The SR FLICA™ Caspase 9 kit uses a quick and easy method to analyze active caspases in apoptotic cells.
Reagents In The Kit
1 vial of SR-LEHD-FMK FLICA™ Reagent - lyophilized
10X Wash Buffer, 15 mL
Fixative, 6 mL
Hoechst Stain, 1 mL
Caspase FLICA™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA™ reagent contains a caspase inhibitor sequence linked to a red (Sulforhodamine, SR) fluorescent probe.
The Caspase FLICA™ Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.
This kit can be used with a fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the FLICA™ reagent from light at all times.
Once reconstituted, the 150X FLICA™ stock should be stored at -20°C protected from light.
The 150X FLICA™ reconstituted stock is stable for up to 6 months and may be thawed twice during that time.