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MCA1346F
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CD4 Antibody

  • Product Code
    MCA1346F
  • Description
    Mouse anti Cat CD4:FITC
  • Product Type
    Monoclonal Antibody
  • Clone
    vpg34
  • Isotype
    IgG1
  • Format
    FITC
  • Applications
    F
  • Pack Size
    0.1 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

CD4 Antibody Product Summary

Specificity

Mouse anti Cat CD4 antibody, clone vpg34 recognizes the feline homolog of the human CD4 antigen. CD4 is not expressed on feline monocytes.

Target Species

Cat

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preservative Stabilisers

0.09%Sodium Azide
1%Bovine Serum Albumin

Immunogen

Immunoaffinity purified feline CD4

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Buffer Solution

Phosphate buffered saline

Fusion Partners

Spleen cells from immunised BALB/c were fused with cells of the NSO mouse myeloma cell line

Storage

Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

CD4 Antibody Additional Formats

CD4 Antibody Related Reagents

CD4 Antibody More Images

  • Application NameReference Images
    Flow Cytometry

CD4 Antibody Application Notes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
Application NameYesNoNot DeterminedSuggested Dilution
Flow CytometryNeat - 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul

CD4 Antibody References

References

1. Willett, B.J. et al. (1994) The generation of monoclonal antibodies recognising novel epitopes by immunisation with solid matrix antigen-antibody complexes reveals a polymorphic determinant on feline CD4.
J. Immunol. Methods. 176: 213-220.
2. Campbell, D.J. et al. (2004) Age-related differences in parameters of feline immune status.
Vet Immunol Immunopathol. 100: 73-80.
3. Campbell, D.J. et al. (2004) Insulin-like growth factor-I (IGF-I) and its association with lymphocyte homeostasis in the ageing cat.
Mech Ageing Dev. 125: 497-505.
4. Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in cats.
Vet Ther. 8: 229-38.
5. Freer, G. et al. (2008) Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course.
Retrovirology. 5: 33.
6. Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
J Virol. 84: 3845-56.
7. Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
J Virol. 81: 9665-79.
8. Reinero, C.R. et al. (2008) Adjuvanted rush immunotherapy using CpG oligodeoxynucleotides in experimental feline allergic asthma.
Vet Immunol Immunopathol. 121: 241-50.
9. Carreño, A.D. et al. (2008) Loss of naïve (CD45RA+) CD4+ lymphocytes during pediatric infection with feline immunodeficiency virus.
Vet Immunol Immunopathol. 121: 161-8.
10. Flynn, J.N. et al. (2002) Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection.
J Virol. 76: 2306-15.
11. Hosie, M.J. et al. (2000) Vaccination with inactivated virus but not viral DNA reduces virus load following challenge with a heterologous and virulent isolate of feline immunodeficiency virus.
J Virol. 74: 9403-11.
12. Hosie, M.J. et al. (2002) Evolution of replication efficiency following infection with a molecularly cloned feline immunodeficiency virus of low virulence.
J Virol. 76: 6062-72.
13. Kraase, M. et al. (2010) Feline immunodeficiency virus env gene evolution in experimentally infected cats.
Vet Immunol Immunopathol. 134: 96-106.
14. Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
J S Afr Vet Assoc. 75: 103-7.
15. Novacco, M. et al. (2012) Protection from reinfection in "Candidatus Mycoplasma turicensis"-infected cats and characterization of the immune response.
Vet Res. 43: 82.
16. Reche, A.Jr. et al. (2010) Cutaneous mycoflora and CD4:CD8 ratio of cats infected with feline immunodeficiency virus.
J Feline Med Surg. 12: 355-8.
17. Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
PLoS One. 8: e54871.

CD4 Antibody More Information

Health And Safety Information

Material Safety Datasheet Documentation #10041 available at:
http://www.abdserotec.com/uploads/MSDS/10041.pdf

Regulatory

For research purposes only
Copyright © 2014 Bio-Rad AbD Serotec

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