What is Western Blotting?

Western Blotting

Western blotting, also known as immunoblotting or protein blotting, is a core technique in cell and molecular biology . In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells . 

The Western blotting procedure relies upon three key elements to accomplish this task:

  • the separation of protein mixtures by size using gel electrophoresis;
  • the efficient transfer of separated proteins to a solid support;
  • and the specific detection of a target protein by appropriately matched antibodies.

Once detected, the target protein will be visualized as a band on a blotting membrane, X-ray film, or an imaging system . Since Western blotting is accomplished rapidly, using simple equipment and inexpensive reagents, it is one of the most common laboratory techniques . The results achieved are also easy to interpret, unique, and unambiguous . Therefore, it is routinely used on its own, or along with other immunoassays, in research and clinical settings.


Why use a Western Blot?

Western blots are in wide use across a broad range of scientific and clinical disciplines . Their ability to clearly show the presence of a specific protein both by size and through the binding of an antibody makes them well-suited for evaluating levels of protein expression in cells, and for monitoring fractions during protein purification . Likewise, they are helpful for comparing expression of a target protein from various tissues, or seeing how a particular protein responds to disease or drug treatment .

In many cases, Western blots are used in combination with other key antibody based detection techniques, such as ELISAs or immunohistochemistry.

Western Blotting Antibodies

Antibodies selected for immunodetection should be Western blot tested if possible, with attention paid to the experimental conditions recommended by the antibody supplier . Usually, Western blot positive antibodies recognize a short linear sequence of amino acids found within the target protein that remains intact, or becomes visible, when the target protein is fully unraveled . This is because most Western blots are carried out under denaturing and reducing conditions which remove all higher order protein structure .

In contrast, some epitopes can be conformational, forming a three dimensional structural configuration of amino acids that will be lost upon denaturation of the protein . Thus, not all antibodies work in a typical Western blot . Since Western blot procedures allow for flexibility in choosing gel electrophoresis and blotting conditions, it is possible to modify buffers to retain enough higher order protein structure for detection by some antibodies . 

AbD Serotec is one of the world’s leading manufacturers of primary and secondary antibodies for Western blot anaylsis. In addition, we offer ISO-certified pre-made buffers to simplify immunostaining and make results more consistent from blot to blot. Our 100% Satisfaction Guarantee is backed by our quality, which makes ordering from AbD Serotec totally risk free!


Western Blotting Buffers & Accessories

  • Ordinal Number: 9015
SpecificityFormatTargetHostIsotypeCloneApplications
AP STABILIZING DILUENT antibodyReady To UseC, E, P, WB
BLOCK ACE antibodyReagentE, WB
HORSERADISH PEROXIDASE antibodyReagentE, WB
HRP-STABILIZING DILUENT antibodyReady To UseC, E, P, WB
PROTEIN STABILIZER antibodyReady To UseIY

 

Our range of specialized reagents are designed to significantly improve results in Western blotting:

  • Block Ace This high-performance blocking reagent for Western blotting is superior to 1% BSA. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves. It can also be used as a wash buffer, and for diluting antibodies.
  • HiSpec This assay diluent works to significantly reduce cross-reactivity and non-specific binding in Western blotting, greatly improving your results. HiSpec assay diluent is designed to be used instead of sample buffer or antibody dilution buffer within the Western blotting protocol.
  • AbGuard® HRP Stabilizer Is ideal for use as a diluent for peroxidase conjugated antibodies and other peroxidase conjugated proteins. It provides superior stabilization of HRP-conjugates at low and high dilutions, preventing the loss of peroxidise activity.

Western Blotting Loading Control Antibodies

AbD Serotec offers a range of antibodies to popular loading controls suitable for Western blotting. Our loading control antibodies are offered in a variety of different formats ideal for saving time with direct staining techniques and for use in infrared imaging systems e.g. LI-COR Odyssey®.

Western Blotting Loading Control Antibodies

  • Ordinal Number: 9021
SpecificityFormatTargetHostIsotypeCloneApplications
ACTIN BETA antibodyDyLight®680HumanHuCALHuCAL Fab bivalent12141F, WB
ACTIN BETA antibodyDyLight®750HumanHuCALHuCAL Fab bivalent12141WB
ACTIN BETA antibodyDyLight®800HumanHuCALHuCAL Fab bivalent12141WB
ACTIN BETA antibodyHRPHumanHuCALHuCAL Fab bivalent12141WB
ACTIN BETA antibodyPurifiedHumanMouseIgG14C2C, IF, WB
ACTIN BETA antibodyPurifiedHumanHuCALHuCAL Fab bivalent12141WB
CYCLOPHILIN A antibodyBiotinHumanHuCALHuCAL Fab bivalent794E, WB
CYCLOPHILIN A antibodyDyLight®680HumanHuCALHuCAL Fab bivalent794WB
CYCLOPHILIN A antibodyDyLight®750HumanHuCALHuCAL Fab bivalent794WB
CYCLOPHILIN A antibodyDyLight®800HumanHuCALHuCAL Fab bivalent794WB
CYCLOPHILIN A antibodyHRPHumanHuCALHuCAL Fab bivalent794E, WB
CYCLOPHILIN A antibodyPreservative FreeHumanMouseIgG2a1F4-1B5WB
CYCLOPHILIN A antibodyPurifiedHumanHuCALHuCAL Fab bivalent794E, WB
GAPDH antibodyPurifiedHumanGoatPolyclonal IgGE, P *, WB
GAPDH antibodyBiotinRabbitMouseIgG16C5C, E, IF, IP, WB
GAPDH antibodyDyLight®680RabbitMouseIgG16C5WB
GAPDH antibodyDyLight®750RabbitMouseIgG16C5WB
GAPDH antibodyDyLight®800RabbitMouseIgG16C5WB
GAPDH antibodyHRPRabbitMouseIgG16C5C, E, WB
GAPDH antibodyPurifiedRabbitMouseIgG16C5C, E, IF, IP, WB
GAPDH antibodyPurifiedHumanRabbitPolyclonal IgGWB
PCNA antibodyFITCHumanMouseIgG2aPC10F *
PCNA antibodyPurifiedHumanMouseIgG2aPC10C, F *, IP, P, WB
PCNA antibodyPurifiedHumanRabbitPolyclonal IgGE, P *, WB
PCNA antibodyReady To UseHumanMouseIgG2aPC10C, P
TUBULIN ALPHA antibodyDyLight®680YeastRatIgG2aYL1/2WB
TUBULIN ALPHA antibodyDyLight®750YeastRatIgG2aYL1/2WB
TUBULIN ALPHA antibodyDyLight®800YeastRatIgG2aYL1/2WB
TUBULIN ALPHA antibodyHRPYeastRatIgG2aYL1/2C, E, WB
TUBULIN ALPHA antibodyPurifiedYeastRatIgG2aYL1/2C, E, IF, IP, R, WB
TUBULIN BETA antibodyPurifiedPigMouseIgMTU-13C, E, WB
VDAC1 antibodyPurifiedHumanRabbitPolyclonal IgGE, P *, WB

