific for simian virus 40 tumor antigensJ. Virol. 39: 861-870.
Human p53 Antibody More Information
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of despatch.
UniProt
P53_HUMAN
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Contact Us & Support
Events
University of California San Fran-Mission Bay, CA February 29, 2012
Oregon Health & Science University Portland, OR March 14, 2012
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