What is Western Blotting?

Western Blotting

Western blotting, also known as immunoblotting or protein blotting, is a core technique in cell and molecular biology . In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells . 

The Western blotting procedure relies upon three key elements to accomplish this task:

  • the separation of protein mixtures by size using gel electrophoresis;
  • the efficient transfer of separated proteins to a solid support;
  • and the specific detection of a target protein by appropriately matched antibodies.

Once detected, the target protein will be visualized as a band on a blotting membrane, X-ray film, or an imaging system . Since Western blotting is accomplished rapidly, using simple equipment and inexpensive reagents, it is one of the most common laboratory techniques . The results achieved are also easy to interpret, unique, and unambiguous . Therefore, it is routinely used on its own, or along with other immunoassays, in research and clinical settings.


Why use a Western Blot?

Western blots are in wide use across a broad range of scientific and clinical disciplines . Their ability to clearly show the presence of a specific protein both by size and through the binding of an antibody makes them well-suited for evaluating levels of protein expression in cells, and for monitoring fractions during protein purification . Likewise, they are helpful for comparing expression of a target protein from various tissues, or seeing how a particular protein responds to disease or drug treatment .

In many cases, Western blots are used in combination with other key antibody based detection techniques, such as ELISAs or immunohistochemistry.

Western Blotting Antibodies

Antibodies selected for immunodetection should be Western blot tested if possible, with attention paid to the experimental conditions recommended by the antibody supplier . Usually, Western blot positive antibodies recognize a short linear sequence of amino acids found within the target protein that remains intact, or becomes visible, when the target protein is fully unraveled . This is because most Western blots are carried out under denaturing and reducing conditions which remove all higher order protein structure .

In contrast, some epitopes can be conformational, forming a three dimensional structural configuration of amino acids that will be lost upon denaturation of the protein . Thus, not all antibodies work in a typical Western blot . Since Western blot procedures allow for flexibility in choosing gel electrophoresis and blotting conditions, it is possible to modify buffers to retain enough higher order protein structure for detection by some antibodies . 

AbD Serotec is one of the world’s leading manufacturers of primary and secondary antibodies for Western blot anaylsis. In addition, we offer ISO-certified pre-made buffers to simplify immunostaining and make results more consistent from blot to blot. Our 100% Satisfaction Guarantee is backed by our quality, which makes ordering from AbD Serotec totally risk free!


Western Blotting Buffers & Accessories

  • Ordinal Number: 9015
SpecificityFormatTargetHostIsotypeCloneApplications
AP STABILIZING DILUENT antibodyReady To UseC, E, P, WB
BLOCK ACE antibodyReagentE, WB
HORSERADISH PEROXIDASE antibodyReagentE, WB
HRP-STABILIZING DILUENT antibodyReady To UseC, E, P, WB
PROTEIN STABILIZER antibodyReady To UseIY

 

Our range of specialized reagents are designed to significantly improve results in Western blotting:

  • Block Ace This high-performance blocking reagent for Western blotting is superior to 1% BSA. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves. It can also be used as a wash buffer, and for diluting antibodies.
  • HiSpec This assay diluent works to significantly reduce cross-reactivity and non-specific binding in Western blotting, greatly improving your results. HiSpec assay diluent is designed to be used instead of sample buffer or antibody dilution buffer within the Western blotting protocol.
  • AbGuard® HRP Stabilizer Is ideal for use as a diluent for peroxidase conjugated antibodies and other peroxidase conjugated proteins. It provides superior stabilization of HRP-conjugates at low and high dilutions, preventing the loss of peroxidise activity.

Western Blotting Loading Control Antibodies

AbD Serotec offers a range of antibodies to popular loading controls suitable for Western blotting. Our loading control antibodies are offered in a variety of different formats ideal for saving time with direct staining techniques and for use in infrared imaging systems e.g. LI-COR Odyssey®.

Western Blotting Loading Control Antibodies

  • Ordinal Number: 9021
SpecificityFormatTargetHostIsotypeCloneApplications
ACTIN BETA antibodyDyLight®680HumanHuCALHuCAL Fab bivalent12141F, WB
ACTIN BETA antibodyDyLight®750HumanHuCALHuCAL Fab bivalent12141WB
ACTIN BETA antibodyDyLight®800HumanHuCALHuCAL Fab bivalent12141WB
ACTIN BETA antibodyHRPHumanHuCALHuCAL Fab bivalent12141WB
ACTIN BETA antibodyPurifiedHumanMouseIgG14C2C, IF, WB
ACTIN BETA antibodyPurifiedHumanHuCALHuCAL Fab bivalent12141WB
CYCLOPHILIN A antibodyBiotinHumanHuCALHuCAL Fab bivalent794E, WB
CYCLOPHILIN A antibodyDyLight®680HumanHuCALHuCAL Fab bivalent794WB
CYCLOPHILIN A antibodyDyLight®750HumanHuCALHuCAL Fab bivalent794WB
CYCLOPHILIN A antibodyDyLight®800HumanHuCALHuCAL Fab bivalent794WB
CYCLOPHILIN A antibodyHRPHumanHuCALHuCAL Fab bivalent794E, WB
CYCLOPHILIN A antibodyPreservative FreeHumanMouseIgG2a1F4-1B5WB
CYCLOPHILIN A antibodyPurifiedHumanHuCALHuCAL Fab bivalent794E, WB
GAPDH antibodyPurifiedHumanGoatPolyclonal IgGE, P *, WB
GAPDH antibodyBiotinRabbitMouseIgG16C5C, E, IF, IP, WB
GAPDH antibodyDyLight®680RabbitMouseIgG16C5WB
GAPDH antibodyDyLight®750RabbitMouseIgG16C5WB
GAPDH antibodyDyLight®800RabbitMouseIgG16C5WB
GAPDH antibodyHRPRabbitMouseIgG16C5C, E, WB
GAPDH antibodyPurifiedRabbitMouseIgG16C5C, E, IF, IP, WB
GAPDH antibodyPurifiedHumanRabbitPolyclonal IgGWB
PCNA antibodyFITCHumanMouseIgG2aPC10F *
PCNA antibodyPurifiedHumanMouseIgG2aPC10C, F *, IP, P, WB
PCNA antibodyPurifiedHumanRabbitPolyclonal IgGE, P *, WB
PCNA antibodyReady To UseHumanMouseIgG2aPC10C, P
TUBULIN ALPHA antibodyDyLight®680YeastRatIgG2aYL1/2WB
TUBULIN ALPHA antibodyDyLight®750YeastRatIgG2aYL1/2WB
TUBULIN ALPHA antibodyDyLight®800YeastRatIgG2aYL1/2WB
TUBULIN ALPHA antibodyHRPYeastRatIgG2aYL1/2C, E, WB
TUBULIN ALPHA antibodyPurifiedYeastRatIgG2aYL1/2C, E, IF, IP, R, WB
TUBULIN BETA antibodyPurifiedPigMouseIgMTU-13C, E, WB
VDAC1 antibodyPurifiedHumanRabbitPolyclonal IgGE, P *, WB

