Ready to use TMB solution - liquid

BUF062B is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA as a substrate for horseradish peroxidase (HRP).

BUF062B contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. The activity of TMB has been optimised to enable increased sensitivity, minimal background and rapid development.

BUF062B produces a deep blue colour during the enzymatic degradation of H₂O₂ by horseradish Peroxidase. The reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.

TMB CORE+ has similar sensitivity to TMB PLUS (BUF042A or BUF042B), but an increased shelf life.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Store at +4^oC. DO NOT FREEZE.
This product is photosensitive and should be protected from light.

Avoid exposure to heat and contamination with metal ions or peroxidase.

Store in bottles made of High Density Polyethylene (HDPE).

48 months from date of despatch.

1. It is recommended that 100ul of BUF062B TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark.

2. Add 100ul substrate solution per microtiter well.

3. Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil.

4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.

N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF062B is not recommended).

Material Safety Datasheet Documentation #10111 available at:
http://www.abdserotec.com/uploads/MSDS/10111.pdf

For research purposes only