Store at +4oC. DO NOT FREEZE.
This product is photosensitive and should be protected from light.
Avoid exposure to heat and contamination with metal ions or peroxidase.
Store in bottles made of High Density Polyethylene (HDPE).
48 months from date of despatch.
Instructions For Use
1. It is recommended that 100ul of BUF062B TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark.
2. Add 100ul substrate solution per microtiter well.
3. Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil.
4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.
N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF062B is not recommended).
Health And Safety Information
For research purposes only