Western Blotting Secondary Antibodies

  • Ordinal Number: 119001
FormatTargetHostIsotypeCloneApplications
Ig antibody: Alk. Phos.HumanGoatPolyclonal IgGC, E, IB
IgA antibody: Alk. Phos.MouseGoatPolyclonal IgGE
IgA antibody: Alk. Phos.PigGoatPolyclonal IgGC, E, WB
IgA antibody: Alk. Phos.HumanGoatPolyclonal IgGE
IgA antibody: Alk. Phos.DogGoatPolyclonal IgGE
IgA antibody: Alk. Phos.GoatRabbitPolyclonal IgGE
IgA antibody: Alk. Phos.HorseGoatPolyclonal IgGE
IgA antibody: Alk. Phos.RatGoatPolyclonal IgGE, WB
IgA antibody: Alk. Phos.SheepRabbitPolyclonal IgGE
IgA antibody: Alk. Phos.ChickenGoatPolyclonal IgGE
IgA antibody: Alk. Phos.HumanGoatPolyclonal IgGC, E, WB
IgA antibody: Alk. Phos.RabbitGoatPolyclonal IgGE
IgA1 antibody: Alk. Phos.HumanMouseIgG1B3506B4C, E, IB, WB
IgE antibody: Alk. Phos.MouseGoatPolyclonal IgGE
IgE antibody: Alk. Phos.HumanGoatPolyclonal IgGE, WB
IgG antibody: Alk. Phos.BovineSheepPolyclonal IgGE, WB
IgG antibody: Alk. Phos.MouseRabbitPolyclonal IgGC, E, P, WB
IgG antibody: Alk. Phos.RatGoatPolyclonal IgGC, E, IB, WB
IgG antibody: Alk. Phos.PigGoatPolyclonal IgGE
IgG antibody: Alk. Phos.HumanGoatPolyclonal IgGE, WB
IgG antibody: Alk. Phos.PigGoatPolyclonal IgGE
IgG antibody: Alk. Phos.MouseGoatPolyclonal IgGC, E, WB
IgG antibody: Alk. Phos.HorseGoatPolyclonal IgGE
IgG antibody: Alk. Phos.RabbitDonkeyPolyclonal IgGC, E
IgG antibody: Alk. Phos.MonkeyGoatPolyclonal IgGE
IgG antibody: Alk. Phos.HumanGoatPolyclonal IgGC, E, WB
IgG antibody: Alk. Phos.ChickenRabbitPolyclonal IgGC, E, WB
IgG antibody: Alk. Phos.RatDonkeyPolyclonal IgGC, E, WB
IgG antibody: Alk. Phos.MouseGoatPolyclonal IgGE, WB
IgG IgA IgM antibody: Alk. Phos.MouseGoatPolyclonal IgGE
IgG2 antibody: Alk. Phos.DogSheepPolyclonal IgGE
IgG2 antibody: Alk. Phos.BovineSheepPolyclonal IgGE
IgG2b antibody: Alk. Phos.RatGoatPolyclonal IgGE, WB
IgG2b antibody: Alk. Phos.MouseGoatPolyclonal IgGC, E, P, WB
IgG2c antibody: Alk. Phos.RatGoatPolyclonal IgGE, WB
IgM antibody: Alk. Phos.ChickenGoatPolyclonal IgGE
IgM antibody: Alk. Phos.RabbitGoatPolyclonal IgGE
IgM antibody: Alk. Phos.RatGoatPolyclonal IgGE, WB
IgM antibody: Alk. Phos.SheepRabbitPolyclonal IgGE
IgM antibody: Alk. Phos.MouseGoatPolyclonal IgGC, E, P, WB
IgM antibody: Alk. Phos.DogGoatPolyclonal IgGE
KAPPA LIGHT CHAIN antibody: Alk. Phos.MouseGoatPolyclonal IgGC, E, IB
IgM antibody: AscitesMouseMOPC-104EWB
IgG antibody: BGALMouseGoatPolyclonal IgGE
IgG IgA IgM antibody: BGALMouseGoatPolyclonal IgGE
Ig antibody: BiotinRatGoatPolyclonal IgGE, IB, IF
IgA antibody: BiotinPigGoatPolyclonal IgGE, WB
IgA antibody: BiotinMouseGoatPolyclonal IgGE, IB, IF
IgA antibody: BiotinBovineSheepPolyclonal IgGE, WB
IgA antibody: BiotinHumanGoatPolyclonal IgGE
IgA1 antibody: BiotinHumanMouseIgG1B3506B4E, IB, WB
IgA2 antibody: BiotinHumanMouseIgG1A9604D2E
IgD antibody: BiotinRatMouseIgG1MARD-3E
IgE antibody: BiotinHumanGoatPolyclonal IgGE, WB
IgE antibody: BiotinMouseRatIgG1LO-ME-3E
IgE antibody: BiotinRatMouseIgG1MARE-1E, WB
IgG antibody: BiotinHamsterGoatPolyclonal IgGE, F
IgG antibody: BiotinMouseRabbitPolyclonal IgGC, E, F, P
IgG antibody: BiotinRatGoatPolyclonal IgGE, IB, IF
IgG antibody: BiotinGoatDonkeyPolyclonal IgGC, E
IgG antibody: BiotinRabbitSheepPolyclonal IgGC, E, P, WB
IgG antibody: BiotinPigGoatPolyclonal IgGE, WB
IgG antibody: BiotinRatGoatPolyclonal IgGC, E, IF, P, WB
IgG antibody: BiotinSheepRabbitPolyclonal IgGC, E, F, WB
IgG antibody: BiotinRatGoatPolyclonal IgGC, E, WB
IgG IgA IgM antibody: BiotinMouseGoatPolyclonal IgGE, IB
IgG IgM antibody: BiotinRabbitGoatPolyclonal IgGE
