CD54 antibody | 15.2
Mouse anti Human CD54
- Product Type
- Monoclonal Antibody
- Clone
- 15.2
- Isotype
- IgG1
- Specificity
- CD54
Mouse anti Human CD54 antibody, clone 15.2 recognizes the human CD54 cell surface antigen also known as intracellular Adhesion Molecule -1 (ICAM-1) or Major group rhinovirus receptor. CD54 is expressed by many cells following activation by inflammatory mediators. It is a 505 amino acid with an additional 27 amino acid signal peptide ~90 kDa single pass type I transmembrane glycoprotein bearing 5 Ig-like C2-type domains. Mouse anti Human CD54 antibody, clone 15.2 blocks CD54 function (Berendt et al. 1992). Mouse anti Human CD54 antibody, clone 15.2 binds to an epitope on the N-terminal Ig-like domain within a region designated the L43 loop (Chakravorty and Craig 2005). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- MCA1615, MCA1615T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA1615GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human monocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0-Ag14 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/100 | |
Immunohistology - Frozen | 1/50 | 1/100 | |
Immunohistology - Paraffin | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Histology Positive Control Tissue
- Human tonsil
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control |
References for CD54 antibody
-
Dransfield, I. et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.
J Cell Biol. 116:1527-35. -
Berendt, A. et al. (1992) The binding site on ICAM-1 for plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1- binding site.
Cell. 68: 71-81. -
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321 (2): 656-62. -
Baratin, M. et al. (2007) Dissection of the role of PfEMP1 and ICAM-1 in the sensing of Plasmodium-falciparum-infected erythrocytes by natural killer cells.
PLos One 2: e228. -
van Buul, J.D. et al. (2010) Inside-out regulation of ICAM-1 dynamics in TNF-alpha-activated endothelium.
PLoS One 5: e11336. -
Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
J Cell Physiol. 214: 75-83. -
Di Lorenzo, A. et al. (2011) Endothelial reticulon-4B (Nogo-B) regulates ICAM-1-mediated leukocyte transmigration and acute inflammation.
Blood. 117: 2284-95. -
Porter, J.C. and Hall, A. (2009) Epithelial ICAM-1 and ICAM-2 regulate the egression of human T cells across the bronchial epithelium.
FASEB J. 23: 492-502.
View The Latest Product References
-
Corvaisier, M. et al. (2005) V gamma 9V delta 2 T cell response to colon carcinoma cells.
J Immunol. 175: 5481-8. -
Horrocks, P. et al. (2005) PfEMP1 expression is reduced on the surface of knobless Plasmodium falciparum infected erythrocytes.
J Cell Sci. 118: 2507-18. -
Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.
J Immunol. 181: 6109-16. -
Norling, L.V. et al. (2008) Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking.
FASEB J. 22 (3): 682-90. -
Baumer, Y. et al. (2011) Telomerase-based immortalization modifies the angiogenic/inflammatory responses of human coronary artery endothelial cells.
Exp Biol Med (Maywood). 236: 692-700. -
Lask, A. et al. (2011) TCR-independent killing of B cell malignancies by anti-third-party CTLs: the critical role of MHC-CD8 engagement.
J Immunol. 187 (4): 2006-14. -
Sommaggio, R. et al. (2012) Multiple Receptors Trigger Human NK Cell-Mediated Cytotoxicity against Porcine Chondrocytes.
J Immunol. 188: 2075-83. -
Murphy, A.J. et al. (2013) Anti-inflammatory functions of apolipoprotein a-I and high-density lipoprotein are preserved in trimeric apolipoprotein a-I.
J Pharmacol Exp Ther. 344: 41-9. -
Sumagin R et al. (2014) Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.
Mucosal Immunol. 7 (4): 905-15. -
Sugden SM et al. (2017) HIV-1 Vpu Downmodulates ICAM-1 Expression, Resulting in Decreased Killing of Infected CD4+ T Cells by NK Cells.
J Virol. 91 (8): pii: e02442-16. -
Lennartz, F. et al. (2015) Mapping the Binding Site of a Cross-Reactive Plasmodium falciparum PfEMP1 Monoclonal Antibody Inhibitory of ICAM-1 Binding.
J Immunol. 195 (7): 3273-83. -
Schoppmeyer, R. et al. (2022) The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner.
Cell Rep. 38 (3): 110243. -
Grönloh, M.L.B. et al. (2023) Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM-1.
EMBO Rep. 24 (1): e55483. -
Grönloh, M.L.B. et al. (2023) Intercellular adhesion molecule 2 regulates diapedesis hotspots by allowing neutrophil crawling against the direction of flow.
Vasc Biol. 5 (1): e230005.
- Synonyms
- ICAM-1
- RRID
- AB_321783
- UniProt
- P05362
- Entrez Gene
- ICAM1
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0001910 regulation of leukocyte mediated cytotoxicity
- GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
- GO:0002693 positive regulation of cellular extravasation
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0007159 leukocyte cell-cell adhesion
- View More GO Terms
- GO:0022614 membrane to membrane docking
- GO:0046813 virion attachment, binding of host cell surface receptor
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
- GO:0051856 adhesion to symbiont
- GO:0060333 interferon-gamma-mediated signaling pathway
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