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- RAT IgG2a NEGATIVE CONTROL Antibody
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Product CodeMCA1212PE
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DescriptionRAT IgG2a NEGATIVE CONTROL:RPE
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Product TypeNegative/Isotype Control
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IsotypeIgG2a
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FormatRPE
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ApplicationsF
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Pack Size100 TESTS
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information
RAT IgG2a NEGATIVE CONTROL Antibody Product Summary
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Specificity
Negative on all mouse cells and cell lines tested. This antibody recognises a human cell surface marker, and therefore cannot be used as a negative control in this species.
Test results have shown that MCA1212PE is also suitable for use as a negative control with canine tissues.
Test results have shown that MCA1212PE is also suitable for use as a negative control with canine tissues.
Target Species
Negative Control
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
| 0.09% | Sodium Azide |
| 1% | Bovine Serum Albumin |
| 5% | Sucrose |
Reconstitution
Reconstitute with 1 ml distilled water
Immunogen
Human lymphocytes.
Buffer Solution
Phosphate buffered saline
RAT IgG2a NEGATIVE CONTROL Antibody Additional Formats
Format Product Code Pack Size Applications List Price Quantity ALEXA FLUOR® 405 MCA1212A405 100 TESTS/1ml F MCA1212A405ALEXA FLUOR® 488 MCA1212A488 100 TESTS/1ml F MCA1212A488ALEXA FLUOR® 647 MCA1212A647 100 TESTS/1ml F MCA1212A647ALEXA FLUOR® 700 MCA1212A700 100 TESTS/1ml F MCA1212A700Azide Free MCA1212XZ 1 mg C, E, F MCA1212XZBiotin MCA1212B 0.1 mg F MCA1212BFITC MCA1212F 100 TESTS F MCA1212FLow Endotoxin MCA1212EL 0.5 mg F MCA1212ELPacific Blue® MCA1212PB 100 TESTS/1ml F MCA1212PBPurified MCA1212 100 TESTS C, E, F MCA1212RPE-ALEXA FLUOR® 647 MCA1212P647 100 TESTS/1ml F MCA1212P647RPE-CY5 MCA1212C 100 TESTS F MCA1212C
RAT IgG2a NEGATIVE CONTROL Antibody Related Reagents
RAT IgG2a NEGATIVE CONTROL Antibody More Images
Application Name Reference Images Flow Cytometry
RAT IgG2a NEGATIVE CONTROL Antibody Application Notes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
| Application Name | Yes | No | Not Determined | Suggested Dilution |
|---|---|---|---|---|
| Flow Cytometry | * |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
RAT IgG2a NEGATIVE CONTROL Antibody References
References
1. Sumagin, R. et al. (2008) Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling.Am J Physiol Heart Circ Physiol. 295: H969-H977.
2. Chiu, W.C. et al. (2011) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.J Nutr Biochem. 20: 254-60.
3. Guilloteau, L.A. et al. (2003) Nramp1 is not a major determinant in the control of Brucella melitensis infection in mice.Infect Immun. 71: 621-8.
4. Stapleton, T.W. et al. (2000) Investigation of the regenerative capacity of an acellular porcine medial meniscus for tissue engineering applications.Tissue Eng Part A. 17: 231-42.
5. Park, S.W. et al. (2012) A1 adenosine receptor allosteric enhancer PD-81723 protects against renal ischemia-reperfusion injury.Am J Physiol Renal Physiol. 303: F721-32.
6. Schmidt, E.P. et al. (2012) The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.Nat Med. Jul 22. [Epub ahead of print]
7. McConnell, M.J. et al. (2009) H2-K(b) and H2-D(b) regulate cerebellar long-term depression and limit motor learning.Proc Natl Acad Sci U S A. 106: 6784-9.
RAT IgG2a NEGATIVE CONTROL Antibody More Information
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Health And Safety Information
Material Safety Datasheet Documentation #10075 available at:
http://www.abdserotec.com/uploads/MSDS/10075.pdf
http://www.abdserotec.com/uploads/MSDS/10075.pdf
Regulatory
For research purposes only
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