List Price Quantity
ICT960
TBC

Caspase-9

  • Product Code
    ICT960
  • Description
    SR FLICA™ Caspase-9 Kit
  • Product Type
    Kits
  • Format
    Kit
  • Applications
    F, IF
  • Pack Size
    25 Tests
Product Summary
Additional Formats
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Application Notes
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Product Information

The SR FLICA Caspase 9 kit uses a quick and easy method to analyze active caspases in apoptotic cells.

Reagents In The Kit

1 vial of SR-LEHD-FMK FLICA Reagent - lyophilized
10X Wash Buffer, 15 mL
Fixative, 6 mL
Hoechst Stain, 1 mL

Test Principle

Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a red (Sulforhodamine, SR) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Storage

Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the FLICA reagent from light at all times.

Once reconstituted, the 150X FLICA stock should be stored at -20°C protected from light.

Shelf Life

The 150X FLICA reconstituted stock is stable for up to 6 months and may be thawed twice during that time.

Useful Reagents

FormatProduct CodePack SizeApplicationsList PriceQuantity
KitICT961100 TestsF, IF
ICT961

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Application

Application NameYesNoNot DeterminedSuggested Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use

Instructions for use can be found at www.abdserotec.com/uploads/IFU/ICT960.pdf

Acknowledgements

FLICA is a trademark of Immunochemistry Technologies, LLC.

Regulatory

For research purposes only
Copyright © 2013 Bio-Rad AbD Serotec
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