IgG1 antibody: BiotinBovineSheepPolyclonal IgGE, WB
IgG1 antibody: BiotinMouseGoatPolyclonal IgGE
IgG2a antibody: BiotinRatMouseIgG1MARG2a-1E
IgG2a antibody: BiotinMouseRatIgG1LO-MG2a-9E
IgG2b antibody: BiotinRatMouseIgG1MARG2b-8E
IgG2b antibody: BiotinMouseRatIgG1LO-MG2b-2E
IgM antibody: BiotinMouseRatIgG2aLO-MM-9E
IgM antibody: BiotinHumanGoatPolyclonal IgGE, F, IB
IgM antibody: BiotinMouseGoatPolyclonal IgGE, F, IB, IF
IgM antibody: BiotinRatMouseIgG1MARM-4E
IgM antibody: BiotinPigGoatPolyclonal IgGE, WB
KAPPA LIGHT CHAIN antibody: BiotinMouseGoatPolyclonal IgGE
KAPPA LIGHT CHAIN antibody: BiotinHumanGoatPolyclonal IgGE, IB, IF
KAPPA LIGHT CHAIN antibody: BiotinHumanGoatPolyclonal IgGE, IF, WB
KAPPA LIGHT CHAIN antibody: BiotinMouseRatIgG2bOX-20E, F
LAMBDA LIGHT CHAIN antibody: BiotinMouseGoatPolyclonal IgGE
IgG antibody: ConcentrateHumanRabbitPolyclonal IgGC, P *
IgG antibody: DyLight®405HamsterGoatPolyclonal IgGF, IF
IgG antibody: DyLight®488HamsterGoatPolyclonal IgGF, IF
IgG antibody: DyLight®488RatRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®488RabbitSheepPolyclonal IgGF, IF
IgG antibody: DyLight®488MouseGoatPolyclonal IgGF, IF
IgG antibody: DyLight®549RatRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®549MouseRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®549RatGoatPolyclonal IgGF, IF
IgG antibody: DyLight®549RabbitSheepPolyclonal IgGF, IF
IgG antibody: DyLight®549MouseGoatPolyclonal IgGF, IF
IgG antibody: DyLight®649HamsterGoatPolyclonal IgGF, IF
IgG antibody: DyLight®649MouseGoatPolyclonal IgGF, IF
IgG antibody: DyLight®800RatGoatPolyclonal IgGF, IF, WB
IgG antibody: DyLight®800MouseGoatPolyclonal IgGF, IF, WB
IgG antibody: DyLight®800SheepDonkeyPolyclonal IgGF, IF, WB
IgG antibody: DyLight®800MouseGoatPolyclonal IgGF, IF, WB
Ig antibody: FITCRatGoatPolyclonal IgGF
IgA antibody: FITCPigGoatPolyclonal IgGC, F
IgA antibody: FITCMouseGoatPolyclonal IgGC, F, IF
IgA antibody: FITCHumanMouseIgG115D6E, IF
IgA antibody: FITCDogGoatPolyclonal IgGC, F
IgA antibody: FITCSheepRabbitPolyclonal IgGF
IgA antibody: FITCHumanGoatPolyclonal IgGF, IF, P
IgA antibody: FITCHumanGoatPolyclonal IgGC, F, IF
IgD antibody: FITCHumanGoatPolyclonal IgGC, F, IF
IgE antibody: FITCRatMouseIgG1MARE-1F
IgE antibody: FITCHumanGoatPolyclonal IgGF
IgG antibody: FITCMouseRabbitPolyclonal IgGF
IgG antibody: FITCHumanGoatPolyclonal IgGC, F
IgG antibody: FITCCatGoatPolyclonal IgGC, F
IgG antibody: FITCHumanGoatPolyclonal IgGC, F
IgG antibody: FITCBovineSheepPolyclonal IgGC, F
IgG antibody: FITCGoatDonkeyPolyclonal IgGIF
IgG antibody: FITCHumanGoatPolyclonal IgGC, F, IF
IgG antibody: FITCMouseGoatPolyclonal IgGF
IgG antibody: FITCDogSheepPolyclonal IgGC, F, IF
IgG antibody: FITCHamsterGoatPolyclonal IgGF
IgG antibody: FITCPigGoatPolyclonal IgGC, F
IgG antibody: FITCHumanMouseIgG1MK 1 A6F
IgG antibody: FITCRabbitPolyclonal IgGF
IgG antibody: FITCRatGoatPolyclonal IgGC, F
IgG antibody: FITCSheepDonkeyPolyclonal IgGC
IgG antibody: FITCMouseGoatPolyclonal IgGF, IF
IgG antibody: FITCRabbitGoatPolyclonal IgGF
IgG antibody: FITCRabbitGoatPolyclonal IgGF, IF
IgG antibody: FITCRatGoatPolyclonal IgGF, IF
IgG antibody: FITCHorseGoatPolyclonal IgGC, F
IgG antibody: FITCGuinea PigGoatPolyclonal IgGC, F
IgG antibody: FITCMouseGoatPolyclonal IgGF
IgG antibody: FITCMouseGoatPolyclonal IgGF, IF
IgG antibody: FITCRabbitDonkeyPolyclonal IgGF
IgG IgA IgM antibody: FITCMouseGoatPolyclonal IgGF
IgG IgM antibody: FITCRabbitGoatPolyclonal IgGF
IgG1 antibody: FITCRatMouseIgG1MARG1-2F
IgG1 antibody: FITCMouseRatIgG1LO-MG1-2F
IgG1 antibody: FITCRatGoatPolyclonal IgGF