Western Blotting Secondary Antibodies

  • Ordinal Number: 119001
FormatTargetHostIsotypeCloneApplications
Ig antibody: Alk. Phos.HumanGoatPolyclonal IgGC, E, IB
Ig antibody: Alk. Phos.RatGoatPolyclonal IgGC, E, IB
IgA antibody: Alk. Phos.MouseGoatPolyclonal IgGE
IgA antibody: Alk. Phos.PigGoatPolyclonal IgGC, E, WB
IgA antibody: Alk. Phos.HumanGoatPolyclonal IgGE
IgA antibody: Alk. Phos.BovineSheepPolyclonal IgGC, E, WB
IgA1 antibody: Alk. Phos.HumanMouseIgG1B3506B4C, E, IB, WB
IgE antibody: Alk. Phos.MouseGoatPolyclonal IgGE
IgE antibody: Alk. Phos.HumanGoatPolyclonal IgGE, WB
IgG antibody: Alk. Phos.MouseRabbitPolyclonal IgGC, E, P, WB
IgG antibody: Alk. Phos.MouseGoatPolyclonal IgGE
IgG antibody: Alk. Phos.PigGoatPolyclonal IgGE
IgG antibody: Alk. Phos.HumanGoatPolyclonal IgGE, WB
IgG antibody: Alk. Phos.PigGoatPolyclonal IgGE
IgG antibody: Alk. Phos.ChickenGoatPolyclonal IgGE
IgG antibody: Alk. Phos.HorseGoatPolyclonal IgGE
IgG antibody: Alk. Phos.RabbitDonkeyPolyclonal IgGC, E
IgG antibody: Alk. Phos.MonkeyGoatPolyclonal IgGE
IgG antibody: Alk. Phos.GoatRabbitPolyclonal IgGC, E, IB, WB
IgG antibody: Alk. Phos.ChickenRabbitPolyclonal IgGC, E, WB
IgG antibody: Alk. Phos.GoatDonkeyPolyclonal IgGC, E
IgG antibody: Alk. Phos.RatDonkeyPolyclonal IgGC, E, WB
IgG IgA IgM antibody: Alk. Phos.MouseGoatPolyclonal IgGC, E, WB
IgG IgM antibody: Alk. Phos.RabbitGoatPolyclonal IgGE
IgG1 antibody: Alk. Phos.RatGoatPolyclonal IgGE, WB
IgG1 antibody: Alk. Phos.MouseGoatPolyclonal IgGC, E, P, WB
IgG2 antibody: Alk. Phos.DogSheepPolyclonal IgGE
IgG2a antibody: Alk. Phos.RatGoatPolyclonal IgGE, WB
IgG2a antibody: Alk. Phos.MouseGoatPolyclonal IgGC, E, P, WB
IgM antibody: Alk. Phos.RabbitGoatPolyclonal IgGE
IgM antibody: Alk. Phos.RatGoatPolyclonal IgGE, WB
IgM antibody: Alk. Phos.SheepRabbitPolyclonal IgGE
IgM antibody: Alk. Phos.GoatRabbitPolyclonal IgGE
IgM antibody: Alk. Phos.MouseGoatPolyclonal IgGC, E, P, WB
IgM antibody: Alk. Phos.HumanGoatPolyclonal IgGC, E, IB
IgM antibody: Alk. Phos.PigGoatPolyclonal IgGE
IgM antibody: Alk. Phos.BovineSheepPolyclonal IgGE, WB
KAPPA LIGHT CHAIN antibody: Alk. Phos.MouseGoatPolyclonal IgGC, E, IB
KAPPA LIGHT CHAIN antibody: Alk. Phos.HumanGoatPolyclonal IgGC, E, IB
LAMBDA LIGHT CHAIN antibody: Alk. Phos.MouseGoatPolyclonal IgGE
IgM antibody: APCMouseGoatPolyclonal IgGE, F, IF
Ig antibody: BiotinHumanGoatPolyclonal IgGE, IB, IF
IgA antibody: BiotinBovineSheepPolyclonal IgGE, WB
IgA antibody: BiotinMonkeyMouseIgG1IgA5-3BE
IgA antibody: BiotinRatMouseIgG1MARA-1E
IgD antibody: BiotinRatMouseIgG1MARD-3E
IgD antibody: BiotinHumanGoatPolyclonal IgGE
IgE antibody: BiotinMouseRatIgG1LO-ME-3E
IgE antibody: BiotinDogMouseIgG1E6-71A1E, WB
IgE antibody: BiotinRatMouseIgG1MARE-1E, WB
IgG antibody: BiotinMouseGoatPolyclonal IgGE, IB, IF
IgG antibody: BiotinMouseRatCocktailCOCKTAILE
IgG antibody: BiotinRabbitSheepPolyclonal IgGC, E, P, WB
IgG antibody: BiotinHumanGoatPolyclonal IgGC, E, F, WB
IgG antibody: BiotinPigGoatPolyclonal IgGE, WB
IgG antibody: BiotinRatGoatPolyclonal IgGC, E, IF, P, WB
IgG antibody: BiotinRatDonkeyPolyclonal IgGC, E
IgG IgA IgM antibody: BiotinMouseGoatPolyclonal IgGE, IB
IgG1 antibody: BiotinBovineSheepPolyclonal IgGE, WB
IgG1 antibody: BiotinMouseRatIgG1LO-MG1-2E
IgG2a antibody: BiotinMouseGoatPolyclonal IgGE
IgG2a antibody: BiotinMouseRatIgG1LO-MG2a-7E
IgG2b antibody: BiotinMouseRatIgG1LO-MG2b-2E
IgG2b antibody: BiotinRatMouseIgG1MARG2b-3E
IgG3 antibody: BiotinMouseRatIgMLO-MG3-7E
IgM antibody: BiotinRatGoatPolyclonal IgGC, E, IB
