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  • Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD11c:FITC (MCA1441F)

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MCA1441F
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CD11c Antibody

  • Product Code
    MCA1441F
  • Description
    Mouse anti Rat CD11c:FITC
  • Product Type
    Monoclonal Antibody
  • Clone
    8A2
  • Isotype
    IgG2a
  • Format
    FITC
  • Applications
    F
  • Pack Size
    0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

Specificity

Mouse anti Rat CD11c antibody, clone 8A2 recognizes the rat CD11c cell surface antigen, expressed by some myeloid cells and dendritic cells.

Mouse anti Rat CD11c antibody, clone 8A2 immunoprecipitates a 130-140/90kDa heterodimer and modestly inhibits macrophage adherence on plastic (by around 20-30%).

Target Species

Rat

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preservative Stabilisers

0.09%Sodium Azide
1%Bovine Serum Albumin

Immunogen

Rat alveolar macrophages

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Buffer Solution

Phosphate buffered saline

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage

Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.
  • FormatProduct CodePack SizeApplicationsList PriceQuantity
    Alexa Fluor® 647MCA1441A647100 Tests/1mlF
    MCA1441A647
    PurifiedMCA1441GA0.1 mgC, F, IF, IP
    MCA1441GA
    PurifiedMCA14410.25 mgC, F, IF, IP
    MCA1441

Recommended Negative Isotype Control

FormatProduct CodePack SizeApplicationsList PriceQuantity
FITCMCA1210F0.1 mgF
MCA1210F

More Reagents With This Specificity

    • Product Images

    • Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD11c:FITC (MCA1441F)
  • Application NameReference Images
    Flow Cytometry
    Immunofluorescence

Application

Application NameYesNoNot DeterminedSuggested Dilution
Flow CytometryNeat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10ul of the suggested working dilution to label 106 cells in 100ul.

References

1. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J. Neuroimmunol. 209: 16-25.
2. Baba, T. et al . (2008) Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/perforin-dependent mechanism.
J Immunol. 180: 2999-3006.
3. Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
Virology. 388: 78-90.
4. Bode, U. et al. (2008) Dendritic cell subsets in lymph nodes are characterized by the specific draining area and influence the phenotype and fate of primed T cells.
Immunology. 123: 480-90.
5. Veelken, R. et al (2008) Autonomic renal denervation ameliorates experimental glomerulonephritis.
J Am Soc Nephrol. 19: 1371-8.

Health And Safety Information

Material Safety Datasheet Documentation #10041 available at:
http://www.abdserotec.com/uploads/MSDS/10041.pdf

Regulatory

For research purposes only
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