IgG1 antibody: FITCDogGoatPolyclonal IgGF
IgG2 antibody: FITCBovineSheepPolyclonal IgGC, F
IgG2 antibody: FITCDogSheepPolyclonal IgGF
IgG2a antibody: FITCRatGoatPolyclonal IgGF
IgG2a antibody: FITCMouseHuCALHuCAL Fab bivalent6344F
IgG2a antibody: FITCMouseRatIgG1LO-MG2a-7F
IgG2a antibody: FITCMouseRabbitPolyclonal IgGIF
IgG2a antibody: FITCMouseRatIgG1LO-MG2a-9F
IgG2a antibody: FITCMouseGoatPolyclonal IgGC, F
IgG2b antibody: FITCMouseRatIgG1LO-MG2b-2F
IgG2b antibody: FITCRatMouseIgG1MARG2b-3F
IgG2b antibody: FITCMouseHuCALHuCAL Fab bivalent4642F
IgG2c antibody: FITCRatGoatPolyclonal IgGF
IgG2c antibody: FITCMouseGoatPolyclonal IgGF
IgG3 antibody: FITCMouseGoatPolyclonal IgGC, F, IF
IgG3 antibody: FITCMouseHuCALHuCAL Fab bivalent4582F
IgG3 antibody: FITCHumanSheepPolyclonal IgGIF
IgM antibody: FITCSheepRabbitPolyclonal IgGF
IgM antibody: FITCSheepMouseIgG125.69F
IgM antibody: FITCChickenGoatPolyclonal IgGC, F
IgM antibody: FITCHumanMouseIgG1M15/8F
IgM antibody: FITCMouseHuCALHuCAL Fab bivalent4620F
IgM antibody: FITCCatGoatPolyclonal IgGC, F
IgM antibody: FITCRabbitGoatPolyclonal IgGF
IgM antibody: FITCMouseGoatPolyclonal IgGF
IgM antibody: FITCDogGoatPolyclonal IgGC, F
IgM antibody: FITCRatGoatPolyclonal IgGIF
IgM antibody: FITCMouseGoatPolyclonal IgGIF
KAPPA LIGHT CHAIN antibody: FITCHumanSheepPolyclonal IgGC, E, IF
KAPPA LIGHT CHAIN antibody: FITCMouseGoatPolyclonal IgGF
KAPPA LIGHT CHAIN antibody: FITCMouseRatIgG2bOX-20F
KAPPA LIGHT CHAIN antibody: FITCHumanGoatPolyclonal IgGC, F, IF
KAPPA LIGHT CHAIN antibody: FITCRatMouseIgG2aOX-12F
LAMBDA LIGHT CHAIN antibody: FITCHumanGoatPolyclonal IgGC, F
LAMBDA LIGHT CHAIN antibody: FITCHumanGoatPolyclonal IgGC, F, IF
Ig antibody: HRPHumanGoatPolyclonal IgGE
Ig antibody: HRPRatGoatPolyclonal IgGC, E, IB
IgA antibody: HRPGoatRabbitPolyclonal IgGE
IgA antibody: HRPHumanGoatPolyclonal IgGE, P, WB
IgA antibody: HRPDogGoatPolyclonal IgGC, E
IgA antibody: HRPRatGoatPolyclonal IgGE, WB
IgA antibody: HRPChickenGoatPolyclonal IgGE
IgA antibody: HRPBovineSheepPolyclonal IgGE
IgA antibody: HRPHorseGoatPolyclonal IgGE
IgA antibody: HRPMouseGoatPolyclonal IgGC, E, P, WB
IgA antibody: HRPPigGoatPolyclonal IgGE
IgA antibody: HRPHumanGoatPolyclonal IgGC, E, WB
IgA1 antibody: HRPHumanMouseIgG1B3506B4E
IgA2 antibody: HRPHumanMouseIgG1A9604D2E, IB, WB
IgE antibody: HRPMouseRatIgG1LO-ME-3E
IgE antibody: HRPDogGoatPolyclonal IgGC, E, WB
IgE antibody: HRPRatMouseIgG1MARE-1E, WB
IgE antibody: HRPHumanMouseIgG11A2E
IgE antibody: HRPMouseHuCALHuCAL Fab bivalent4658E
IgE antibody: HRPRatSheepPolyclonal IgGC, E, WB
IgG antibody: HRPMouseGoatPolyclonal IgGC, E, P
IgG antibody: HRPRabbitGoatPolyclonal IgGE
IgG antibody: HRPHumanGoatPolyclonal IgGE, WB
IgG antibody: HRPRabbitSheepPolyclonal IgGC, E, WB
IgG antibody: HRPHumanGoatPolyclonal IgGC, E, WB
IgG antibody: HRPPigGoatPolyclonal IgGE
IgG antibody: HRPBovineSheepPolyclonal IgGE, WB
IgG antibody: HRPMouseRabbitPolyclonal IgGC, E, P, RE, WB
IgG antibody: HRPGoatDonkeyPolyclonal IgGE, WB
IgG antibody: HRPRatGoatPolyclonal IgGC, E, IB
IgG antibody: HRPHumanMouseIgG1MK 1 A6C *, E
IgG antibody: HRPRabbitGoatPolyclonal IgGC, E, WB
IgG antibody: HRPDogSheepPolyclonal IgGE
IgG antibody: HRPHumanHuCALHuCAL Fab monovalent5985E
IgG antibody: HRPGoatDonkeyPolyclonal IgGC, E
IgG antibody: HRPRatRabbitPolyclonal IgGC, E, P, RE
IgG antibody: HRPRatRabbitPolyclonal IgGC, E, IB
IgG antibody: HRPRabbitGoatPolyclonal IgGWB
IgG antibody: HRPCatGoatPolyclonal IgGE
IgG antibody: HRPMouseGoatPolyclonal IgGE
IgG antibody: HRPMouseGoatPolyclonal IgGE, WB