IgM antibody: BiotinRatMouseIgG1MARM-4E
IgM antibody: BiotinBovineSheepPolyclonal IgGE, WB
KAPPA LIGHT CHAIN antibody: BiotinHumanGoatPolyclonal IgGE, IB, IF
KAPPA LIGHT CHAIN antibody: BiotinHumanGoatPolyclonal IgGE, IF, WB
LAMBDA LIGHT CHAIN antibody: BiotinHumanGoatPolyclonal IgGE, IB, IF
IgG antibody: ConcentrateHumanRabbitPolyclonal IgGC, P *
KAPPA LIGHT CHAIN antibody: ConcentrateHumanRabbitPolyclonal IgGC, P *
IgG antibody: DyLight®405RatRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®405MouseGoatPolyclonal IgGF, IF
IgG antibody: DyLight®405HamsterGoatPolyclonal IgGF, IF
IgG antibody: DyLight®405MouseRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®488HamsterGoatPolyclonal IgGF, IF
IgG antibody: DyLight®488SheepDonkeyPolyclonal IgGF, IF
IgG antibody: DyLight®488RatRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®488MouseRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®488MouseGoatPolyclonal IgGF, IF
IgG antibody: DyLight®488RabbitSheepPolyclonal IgGF, IF
IgG antibody: DyLight®549MouseRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®549RatGoatPolyclonal IgGF, IF
IgG antibody: DyLight®549MouseGoatPolyclonal IgGF, IF
IgG antibody: DyLight®649HamsterGoatPolyclonal IgGF, IF
IgG antibody: DyLight®649MouseGoatPolyclonal IgGF, IF
IgG antibody: DyLight®649RatGoatPolyclonal IgGF, IF
IgG antibody: DyLight®649MouseRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®649SheepDonkeyPolyclonal IgGF, IF
IgG antibody: DyLight®649RatRabbitPolyclonal IgGF, IF
IgG antibody: DyLight®800MouseRabbitPolyclonal IgGF, IF, WB
IgG antibody: DyLight®800RatRabbitPolyclonal IgGF, IF, WB
IgG antibody: DyLight®800MouseGoatPolyclonal IgGF, IF, WB
IgA antibody: FITCBovineSheepPolyclonal IgGC, F
IgA antibody: FITCRabbitGoatPolyclonal IgGC, F
IgA antibody: FITCHumanMouseIgG115D6E, IF
IgA antibody: FITCDogGoatPolyclonal IgGC, F
IgA antibody: FITCSheepRabbitPolyclonal IgGF
IgA antibody: FITCHumanGoatPolyclonal IgGF, IF, P
IgA antibody: FITCHumanGoatPolyclonal IgGC, F, IF
IgD antibody: FITCHumanGoatPolyclonal IgGC, F, IF
IgE antibody: FITCRatMouseIgG1MARE-1F
IgE antibody: FITCHumanGoatPolyclonal IgGF
IgG antibody: FITCMouseRabbitPolyclonal IgGF
IgG antibody: FITCCatGoatPolyclonal IgGC, F
IgG antibody: FITCRabbitSheepPolyclonal IgGC, F
IgG antibody: FITCRatGoatPolyclonal IgGF
IgG antibody: FITCHumanGoatPolyclonal IgGC, F
IgG antibody: FITCHumanGoatPolyclonal IgGC, F, IF
IgG antibody: FITCDogSheepPolyclonal IgGC, F, IF
IgG antibody: FITCHamsterGoatPolyclonal IgGF
IgG antibody: FITCHumanGoatPolyclonal IgGC, F, IF
IgG antibody: FITCPigGoatPolyclonal IgGC, F
IgG antibody: FITCRabbitPolyclonal IgGF
IgG antibody: FITCRatGoatPolyclonal IgGF
IgG antibody: FITCHamsterGoatPolyclonal IgGF
IgG antibody: FITCMonkeyGoatPolyclonal IgGC, F, IC, IF
IgG antibody: FITCHumanGoatPolyclonal IgGC, F
IgG antibody: FITCGuinea PigGoatPolyclonal IgGC, F
IgG antibody: FITCRatDonkeyPolyclonal IgGIF
IgG antibody: FITCRabbitGoatPolyclonal IgGF, IF
IgG antibody: FITCGoatDonkeyPolyclonal IgGIF
IgG antibody: FITCChickenGoatPolyclonal IgGF
IgG antibody: FITCGoatRabbitPolyclonal IgGF
IgG antibody: FITCMouseGoatPolyclonal IgGF
IgG antibody: FITCPigGoatPolyclonal IgGC, F
IgG IgA IgM antibody: FITCMouseGoatPolyclonal IgGF
IgG IgA IgM antibody: FITCHumanGoatPolyclonal IgGF
IgG IgM antibody: FITCRabbitGoatPolyclonal IgGF
IgG1 antibody: FITCRatGoatPolyclonal IgGF
IgG1 antibody: FITCDogGoatPolyclonal IgGF
IgG2a antibody: FITCRatGoatPolyclonal IgGF
IgG2a antibody: FITCMouseGoatPolyclonal IgGC, F, IF
IgG2a antibody: FITCMouseRabbitPolyclonal IgGIF
