CD11c Antibody | 8A2

Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD11c:FITC (MCA1441F)

Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD11c:FITC (MCA1441F)

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  • Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD11c:FITC (MCA1441F)
  • Mouse anti Rat CD11c
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  • Product Type
    Monoclonal Antibody
  • Clone
    8A2
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1441GAC, F, IF, IP0.1 mg
    MCA1441GA
    MCA1441FF0.1 mg
    MCA1441F
    MCA1441C, F, IF, IP0.25 mg
    MCA1441
    MCA1441A647F100 Tests/1ml
    MCA1441A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Rat CD11c antibody, clone 8A2 recognizes the rat CD11c cell surface antigen, expressed by some myeloid cells and dendritic cells.

      Mouse anti Rat CD11c antibody, clone 8A2 immunoprecipitates a 130-140/90kDa heterodimer and modestly inhibits macrophage adherence on plastic (by around 20-30%).
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Rat alveolar macrophages
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNo
      Flow Cytometry
    • Application NameYesNo
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNo
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNo
      Flow Cytometry

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      Please view the datasheet pdf for suggested dilutions.

    Additional CD11c Antibody Formats

    FormatsCloneApplicationsSizes available
    CD11c Antibody : Alexa Fluor® 6478A2F100 Tests/1ml
    CD11c Antibody : FITC8A2F0.1 mg
    CD11c Antibody : Purified8A2C, F, IF, IP0.1 mg | 0.25 mg
    • Copyright © 2014 Bio-Rad AbD Serotec

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        MCA1210
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA1210F0.1 mgF
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        Useful Reagents

        Recommended Positive Controls

        Histology Controls

          • Product Images

          • Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD11c:FITC (MCA1441F)
        • Application NameReference Images
          Flow Cytometry
          Immunofluorescence

        References

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