IgG antibody: HRPRatGoatPolyclonal IgGC, E, WB
IgG antibody: HRPHorseGoatPolyclonal IgGE, WB
IgG antibody: HRPRabbitGoatPolyclonal IgGC, E, WB
IgG antibody: HRPMouseGoatPolyclonal IgGC, E, WB
IgG antibody: HRPRatDonkeyPolyclonal IgGC, E
IgG antibody: HRPSheepRabbitPolyclonal IgGC, E, WB
IgG antibody: HRPMouseSheepPolyclonal IgGC *, E, WB
IgG antibody: HRPMonkeyGoatPolyclonal IgGE
IgG antibody: HRPRabbitGoatPolyclonal IgGE, WB
IgG antibody: HRPRabbitDonkeyPolyclonal IgGC, E
IgG antibody: HRPRabbitGoatPolyclonal IgGC, E, WB
IgG IgA IgM antibody: HRPHumanGoatPolyclonal IgGE, WB
IgG IgA IgM antibody: HRPMouseGoatPolyclonal IgGE, WB
IgG IgM antibody: HRPRabbitGoatPolyclonal IgGE
IgG1 antibody: HRPRatGoatPolyclonal IgGE, WB
IgG1 antibody: HRPBovineMouseIgG1IL-A60E
IgG1 antibody: HRPMouseHuCALHuCAL Fab bivalent4639E
IgG1 antibody: HRPRatMouseIgG1MARG1-2E
IgG1 antibody: HRPMouseGoatPolyclonal IgGC, E, P, WB
IgG1 antibody: HRPRatHuCALHuCAL Fab bivalent8941E
IgG1 antibody: HRPMouseRatIgG1LO-MG1-2E
IgG1 antibody: HRPMouseRabbitPolyclonal IgGC, E, IB, WB
IgG1 antibody: HRPRatHuCALHuCAL Fab bivalent8938E
IgG2a antibody: HRPMouseGoatPolyclonal IgGC, E, P, WB
IgG2a antibody: HRPRatHuCALHuCAL Fab bivalent9345E
IgG2a antibody: HRPMouseRatIgG1LO-MG2a-7E
IgG2b antibody: HRPRatGoatPolyclonal IgGC, E, P, WB
IgG2b antibody: HRPMouseGoatPolyclonal IgGC, E, P, WB
IgG2b antibody: HRPMouseHuCALHuCAL Fab bivalent4642E
IgG2c antibody: HRPRatGoatPolyclonal IgGE, WB
IgG3 antibody: HRPHumanMouseIgG1HP6050C, E, WB
IgG3 antibody: HRPMouseHuCALHuCAL Fab bivalent4582E
IgG4 antibody: HRPHumanMouseIgG1HP6025C, E, P
IgGb antibody: HRPHorseGoatPolyclonal IgGE, WB
IgGc antibody: HRPHorseGoatPolyclonal IgGE, WB
IgM antibody: HRPHorseGoatPolyclonal IgGE
IgM antibody: HRPMouseHuCALHuCAL Fab bivalent4620E
IgM antibody: HRPRatGoatPolyclonal IgGE, WB
IgM antibody: HRPRatMouseIgG1MARM-4E
IgM antibody: HRPMouseGoatPolyclonal IgGC, E, P
IgM antibody: HRPDogGoatPolyclonal IgGE
IgM antibody: HRPHumanGoatPolyclonal IgGC, E, P, WB
IgM antibody: HRPRatGoatPolyclonal IgGC, E, IB
IgM antibody: HRPCatGoatPolyclonal IgGE
IgM antibody: HRPBovineMouseIgG1IL-A30E
KAPPA LIGHT CHAIN antibody: HRPMouseGoatPolyclonal IgGC, E, IB
KAPPA LIGHT CHAIN antibody: HRPMouseRatIgG2aLO-MK-1C, E
KAPPA LIGHT CHAIN antibody: HRPHumanGoatPolyclonal IgGC, E, P, WB
KAPPA LIGHT CHAIN antibody: HRPRatMouseIgG2aOX-12E
KAPPA/LAMBDA LIGHT CHAIN antibody: HRPRatMouseIgG1MARK-1/MARL-15E
LAMBDA LIGHT CHAIN antibody: HRPMouseGoatPolyclonal IgGE
LAMBDA LIGHT CHAIN antibody: HRPHumanGoatPolyclonal IgGC, E, P, WB
IgE antibody: KitMouseE
ALPHA 1 MICROGLOBULIN antibody: PurifiedHumanE
BETA 2 MICROGLOBULIN antibody: PurifiedHumanE, WB
C1q antibody: PurifiedHumanE
C4c antibody: PurifiedHumanE
Ig antibody: PurifiedHumanGoatPolyclonal IgGE, IB
IgA antibody: PurifiedRatMouseIgG1MARA-2E, R
IgA antibody: PurifiedMonkeyMouseIgG1IgA5-3BE, WB
IgA antibody: PurifiedHumanMouseIgG1Mc29-12C, E, P
IgA antibody: PurifiedRatMouseIgG1MARA-1C, E, IF
IgA antibody: PurifiedPigGoatPolyclonal IgGE, ID
IgA antibody: PurifiedMouseRatIgMLO-MA-10E
IgA antibody: PurifiedMouseGoatPolyclonal IgGC, E
IgA antibody: PurifiedBovineMouseIgG2aIL-A71E, IP
IgA antibody: PurifiedSheepRabbitPolyclonal IgGE
IgA antibody: PurifiedHumanGoatPolyclonal IgGC, E, WB
IgA antibody: PurifiedHumanMouseIgG115D6E
IgA antibody: PurifiedHumanMouseIgG1Mc24-2E11C, E, IP, WB
IgA antibody: PurifiedRatGoatPolyclonal IgGE, IF, WB
IgA antibody: PurifiedRabbitGoatPolyclonal IgGE, ID
IgA1 antibody: PurifiedHumanMouseIgG1B3506B4C, E, IB, WB
IgA2 antibody: PurifiedHumanMouseIgG1A9604D2C, E, IB, WB