IgG2a antibody: FITCMouseGoatPolyclonal IgGC, F
IgG2a antibody: FITCRatMouseIgG1MARG2a-1F
IgG2b antibody: FITCRatMouseIgG1MARG2b-3F
IgG2b antibody: FITCMouseGoatPolyclonal IgGC, F, IF
IgG2b antibody: FITCMouseHuCALHuCAL Fab bivalent4642F
IgG2c antibody: FITCRatHuCALHuCAL Fab bivalent9337F
IgG2c antibody: FITCMouseGoatPolyclonal IgGF
IgG3 antibody: FITCMouseGoatPolyclonal IgGC, F, IF
IgG3 antibody: FITCMouseHuCALHuCAL Fab bivalent4582F
IgG3 antibody: FITCHumanSheepPolyclonal IgGIF
IgM antibody: FITCChickenGoatPolyclonal IgGC, F
IgM antibody: FITCBovineSheepPolyclonal IgGC, F
IgM antibody: FITCHumanGoatPolyclonal IgGC, F, IF
IgM antibody: FITCMouseHuCALHuCAL Fab bivalent4620F
IgM antibody: FITCCatGoatPolyclonal IgGC, F
IgM antibody: FITCRabbitGoatPolyclonal IgGF
IgM antibody: FITCGoatRabbitPolyclonal IgGC, F
IgM antibody: FITCHumanGoatPolyclonal IgGC, F, IF
IgM antibody: FITCMouseGoatPolyclonal IgGIF
KAPPA LIGHT CHAIN antibody: FITCMouseGoatPolyclonal IgGF
KAPPA LIGHT CHAIN antibody: FITCMouseRatIgG2bOX-20F
KAPPA LIGHT CHAIN antibody: FITCHumanGoatPolyclonal IgGC, F, IF
KAPPA LIGHT CHAIN antibody: FITCHumanGoatPolyclonal IgGC, F
Ig antibody: HRPRatGoatPolyclonal IgGC, E, IB
IgA antibody: HRPBovineMouseIgG2aIL-A71E
IgA antibody: HRPDogGoatPolyclonal IgGC, E
IgA antibody: HRPRatGoatPolyclonal IgGE, WB
IgA antibody: HRPChickenGoatPolyclonal IgGE
IgA antibody: HRPMouseGoatPolyclonal IgGC, E, P, WB
IgA antibody: HRPHumanGoatPolyclonal IgGC, E, WB
IgA antibody: HRPHumanGoatPolyclonal IgGC, E
IgA1 antibody: HRPHumanMouseIgG1B3506B4E
IgE antibody: HRPHumanMouseIgG2aE454 (5D5)E
IgE antibody: HRPMouseGoatPolyclonal IgGE
IgE antibody: HRPDogGoatPolyclonal IgGC, E, WB
IgE antibody: HRPHumanGoatPolyclonal IgGE
IgE antibody: HRPHumanMouseIgG11A2E
IgE antibody: HRPMouseHuCALHuCAL Fab bivalent4658E
IgG antibody: HRPMouseGoatPolyclonal IgGC, E, P
IgG antibody: HRPRabbitGoatPolyclonal IgGE
IgG antibody: HRPRabbitSheepPolyclonal IgGC, E, WB
IgG antibody: HRPHumanGoatPolyclonal IgGE, WB
IgG antibody: HRPRhesus MonkeyHuCALHuCAL Fab bivalent8717E
IgG antibody: HRPHumanGoatPolyclonal IgGC, E, WB
IgG antibody: HRPPigGoatPolyclonal IgGE
IgG antibody: HRPSheepDonkeyPolyclonal IgGC, E, P, WB
IgG antibody: HRPHumanGoatPolyclonal IgGC, E, IB
IgG antibody: HRPRabbitGoatPolyclonal IgGC, E, WB
IgG antibody: HRPDogSheepPolyclonal IgGE
IgG antibody: HRPHumanHuCALHuCAL Fab monovalent5985E
IgG antibody: HRPRatRabbitPolyclonal IgGC, E, P, RE
IgG antibody: HRPMouseGoatPolyclonal IgGWB
IgG antibody: HRPRabbitGoatPolyclonal IgGWB
IgG antibody: HRPMouseGoatPolyclonal IgGE
IgG antibody: HRPPigGoatPolyclonal IgGE
IgG antibody: HRPChickenGoatPolyclonal IgGE
IgG antibody: HRPRabbitGoatPolyclonal IgGC, E, WB
IgG antibody: HRPMouseGoatPolyclonal IgGC, E, WB
IgG antibody: HRPRatGoatPolyclonal IgGC, E, IB
IgG antibody: HRPGuinea PigGoatPolyclonal IgGE
IgG antibody: HRPGoatRabbitPolyclonal IgGC, E, WB
IgG antibody: HRPHumanGoatPolyclonal IgGC, E, WB
IgG antibody: HRPHumanMouseIgG1MK 1 A6C *, E
IgG antibody: HRPSheepRabbitPolyclonal IgGC, E, WB
IgG antibody: HRPMouseGoatPolyclonal IgGE, WB
IgG antibody: HRPMouseSheepPolyclonal IgGC *, E, WB
IgG antibody: HRPHumanGoatPolyclonal IgGC, E
IgG antibody: HRPMonkeyGoatPolyclonal IgGE
IgG antibody: HRPGoatDonkeyPolyclonal IgGC, E
IgG antibody: HRPRabbitDonkeyPolyclonal IgGC, E
IgG antibody: HRPRabbitGoatPolyclonal IgGC, E, WB
IgG antibody: HRPMouseGoatPolyclonal IgGC, E, WB
IgG IgA IgM antibody: HRPMouseGoatPolyclonal IgGE
IgG IgA IgM antibody: HRPHumanGoatPolyclonal IgGE, WB
