Product Images

  • Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium Falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from Plasmodium falciparum) was titrated using  the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium FalciparumHRPII (HCA159) antibody

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HCA159P
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Histidine Rich Protein II Antibody

  • Product Code
    HCA159P
  • Description
    Human anti Plasmodium falciparum HRPII:HRP
  • Product Type
    Monoclonal Antibody
  • Clone
    14964
  • Isotype
    IgG1
  • Format
    HRP
  • Applications
    E
  • Pack Size
    0.1 mg
Product Summary
Additional Formats
Related Reagents
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Application Notes
References
More Information

Specificity

Human anti Plasmodium falciparum HRPII antibody, clone 14964 recognizes HRPII (Histidine Rich Protein II) from the Malaria causing protozoan species Plasmodium falciparum .


Clone 14964 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.

When conjugated this clone can be used as a detection antibody with HCA160 as the capture antibody in sandwich assay.

Target Species

Protozoan

Product Form

Human IgG1 antibody selected from the HuCAL ® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.

Preparation

Purified IgG prepared by affinity chromatography on Protein A.

Preservative Stabilisers

0.01% Thiomersal
HRP Stabiliser (BUF052A)

Immunogen

Recombinant HRPII fusion protein from Plasmodium falciparum

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Buffer Solution

Phosphate buffered saline.

Storage

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.

Shelf Life

12 months from date of despatch.
    • Product Images

    • Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium Falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from Plasmodium falciparum) was titrated using  the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium FalciparumHRPII (HCA159) antibody

Application

Application NameYesNoNot DeterminedSuggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice

Protocols and further information may be found in the HuCAL Technical Manual

ELISA

This product is suitable for use in direct ELISA applications. This product may be used as a detection reagent in a sandwich ELISA together with HCA160 as the capture reagent.

Health And Safety Information

Material Safety Datasheet documentation #10031 available at
http://www.abdserotec.com/uploads/MSDS/10031.pdf

Acknowledgements

Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

Licensed Use

For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.

Regulatory

For research purposes only
Copyright © 2014 Bio-Rad AbD Serotec
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