IgD antibody: PurifiedMouseRatIgG2aLO-MD-6E
IgD antibody: PurifiedRatMouseIgG1MARD-3C, E
IgD antibody: PurifiedHumanGoatPolyclonal IgGE
IgD antibody: PurifiedHumanMouseIgG2aIADB6E
IgD antibody: PurifiedHumanMouseIgG1LE2C
IgE antibody: PurifiedMouseGoatPolyclonal IgGE
IgE antibody: PurifiedMouseGoatPolyclonal IgGE, WB
IgE antibody: PurifiedDogMouseIgG2aE6-2A1E, WB
IgE antibody: PurifiedHumanE
IgE antibody: PurifiedMouseRatIgG1LO-ME-3E
IgE antibody: PurifiedMouseHuCALHuCAL Fab bivalent4658E
IgE antibody: PurifiedHumanMouseIgG2aE454 (5D5)E
IgE antibody: PurifiedRatIgEE
IgE antibody: PurifiedMouseHuCALHuCAL Fab bivalent4677E
IgE antibody: PurifiedHumanGoatPolyclonal IgGE, WB
IgE antibody: PurifiedDogMouseIgG1E6-71A1E, WB
IgE antibody: PurifiedHumanMouseIgG2aE411 (5H2)E, F
IgE antibody: PurifiedMouseSheepPolyclonal IgGID
IgG antibody: PurifiedHumanGoatPolyclonal IgGE, IF, WB
IgG antibody: PurifiedHumanGoatPolyclonal IgGE
IgG antibody: PurifiedHumanGoatPolyclonal IgGE
IgG antibody: PurifiedRabbitPolyclonal IgGE
IgG antibody: PurifiedBovineSheepPolyclonal IgGE, ID
IgG antibody: PurifiedBovineGoatPolyclonal IgGE, IF, WB
IgG antibody: PurifiedGoatE
IgG antibody: PurifiedHamsterGoatPolyclonal IgGE
IgG antibody: PurifiedHumanHuCALHuCAL Fab bivalent6860E
IgG antibody: PurifiedHumanGoatPolyclonal IgGC, E
IgG antibody: PurifiedCatGoatPolyclonal IgGE, ID
IgG antibody: PurifiedRatE
IgG antibody: PurifiedMouseGoatPolyclonal IgGE
IgG antibody: PurifiedMouseGoatPolyclonal IgGE
IgG antibody: PurifiedHumanMouseIgG1R10Z8E9E
IgG antibody: PurifiedSheepE
IgG antibody: PurifiedHumanHuCALHuCAL Fab monovalent5985E
IgG antibody: PurifiedMouseRabbitPolyclonal IgGC, E, P
IgG antibody: PurifiedGoatRabbitPolyclonal IgGE, IF, WB
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE, WB
IgG antibody: PurifiedDogIgGN/AE
IgG antibody: PurifiedCatMouseIgG1GPB2-2B1E, WB
IgG antibody: PurifiedRabbitSheepPolyclonal IgGC, E, WB
IgG antibody: PurifiedMouseIY
IgG antibody: PurifiedHumanGoatPolyclonal IgGE, IF, WB
IgG antibody: PurifiedGoatRabbitPolyclonal IgGE
IgG antibody: PurifiedRhesus MonkeyHuCALHuCAL Fab bivalent8717E
IgG antibody: PurifiedHumanMouseIgG1B33/20E
IgG antibody: PurifiedHumanHuCALHuCAL Fab bivalent6863E
IgG antibody: PurifiedPigGoatPolyclonal IgGE, ID, WB
IgG antibody: PurifiedPigE
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE, IB
IgG antibody: PurifiedHumanGoatPolyclonal IgGE
IgG antibody: PurifiedGuinea PigRabbitPolyclonal IgGE
IgG antibody: PurifiedGuinea PigGoatPolyclonal IgGE
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE, WB
IgG antibody: PurifiedHumanGoatPolyclonal IgGE, IP, WB
IgG antibody: PurifiedMouseGoatPolyclonal IgGE
IgG antibody: PurifiedRabbitGoatPolyclonal IgGC, E, WB
IgG antibody: PurifiedHumanMouseIgMA57HC, E, H, P, WB
IgG antibody: PurifiedRatRabbitPolyclonal IgGE
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE, IB
IgG antibody: PurifiedSheepRabbitPolyclonal IgGC, E, WB
IgG antibody: PurifiedMonkeyGoatPolyclonal IgGE, ID
IgG antibody: PurifiedChickenRabbitPolyclonal IgGC, E, WB
IgG antibody: PurifiedHorseGoatPolyclonal IgGE, ID
IgG antibody: PurifiedMouseGoatPolyclonal IgGC, E, WB
IgG antibody: PurifiedMouseRabbitPolyclonal IgGC, E, IF, WB
IgG antibody: PurifiedGoatRabbitPolyclonal IgGE
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE
IgG antibody: PurifiedHorseGoatPolyclonal IgGE
IgG antibody: PurifiedMouseGoatPolyclonal IgGE, WB
IgG IgA IgM antibody: PurifiedHumanGoatPolyclonal IgGE
IgG IgM antibody: PurifiedRabbitGoatPolyclonal IgGE, IB
IgG1 antibody: PurifiedMouseIgG1E