IgG IgM antibody: HRPRabbitGoatPolyclonal IgGE
IgG1 antibody: HRPRatGoatPolyclonal IgGE, WB
IgG1 antibody: HRPBovineMouseIgG1IL-A60E
IgG1 antibody: HRPMouseHuCALHuCAL Fab bivalent4639E
IgG1 antibody: HRPRatMouseIgG1MARG1-2E
IgG1 antibody: HRPDogGoatPolyclonal IgGC, E, WB
IgG1 antibody: HRPMouseGoatPolyclonal IgGC, E, P, WB
IgG1 antibody: HRPMouseRatIgG1LO-MG1-2E
IgG1 antibody: HRPRatHuCALHuCAL Fab bivalent8938E
IgG2 antibody: HRPBovineMouseIgG1IL-A73E
IgG2 antibody: HRPDogSheepPolyclonal IgGC, E, WB
IgG2a antibody: HRPRatMouseIgG1MARG2a-1E
IgG2a antibody: HRPRatGoatPolyclonal IgGE, WB
IgG2a antibody: HRPMouseGoatPolyclonal IgGC, E, P, WB
IgG2a antibody: HRPRatHuCALHuCAL Fab bivalent9345E
IgG2b antibody: HRPRatGoatPolyclonal IgGC, E, P, WB
IgG2b antibody: HRPMouseGoatPolyclonal IgGC, E, P, WB
IgG2b antibody: HRPRatHuCALHuCAL Fab bivalent9204E
IgG3 antibody: HRPHumanMouseIgG1HP6050C, E, WB
IgG3 antibody: HRPMouseGoatPolyclonal IgGC, E, P, WB
IgG3 antibody: HRPHumanSheepPolyclonal IgGC, E
IgG4 antibody: HRPHumanMouseIgG1HP6025C, E, P
IgGc antibody: HRPHorseGoatPolyclonal IgGE, WB
IgM antibody: HRPBovineSheepPolyclonal IgGE
IgM antibody: HRPMouseHuCALHuCAL Fab bivalent4620E
IgM antibody: HRPChickenGoatPolyclonal IgGE
IgM antibody: HRPRatMouseIgG1MARM-4E
IgM antibody: HRPMouseGoatPolyclonal IgGC, E, P
IgM antibody: HRPDogGoatPolyclonal IgGE
IgM antibody: HRPHumanGoatPolyclonal IgGC, E, P, WB
IgM antibody: HRPRatGoatPolyclonal IgGC, E, IB
IgM antibody: HRPBovineMouseIgG1IL-A30E
IgM antibody: HRPGoatRabbitPolyclonal IgGE
KAPPA LIGHT CHAIN antibody: HRPMouseGoatPolyclonal IgGC, E, IB
KAPPA LIGHT CHAIN antibody: HRPHumanGoatPolyclonal IgGC, E, P, WB
KAPPA LIGHT CHAIN antibody: HRPRatMouseIgG2aOX-12E
LAMBDA LIGHT CHAIN antibody: HRPMouseGoatPolyclonal IgGE
LAMBDA LIGHT CHAIN antibody: HRPHumanGoatPolyclonal IgGC, E, WB
IgE antibody: KitMouseE
ALKALINE PHOSPHATASE antibody: PurifiedHumanE
BETA 2 MICROGLOBULIN antibody: PurifiedHumanE, WB
BETA 2 MICROGLOBULIN antibody: PurifiedHumanE, WB
C1q antibody: PurifiedHumanE
C3 antibody: PurifiedHumanE
C3b antibody: PurifiedHumanE
C4c antibody: PurifiedHumanE
CD66e antibody: PurifiedHumanE
HAPTOGLOBIN antibody: PurifiedHumanE
Ig antibody: PurifiedHumanGoatPolyclonal IgGE, IB
Ig antibody: PurifiedRatGoatPolyclonal IgGE
IgA antibody: PurifiedBovineSheepPolyclonal IgGE, ID
IgA antibody: PurifiedPigGoatPolyclonal IgGE, ID
IgA antibody: PurifiedMouseRatIgMLO-MA-10E
IgA antibody: PurifiedMouseGoatPolyclonal IgGC, E
IgA antibody: PurifiedBovineMouseIgG2aIL-A71E, IP
IgA antibody: PurifiedCatMouseIgG1IgA9-6AE, WB
IgA antibody: PurifiedSheepRabbitPolyclonal IgGE
IgA antibody: PurifiedHumanGoatPolyclonal IgGC, E, WB
IgA antibody: PurifiedHumanMouseIgG115D6E
IgA antibody: PurifiedHorseGoatPolyclonal IgGE
IgA antibody: PurifiedMouseGoatPolyclonal IgGE
IgA1 antibody: PurifiedHumanMouseIgG114A/3C10E
IgA2 antibody: PurifiedHumanMouseIgG114AS (14A)E
IgA2 antibody: PurifiedHumanMouseIgG1A9604D2C, E, IB, WB
IgD antibody: PurifiedMouseRatIgG2aLO-MD-6E
IgD antibody: PurifiedRatMouseIgG1MARD-3C, E
IgD antibody: PurifiedHumanGoatPolyclonal IgGE
IgD antibody: PurifiedHumanMouseIgG2aIADB6E
IgD antibody: PurifiedHumanMouseIgG1LE2C
IgE antibody: PurifiedMouseGoatPolyclonal IgGE
IgE antibody: PurifiedMouseIgEE
IgE antibody: PurifiedMouseGoatPolyclonal IgGE, WB
IgE antibody: PurifiedDogMouseIgG2aE6-2A1E, WB
IgE antibody: PurifiedMouseRatIgG2aLO-ME-2E
IgE antibody: PurifiedHumanMouseIgG14C3E, FN*, WB
IgE antibody: PurifiedMouseRatIgG1LO-ME-3E
IgE antibody: PurifiedMouseHuCALHuCAL Fab bivalent4658E
