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IgM antibody | MARM-4

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Mouse anti Rat IgM Heavy Chain:FITC

Product Type
Monoclonal Antibody
Clone
MARM-4
Isotype
IgG1
Specificity
IgM
Region
HEAVY CHAIN

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Product Code Applications Pack Size List Price Your Price Qty
MCA189F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.5 mg loader
List Price Your Price
loader

Mouse anti Rat IgM Heavy Chain antibody, clone MARM-4 recognizes the mu heavy chain of rat immunoglobulin and does not cross-react with other immunoglobulin classes.

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Immunogen
IR202 rat IgM myeloma protein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 10ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for IgM antibody

  1. Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
    Eur J Immunol. 4 (1): 44-8.
  2. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
    J Immunol. 121 (5): 2077-82.
  3. Khalifeh, M.S. et al. (2010) Investigation ofthe role of tumour necrosis factor-{alpha}, interleukin-1{beta}, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis.
    Lab Anim. 44: 143-9.
  4. Bézie, S. et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
    J Immunol. 195 (12): 5805-15.
  5. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
    Int Immunol. 30 (2): 53-67.
  6. Tulinská, J. et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
    Arch Toxicol. 92 (7): 2385-99.

RRID
AB_321876

MCA189F

149747 154402 161139 166087

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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