IgG1 antibody: PurifiedMouseIgG1MADNP-1E, GS
IgG1 antibody: PurifiedHumanE, FN*
IgG1 antibody: PurifiedMouseE
IgG1 antibody: PurifiedPigMouseIgG1K139 3C8E
IgG1 antibody: PurifiedMouseGoatPolyclonal IgGE
IgG1 antibody: PurifiedMouseGoatPolyclonal IgGE, IF, WB
IgG1 antibody: PurifiedHumanMouseIgG12C11E
IgG1 antibody: PurifiedRatHuCALHuCAL Fab bivalent8938E
IgG1 antibody: PurifiedMouseHuCALHuCAL Fab bivalent4639E
IgG1 antibody: PurifiedRatHuCALHuCAL Fab bivalent8941E
IgG1 antibody: PurifiedBovineE
IgG1 LAMBDA MYELOMA antibody: PurifiedRatIR31E
IgG2 antibody: PurifiedBovineE
IgG2 antibody: PurifiedBovineMouseIgG1IL-A73E, F, IP
IgG2 antibody: PurifiedBovineSheepPolyclonal IgGE, ID
IgG2 antibody: PurifiedDogSheepPolyclonal IgGC, E, WB
IgG2 antibody: PurifiedGuinea PigGoatPolyclonal IgGE
IgG2 antibody: PurifiedHumanMouseIgG13C7E
IgG2 antibody: PurifiedHumanE
IgG2 antibody: PurifiedHumanMouseIgG1HP6014C, E
IgG2a antibody: PurifiedRatMouseIgG1MARG2a-1E
IgG2a antibody: PurifiedRatHuCALHuCAL Fab bivalent9345E
IgG2a antibody: PurifiedMouseHuCALHuCAL Fab bivalent6344E, F
IgG2a antibody: PurifiedMouseRatIgG1LO-MG2a-9E
IgG2a antibody: PurifiedMouseGoatPolyclonal IgGE
IgG2b antibody: PurifiedRatMouseIgG1MARG2b-3E
IgG2b antibody: PurifiedMouseIgG2bMADNP-3E
IgG2b antibody: PurifiedRatIR863E
IgG2b antibody: PurifiedMouseGoatPolyclonal IgGE
IgG2b antibody: PurifiedRatHuCALHuCAL Fab bivalent9204E
IgG2c antibody: PurifiedMouseGoatPolyclonal IgGE
IgG2c antibody: PurifiedRatHuCALHuCAL Fab bivalent9337E, F
IgG2c antibody: PurifiedRatGoatPolyclonal IgGE, F, WB
IgG3 antibody: PurifiedHumanMouseIgG15G12E
IgG3 antibody: PurifiedMouseRatIgMLO-MG3-7E
IgG3 antibody: PurifiedMouseIgG3MADNP-4E
IgG4 antibody: PurifiedHumanE
IgG4 antibody: PurifiedHumanMouseIgG1HP6025C, IB, P, WB
IgG4 antibody: PurifiedHumanMouseIgG3HP6023C, E
IgGa antibody: PurifiedHorseMouseIgG1CVS48E, F
IgGb antibody: PurifiedHorseMouseIgG1CVS39E
IgM antibody: PurifiedBovineSheepPolyclonal IgGE, ID
IgM antibody: PurifiedMouseHuCALHuCAL Fab bivalent10922E
IgM antibody: PurifiedMouseGoatPolyclonal IgGE, IF, WB
IgM antibody: PurifiedBovineMouseIgG1IL-A30E, F, IP, R
IgM antibody: PurifiedHumanRatIgMHM-7E
IgM antibody: PurifiedRatGoatPolyclonal IgGC, E, WB
IgM antibody: PurifiedMouseHuCALHuCAL Fab bivalent4620E, F
IgM antibody: PurifiedRabbitIgMN/AE
IgM antibody: PurifiedHumanGoatPolyclonal IgGE
IgM antibody: PurifiedRatGoatPolyclonal IgGE, F, WB
IgM antibody: PurifiedHumanMouseIgG147.2.E9E
IgM antibody: PurifiedHumanIgME
IgM antibody: PurifiedHumanGoatPolyclonal IgGE
IgM antibody: PurifiedMouseGoatPolyclonal IgGC, E, WB
IgM antibody: PurifiedHorseGoatPolyclonal IgGE, ID
IgM antibody: PurifiedMouseGoatPolyclonal IgGE, F, IF
IgM antibody: PurifiedMouseHuCALHuCAL Fab bivalent10921E
IgM antibody: PurifiedRabbitGoatPolyclonal IgGE, IB
IgM antibody: PurifiedSheepRabbitPolyclonal IgGE
IgM antibody: PurifiedHumanE
IgM antibody: PurifiedHumanMouseIgG1M15/8C, E, F, IP
IgM antibody: PurifiedRatMouseIgG1MARM-4E
IgM antibody: PurifiedRatIR473E
IgM antibody: PurifiedPigGoatPolyclonal IgGE, ID
IgM antibody: PurifiedGoatRabbitPolyclonal IgGE, ID
J CHAIN antibody: PurifiedHumanMouseIgG1Mc19-9C, E, IP, WB
KAPPA LIGHT CHAIN antibody: PurifiedHumanGoatPolyclonal IgGE
KAPPA LIGHT CHAIN antibody: PurifiedHumanMouseIgG2a4G7E
KAPPA LIGHT CHAIN antibody: PurifiedHumanSheepPolyclonal IgGC, E
KAPPA LIGHT CHAIN antibody: PurifiedMouseRatIgG2bOX-20F
KAPPA LIGHT CHAIN antibody: PurifiedMouseGoatPolyclonal IgGE, WB
KAPPA LIGHT CHAIN antibody: PurifiedHumanGoatPolyclonal IgGE
KAPPA LIGHT CHAIN antibody: PurifiedHumanGoatPolyclonal IgGE, IF, WB