IgE antibody: PurifiedRatIgEE
IgE antibody: PurifiedDogGoatPolyclonal IgGE, WB
IgE antibody: PurifiedDogMouseIgG1E6-71A1E, WB
IgE antibody: PurifiedHumanMouseIgG2aE411 (5H2)E, F
IgE antibody: PurifiedMouseSheepPolyclonal IgGID
IgG antibody: PurifiedHumanGoatPolyclonal IgGE, IF, WB
IgG antibody: PurifiedHumanGoatPolyclonal IgGE
IgG antibody: PurifiedRabbitPolyclonal IgGE
IgG antibody: PurifiedHamsterPolyclonal IgGE
IgG antibody: PurifiedBovineSheepPolyclonal IgGE, ID
IgG antibody: PurifiedBovineGoatPolyclonal IgGE, IF, WB
IgG antibody: PurifiedRatGoatPolyclonal IgGE, IF
IgG antibody: PurifiedHumanE
IgG antibody: PurifiedHumanGoatPolyclonal IgGE, IF, WB
IgG antibody: PurifiedHorseE, ID
IgG antibody: PurifiedHumanSheepPolyclonal IgGID
IgG antibody: PurifiedCatGoatPolyclonal IgGE, ID
IgG antibody: PurifiedMouseGoatPolyclonal IgGE, IF, WB
IgG antibody: PurifiedHumanHuCALHuCAL Fab bivalent6864E
IgG antibody: PurifiedRatE
IgG antibody: PurifiedMouseGoatPolyclonal IgGE
IgG antibody: PurifiedMouseGoatPolyclonal IgGE
IgG antibody: PurifiedHumanMouseIgG1R10Z8E9E
IgG antibody: PurifiedSheepE
IgG antibody: PurifiedHumanHuCALHuCAL Fab monovalent5985E
IgG antibody: PurifiedMouseRabbitPolyclonal IgGE, IF, WB
IgG antibody: PurifiedMouseRatCocktailCOCKTAILE
IgG antibody: PurifiedHumanGoatPolyclonal IgGE
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE, WB
IgG antibody: PurifiedDogIgGN/AE
IgG antibody: PurifiedRatGoatPolyclonal IgGE, IF
IgG antibody: PurifiedCatMouseIgG1GPB2-2B1E, WB
IgG antibody: PurifiedHumanGoatPolyclonal IgGE
IgG antibody: PurifiedBovineE
IgG antibody: PurifiedGoatRabbitPolyclonal IgGE
IgG antibody: PurifiedRhesus MonkeyHuCALHuCAL Fab bivalent8717E
IgG antibody: PurifiedMouseGoatPolyclonal IgGE, IB
IgG antibody: PurifiedHumanGoatPolyclonal IgGE, IB, IF
IgG antibody: PurifiedHumanHuCALHuCAL Fab bivalent6863E
IgG antibody: PurifiedMouseGoatPolyclonal IgGE, F
IgG antibody: PurifiedRabbitRatIgG2aLO-RG-1E, IF
IgG antibody: PurifiedPigE
IgG antibody: PurifiedGoatRabbitPolyclonal IgGE, IF, WB
IgG antibody: PurifiedHumanMouseIgG1SB2H2E
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE, IB
IgG antibody: PurifiedMouseIY
IgG antibody: PurifiedRatGoatPolyclonal IgGE, IP
IgG antibody: PurifiedHumanRabbitPolyclonal IgGE, IF, WB
IgG antibody: PurifiedDogSheepPolyclonal IgGE, ID
IgG antibody: PurifiedRhesus MonkeyHuCALHuCAL Fab bivalent8718E
IgG antibody: PurifiedHumanGoatPolyclonal IgGC, E
IgG antibody: PurifiedGuinea PigGoatPolyclonal IgGE
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE, WB
IgG antibody: PurifiedSheepRabbitPolyclonal IgGE, ID, IP, WB
IgG antibody: PurifiedHumanGoatPolyclonal IgGE, IP, WB
IgG antibody: PurifiedMouseGoatPolyclonal IgGE
IgG antibody: PurifiedRabbitGoatPolyclonal IgGC, E, WB
IgG antibody: PurifiedChickenGoatPolyclonal IgGE, ID
IgG antibody: PurifiedRabbitDonkeyPolyclonal IgGC, E
IgG antibody: PurifiedHumanMouseIgMA57HC, E, H, P, WB
IgG antibody: PurifiedRatRabbitPolyclonal IgGE
IgG antibody: PurifiedGoatRabbitPolyclonal IgGC, E
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE, IB
IgG antibody: PurifiedHorseGoatPolyclonal IgGE, ID
IgG antibody: PurifiedMouseGoatPolyclonal IgGE, F, WB
IgG antibody: PurifiedMouseRabbitPolyclonal IgGC, E, IF, WB
IgG antibody: PurifiedHumanGoatPolyclonal IgGC, E, ID, WB
IgG antibody: PurifiedGoatDonkeyPolyclonal IgGC, E
IgG antibody: PurifiedGoatRabbitPolyclonal IgGE
IgG antibody: PurifiedHumanGoatPolyclonal IgGE, WB
IgG antibody: PurifiedRabbitGoatPolyclonal IgGE
