Antibody Conjugation Services
Antibody Conjugation – reproducibility and consistency
High-quality, reproducible antibody conjugation is essential for consistent experimental results. Our proprietary purification processes have been developed to maximize the yield, purity, and immunoreactivity of your antibodies.
- Conjugation of small quantities through to industrial scale large batches.
- Extensive range of markers for conjugation: including alkaline phosphatase, biotin, HRP and the fluorescent dyes summarised below.
| Dye | Laser excitation line (nm) |
Maximal absorbance (nm) | Maximal emission (nm) | Suggested laser | Fluores- cence color |
|---|---|---|---|---|---|
| Alexa Fluor® 405*(1) | 405, 407 | 401 | 421 | Violet diode | |
| Pacific Blue™*†(1) | 405, 407 | 416 | 456 | Violet diode | |
| FITC | 488 | 495 | 525 | Argon-Ion | |
| Alexa Fluor® 488(1) | 488 | 495 | 519 | Argon-Ion | |
| RPE | 488 | 490, 565 | 578 | Argon-Ion | |
| APC | 633, 635, 647 | 650 | 661 | Krypton-Argon | |
| Alexa Fluor® 647(1) | 633, 635, 647 | 650 | 665 | Krypton-Argon | |
| PE-Alexa Fluor® 647#(1) | 488 | 496, 546 | 667 | Argon-Ion | |
| PE-Alexa Fluor® 750(1) | 488 | 496, 546 | 779 | Argon-Ion | |
| DyLight™ 405 | 405 | 400 | 420 | Violet diode | |
| DyLight™ 488 | 488 | 493 | 518 | Argon-Ion | |
| DyLight™ 549 | 550 | 562 | 576 | Krypton-Argon | |
| DyLight™ 649 | 646 | 654 | 673 | Krypton-Argon | |
| DyLight™ 680 | 682 | 680 | 715 | Argon-Ion | |
| DyLight™ 750 | 752 | 750 | 778 | Argon-Ion | |
| DyLight™ 800 | 770 | 777 | 794 | DPSS | |
| Alexa Fluor® 700(1) | 675-700 | 696 | 719 | Xenon-arc |
(1) For conjugation to AbD Serotec primary antibodies only.
* Can only be used on flow cytometers with a violet laser (402–415 nm range).
† Low spectral overlap with green fluorochromes e.g. FITC.
# Caution: if you intend to use PE-Alexa Fluor® 647 in conjunction with APC or Alexa Fluor® 647, please be aware of the possibility of excitation in the tandem dye directly by the 633/635/647 laser.
DPSS is a diode-pumped solid state laser
- Click here for further information about our Alexa Fluor® conjugates
- Click here for further information about our DyLight™ dyes
Alexa Fluor® and Pacific Blue™ are trademarks of Molecular Probes Inc, OR, USA.
DyLight™ is a trademark of Thermo Fisher Scientific and its subsidiaries.
Purification
Our proprietary purification processes are designed to maximize the yield, purity, and immunoreactivity of your antibodies. We offer protein G, protein A and ion exchange chromatography purification processes. The method selected is based on the antibody provided and/or customer requirements.
Quality Control
We test and validate all antibodies according to our ISO 9001-accredited design and production quality assurance system. Antibody purity is assessed by SDS-PAGE, HPLC, or IEF. Our comprehensive testing facility includes ELISA, flow cytometry, immunohistochemistry and Western blot.
Confidentiality
We treat all data and material that you provide us as completely confidential, right down to your name and affiliation. A signed Confidential Disclosure Agreement is available on request.
Customer Satisfaction
We pride ourselves on our flexibility towards customers’ needs and the highest quality pre- and post-production technical support. Our friendly and knowledgeable staff aim to answer all enquiries within a day.