LAMBDA LIGHT CHAIN antibody: PurifiedRatMouseIgG1MARL-15C, E, WB
LAMBDA LIGHT CHAIN antibody: PurifiedHumanMouseIgG2a2G9E
LAMBDA LIGHT CHAIN antibody: PurifiedHumanGoatPolyclonal IgGE, IF, WB
LAMBDA LIGHT CHAIN antibody: PurifiedHumanGoatPolyclonal IgGE, IF, WB
LAMBDA LIGHT CHAIN antibody: PurifiedHumanMouseIgG1Mc24-IC6E, F
LAMBDA LIGHT CHAIN antibody: PurifiedMouseGoatPolyclonal IgGE, IB, IF, WB
PROTEIN S antibody: PurifiedHumanE
KAPPA LIGHT CHAIN antibody: Ready To UseHumanRabbitPolyclonal IgGC, P *
IgA antibody: RPEMouseGoatPolyclonal IgGF
IgA antibody: RPEHumanGoatPolyclonal IgGF
IgD antibody: RPEHumanGoatPolyclonal IgGE, F
IgG antibody: RPEMouseGoatPolyclonal IgGF
IgG antibody: RPERatGoatPolyclonal IgGF
IgG antibody: RPERatGoatPolyclonal IgGF
IgG antibody: RPEHamsterGoatPolyclonal IgGF
IgG antibody: RPERatGoatPolyclonal IgGF
IgG antibody: RPERatDonkeyPolyclonal IgGF
IgG antibody: RPEMouseGoatPolyclonal IgGF, IF
IgG antibody: RPEMouseGoatPolyclonal IgGF
IgG IgA IgM antibody: RPEMouseGoatPolyclonal IgGF
IgG1 antibody: RPEMouseGoatPolyclonal IgGF
IgG2a antibody: RPEMouseGoatPolyclonal IgGF
IgG2b antibody: RPEMouseHuCALHuCAL Fab bivalent4642F
IgG2b antibody: RPEMouseGoatPolyclonal IgGF
IgG3 antibody: RPEMouseHuCALHuCAL Fab bivalent4582F
IgM antibody: RPEHumanGoatPolyclonal IgGF
IgM antibody: RPERatGoatPolyclonal IgGF
KAPPA LIGHT CHAIN antibody: RPEHumanGoatPolyclonal IgGF
KAPPA LIGHT CHAIN antibody: RPEMouseGoatPolyclonal IgGF
LAMBDA LIGHT CHAIN antibody: RPEHumanMouseIgG1Mc24-IC6F
LAMBDA LIGHT CHAIN antibody: RPEHumanGoatPolyclonal IgGF
IgA antibody: S/NCatMouseIgG1K218 3E10E
IgA antibody: S/NHorseMouseIgG1K129.2G5E
IgA antibody: S/NBovineMouseIgG1K84 2F9E
IgG antibody: S/NCatMouseIgG1K62 2G4E
IgG IgA IgM antibody: S/NDogMouseIgG1K99 2E3E
IgG IgA IgM antibody: S/NCatMouseIgG1K185 4D4E
IgG2 antibody: S/NBovineMouseIgG1K192 4F10E
LAMBDA LIGHT CHAIN antibody: S/NPigMouseIgG2aK139 3E1C, E, F, P
IgG antibody: SerumGuinea PigSheepPolyclonal IgGE, ID
IgG antibody: SerumChickenSheepPolyclonal IgGIP
IgM antibody: SerumHumanSheepPolyclonal IgGE, ID, IP
LAMBDA LIGHT CHAIN antibody: SerumHumanGoatPolyclonal IgGTB
Ig antibody: Texas Red®RatGoatPolyclonal IgGF
IgG antibody: Texas Red®MouseGoatPolyclonal IgGIF
IgG IgA IgM antibody: Texas Red®MouseGoatPolyclonal IgGF
IgG IgM antibody: Texas Red®RabbitGoatPolyclonal IgGF
IgG1 antibody: Texas Red®MouseGoatPolyclonal IgGF
IgG2a antibody: Texas Red®MouseGoatPolyclonal IgGF
IgM antibody: Texas Red®MouseGoatPolyclonal IgGF
IgM antibody: Texas Red®HumanGoatPolyclonal IgGF
KAPPA LIGHT CHAIN antibody: Texas Red®MouseGoatPolyclonal IgGF
LAMBDA LIGHT CHAIN antibody: Texas Red®HumanGoatPolyclonal IgGF
Ig antibody: TRITCHumanGoatPolyclonal IgGC, F
IgG antibody: TRITCMouseGoatPolyclonal IgGIF
IgG IgM antibody: TRITCRabbitGoatPolyclonal IgGF
IgM antibody: TRITCRatGoatPolyclonal IgGIF
KAPPA LIGHT CHAIN antibody: TRITCMouseGoatPolyclonal IgGF
LAMBDA LIGHT CHAIN antibody: TRITCMouseGoatPolyclonal IgGF, IF

Horseradish-peroxidase conjugated antibodies are preferentially used whenever an indirect method is used for detection on Western Blots. AbD Serotec supplies a range of species specific antibodies recognizing immunoglobulin or any of its isotypes. Monoclonal and polyclonal secondary antibodies are available, as a whole IgG or a F(ab’)2 fragment. Performance is further enhanced by affinity purification for all STAR reagents and many anti-mouse, -rat, and -hamster secondary antibodies are multi-species adsorbed.


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