IgG antibody: PurifiedMouseGoatPolyclonal IgGC, E
IgG antibody: PurifiedMouseGoatPolyclonal IgGE, WB
IgG antibody: PurifiedRatGoatPolyclonal IgGC, E, IB, IF
IgG antibody: PurifiedRatGoatPolyclonal IgGE, IP
IgG antibody: PurifiedSheepDonkeyPolyclonal IgGE, IP
IgG IgA IgM antibody: PurifiedHumanGoatPolyclonal IgGE
IgG IgA IgM antibody: PurifiedMouseGoatPolyclonal IgGC, E
IgG IgA IgM antibody: PurifiedMouseGoatPolyclonal IgGE, F, IB
IgG IgM antibody: PurifiedRabbitGoatPolyclonal IgGE, IB
IgG1 antibody: PurifiedMouseRatIgG1LO-MG1-2E
IgG1 antibody: PurifiedMouseIgG1E
IgG1 antibody: PurifiedMouseIgG1MADNP-1E, GS
IgG1 antibody: PurifiedDogGoatPolyclonal IgGC, E, WB
IgG1 antibody: PurifiedHumanE, FN*
IgG1 antibody: PurifiedHumanSheepPolyclonal IgGE, WB
IgG1 antibody: PurifiedRatMouseIgG1MARG1-2E
IgG1 antibody: PurifiedMouseE
IgG1 antibody: PurifiedRatHuCALHuCAL Fab bivalent8941E
IgG1 antibody: PurifiedMouseGoatPolyclonal IgGE
IgG1 antibody: PurifiedMouseHuCALHuCAL Fab bivalent4639E
IgG1 antibody: PurifiedMouseGoatPolyclonal IgGE, IF, WB
IgG1 antibody: PurifiedRatHuCALHuCAL Fab bivalent8938E
IgG1 antibody: PurifiedHumanMouseIgG1NL 16C *, E, WB
IgG1 antibody: PurifiedHumanMouseIgG2a8c/6-39 (HP6091)C *, ID
IgG2 antibody: PurifiedBovineMouseIgG1IL-A73E, F, IP
IgG2 antibody: PurifiedBovineSheepPolyclonal IgGE, ID
IgG2 antibody: PurifiedDogSheepPolyclonal IgGC, E, WB
IgG2 antibody: PurifiedHumanE
IgG2 antibody: PurifiedHumanMouseIgG1HP6200C *, E, R, WB
IgG2 antibody: PurifiedHumanE
IgG2a antibody: PurifiedMouseRatIgG1LO-MG2a-7E
IgG2a antibody: PurifiedRatMouseIgG1MARG2a-1E
IgG2a antibody: PurifiedRatHuCALHuCAL Fab bivalent9345E
IgG2a antibody: PurifiedRatIgG2aE
IgG2a antibody: PurifiedRatGoatPolyclonal IgGE, F, WB
IgG2b antibody: PurifiedMouseRatIgG1LO-MG2b-2E
IgG2b antibody: PurifiedRatMouseIgG1MARG2b-8E
IgG2b antibody: PurifiedRatIR863E
IgG2b antibody: PurifiedMouseHuCALHuCAL Fab bivalent4642E, F
IgG2b antibody: PurifiedRatHuCALHuCAL Fab bivalent9204E
IgG2c antibody: PurifiedMouseGoatPolyclonal IgGE
IgG2c antibody: PurifiedRatMouseIgG2aMARG2c-5E, F
IgG2c antibody: PurifiedRatHuCALHuCAL Fab bivalent9337E, F
IgG3 antibody: PurifiedMouseHuCALHuCAL Fab bivalent4582E, F
IgG3 antibody: PurifiedHumanMouseIgG1HP6050C, E, F
IgG3 antibody: PurifiedMouseRatIgG1LO-MG3-13E
IgG3 antibody: PurifiedHumanIgG3ID
IgG4 antibody: PurifiedHumanE
IgG4 antibody: PurifiedHumanMouseIgG1HP6025C, IB, P, WB
IgGa antibody: PurifiedHorseMouseIgG1CVS48E, F
IgM antibody: PurifiedBovineSheepPolyclonal IgGE, ID
IgM antibody: PurifiedMouseHuCALHuCAL Fab bivalent10922E
IgM antibody: PurifiedBovineMouseIgG1IL-A30E, F, IP, R
IgM antibody: PurifiedRatGoatPolyclonal IgGC, E, WB
IgM antibody: PurifiedMouseHuCALHuCAL Fab bivalent4620E, F
IgM antibody: PurifiedPigIgME
IgM antibody: PurifiedHumanMouseIgG147.2.E9E
IgM antibody: PurifiedHumanIgME
IgM antibody: PurifiedMouseRatIgMLO-MM-3E
IgM antibody: PurifiedChickenGoatPolyclonal IgGE, ID
IgM antibody: PurifiedMouseGoatPolyclonal IgGC, E, WB
IgM antibody: PurifiedHorseGoatPolyclonal IgGE, ID
IgM antibody: PurifiedSheepMouseIgG125.69F
IgM antibody: PurifiedMouseRatIgG2aLO-MM-9C, E
IgM antibody: PurifiedHumanMouseIgG1CM7E, F, P, WB
IgM antibody: PurifiedSheepRabbitPolyclonal IgGE
IgM antibody: PurifiedSheepMouseIgG2bVPM13C, E, F, IP
IgM antibody: PurifiedHumanE
IgM antibody: PurifiedDogGoatPolyclonal IgGC, E, F
IgM antibody: PurifiedRatIR473E
IgM antibody: PurifiedHumanGoatPolyclonal IgGE
IgM antibody: PurifiedCatGoatPolyclonal IgGE
IgM antibody: PurifiedMouseIgMMADNP-5E
KAPPA LIGHT CHAIN antibody: PurifiedHumanGoatPolyclonal IgGE
