Flow Cytometry Explained
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Flow cytometry is a powerful tool for measuring the properties of single cells or particles and has a wide range of applications in research and diagnostics. The basic principle is to pass cells through a narrow fluidic stream, illuminating individual cells with a laser light as they pass through. The pattern of how light is scattered after it hits each cell reveals cellular properties such as size, shape, viability, and the presence of fluorescently-tagged proteins of interest.
In this section, we have brought together our collective knowledge and experience for you to utilize so that you can achieve success in your own experiments. From educational articles and protocols to webinars, posters, and essential tools, our Flow Cytometry Explained Knowledge Hub is the only place you need to visit to find your essential flow cytometry resources.
As well as the comprehensive set of resources here, which includes the essential Introduction to Flow Cytometry Guide, Bio-Rad is on hand with technical support for any queries requiring an extra level of expertise.
Flow Cytometry Knowledge
Articles
- Antibody titration
- Antigen density
- Cancer research with flow cytometry
- Car T-Cell development with flow cytometry
- Cell frequencies in common samples
- Controls in flow cytometry
- Exosome detection
- Flow cytometry applications
- Fluorophores
- Gating strategies
- High-throughput screening for flow cytometry
- How a flow cytometer works
- Identification of B cells
- Identification of myeloid cells
- Immunophenotyping by flow cytometry
- Immune aging and senescence
- Intracellular flow cytometry
- Premixing StarBright Dyes
- Stain index
- StarBright Dyes in premixed cocktails
- Using compensation beads with StarBright Dyes
Webinars
- Dead or alive on the ZE5 - Using flow cytometry to measure apoptosis
- Flow cytometry and our understanding of COVID-19
- Flow cytometry shorts - Compensation controls
- Flow cytometry shorts - Isotype controls
- Flow cytometry shorts - Other flow controls
- Flow cytometry shorts - Viability controls
- Flow cytometry, the ZE5 Cell Analyzer, and the fight against COVID-19
- Fluorescence and compensation in flow cytometry
- Multicolor panel building in flow cytometry
- Optimize your flow cytometry
- Take control of your flow cytometry
- The latest in flow cytometry – The rise of the 488 nm Laser
- To boldly flow
- StarBright Dye webinars
Featured Blogs
- 5 easy steps for successful flow cytometry
- A guide to gating in flow cytometry
- How to get more out of your multicolor panels
- Intracellular flow cytometry - The good, the bad, and the cytokines
- Introduction to spectral flow cytometry
- Set yourself up for success with flow cytometry
- Tandem dyes - The power couples of flow cytometry
Tips, Guidance and Support
Tools
- Build multicolor flow cytometry panels
- Flow cytometry design tool
- Flow cytometry experiment planning
- Fluorescent spectraviewer
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Protocols
Technical Notes
Podcasts - Spin Break
Flow Cytometry Resources
Downloadable Guides
Posters
Antibody Performance Guarantee
Bio-Rad offers a performance guarantee in key immunological applications including flow cytometry, immunohistochemistry, immunoassays, western blotting, and immunofluorescence.
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