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Histidine Rich Protein II Antibody | 14964

Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium Falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from Plasmodium falciparum) was titrated using  the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium FalciparumHRPII (HCA159) antibody

Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium Falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from Plasmodium falciparum) was titrated using  the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium FalciparumHRPII (HCA159) antibody

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  • Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium Falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from Plasmodium falciparum) was titrated using  the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium FalciparumHRPII (HCA159) antibody
  • Human anti Plasmodium falciparum HRPII:HRP
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  • Product Type
    Monoclonal Antibody
  • Clone
    14964
  • Isotype
    IgG1
1 Format Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA159PE0.1 mg
    HCA159P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Plasmodium falciparum HRPII antibody, clone 14964 recognizes HRPII (Histidine Rich Protein II) from the Malaria causing protozoan species Plasmodium falciparum .


      Clone 14964 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.

      When conjugated this clone can be used as a detection antibody with HCA160 as the capture antibody in sandwich assay.
    • Intended Use
    • Target Species
      Protozoan
    • Product Form
      Human IgG1 antibody selected from the HuCAL ® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A.
    • Preservative Stabilisers
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
    • Immunogen
      Recombinant HRPII fusion protein from Plasmodium falciparum
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      Please view the datasheet pdf for suggested dilutions.
    • Technical Advice
      Protocols and further information may be found in the HuCAL Technical Manual
    • Recommended Protocol
    • ELISA
      This product is suitable for use in direct ELISA applications. This product may be used as a detection reagent in a sandwich ELISA together with HCA160 as the capture reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Histidine Rich Protein II Antibody Formats

    FormatsCloneApplicationsSizes available
    Histidine Rich Protein II Antibody : HRP14964E0.1 mg
    • Copyright © 2015 Bio-Rad AbD Serotec

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium Falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from Plasmodium falciparum) was titrated using  the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium FalciparumHRPII (HCA159) antibody

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