KAPPA LIGHT CHAIN antibody: PurifiedMouseGoatPolyclonal IgGE, F, IP
KAPPA LIGHT CHAIN antibody: PurifiedHumanMouseIgG2a4G7E
KAPPA LIGHT CHAIN antibody: PurifiedHumanGoatPolyclonal IgGE
LAMBDA LIGHT CHAIN antibody: PurifiedRatMouseIgG1MARL-15C, E, WB
LAMBDA LIGHT CHAIN antibody: PurifiedSheepMouseIgG1VPM8C, E, F, IP, WB
LAMBDA LIGHT CHAIN antibody: PurifiedMouseGoatPolyclonal IgGE, IB, IF, WB
LAMBDA LIGHT CHAIN antibody: PurifiedHumanGoatPolyclonal IgGE
PROTEIN S antibody: PurifiedHumanE
KAPPA LIGHT CHAIN antibody: Ready To UseHumanRabbitPolyclonal IgGC, P *
IgD antibody: RPEHumanGoatPolyclonal IgGE, F
IgG antibody: RPEMouseGoatPolyclonal IgGF
IgG antibody: RPERabbitSheepPolyclonal IgGF
IgG antibody: RPERatGoatPolyclonal IgGF
IgG antibody: RPEHumanGoatPolyclonal IgGF
IgG antibody: RPERatRabbitPolyclonal IgGF
IgG antibody: RPEMouseRabbitPolyclonal IgGF
IgG antibody: RPERatGoatPolyclonal IgGF
IgG antibody: RPERatDonkeyPolyclonal IgGF
IgG antibody: RPEGoatDonkeyPolyclonal IgGF
IgG antibody: RPEHumanGoatPolyclonal IgGF
IgG antibody: RPEMouseGoatPolyclonal IgGF
IgG IgM antibody: RPERabbitGoatPolyclonal IgGF
IgG1 antibody: RPEMouseGoatPolyclonal IgGF
IgG2b antibody: RPEMouseGoatPolyclonal IgGF
IgG3 antibody: RPEMouseHuCALHuCAL Fab bivalent4582F
IgM antibody: RPEHumanGoatPolyclonal IgGF
IgM antibody: RPEHumanGoatPolyclonal IgGF
IgM antibody: RPEMouseGoatPolyclonal IgGF
KAPPA LIGHT CHAIN antibody: RPEHumanGoatPolyclonal IgGF
KAPPA LIGHT CHAIN antibody: RPEHumanGoatPolyclonal IgGF
LAMBDA LIGHT CHAIN antibody: RPEHumanMouseIgG1Mc24-IC6F
LAMBDA LIGHT CHAIN antibody: RPEHumanGoatPolyclonal IgGF
IgA antibody: S/NCatMouseIgG1K218 3E10E
IgA antibody: S/NBovineMouseIgG1K84 2F9E
IgA antibody: S/NPigMouseIgG1K60 1F1C, E
IgG antibody: S/NCatMouseIgG1K62 2G4E
IgG IgA IgM antibody: S/NDogMouseIgG1K99 2E3E
IgG IgA IgM antibody: S/NCatMouseIgG1K185 4D4E
IgG IgA IgM antibody: S/NBovineMouseIgG1K67 3G2E
IgG2 antibody: S/NBovineMouseIgG1K192 4F10E
IgM antibody: S/NPigMouseIgG1K52 1C3C, E, F
LAMBDA LIGHT CHAIN antibody: S/NPigMouseIgG2aK139 3E1C, E, F, P
IgG antibody: SerumRabbitSheepPolyclonal IgGE
IgG antibody: SerumRatGoatPolyclonal IgGR
IgG antibody: SerumChickenSheepPolyclonal IgGIP
IgM antibody: SerumHumanSheepPolyclonal IgGE, ID, IP
LAMBDA LIGHT CHAIN antibody: SerumHumanGoatPolyclonal IgGTB
Ig antibody: Texas Red®RatGoatPolyclonal IgGF
IgG IgM antibody: Texas Red®RabbitGoatPolyclonal IgGF
IgG2b antibody: Texas Red®MouseGoatPolyclonal IgGF
IgM antibody: Texas Red®HumanGoatPolyclonal IgGF
KAPPA LIGHT CHAIN antibody: Texas Red®MouseGoatPolyclonal IgGF
Ig antibody: TRITCHumanGoatPolyclonal IgGC, F
IgG antibody: TRITCRatGoatPolyclonal IgGC, F
IgG antibody: TRITCRatGoatPolyclonal IgGF, IF
IgM antibody: TRITCRatGoatPolyclonal IgGIF
IgM antibody: TRITCHumanGoatPolyclonal IgGC, F
LAMBDA LIGHT CHAIN antibody: TRITCHumanGoatPolyclonal IgGC, F

Horseradish-peroxidase conjugated antibodies are preferentially used whenever an indirect method is used for detection on Western Blots. AbD Serotec supplies a range of species specific antibodies recognizing immunoglobulin or any of its isotypes. Monoclonal and polyclonal secondary antibodies are available, as a whole IgG or a F(ab’)2 fragment. Performance is further enhanced by affinity purification for all STAR reagents and many anti-mouse, -rat, and -hamster secondary antibodies are multi-species adsorbed.


LI-COR Odyssey® is a registered trademark of LI-COR Biosciences, Inc. USA.
AbGuard® is a registered trademark of MorphoSys UK Ltd