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CD140b antibody | PR7212

Product Code Applications Pack Size List Price Your Price Qty
7460-3104
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP WB 0.1 mg loader
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loader

Mouse anti Human CD140b antibody, clone PR7212 recognizes the extracellular domain of the human PDGF receptor beta subunit., confirmed using several different cell lines and by its ability to immunoprecipitate PDGF receptor beta complexed with 125I-PDGF-BB (Hart et al. 1987). Mouse anti Human CD140b antibody, clone PR7212 does not recognize PDGF receptor alpha.

PDGF receptor beta is a member of the class III receptor tyrosine kinase family that also includes M-CSF receptor, SCF receptor and Flt-3. Binding of PDGF-BB induces receptor homodimerization or heterodimerization with PDGF receptor alpha.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Baboon
Rat
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human skin fibroblast cell membrane extracts.
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 25 �g/ml
Immunofluorescence
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Human breast cancer tissue

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

Source Reference

  1. Hart, C.E. et al. (1987) Synthesis, phosphorylation, and degradation of multiple forms of the platelet-derived growth factor receptor studied using a monoclonal antibody.
    J Biol Chem. 262: 10780-5.

References for CD140b antibody

  1. Hart, C.E. et al. (1988) Two classes of PDGF receptor recognize different isoforms of PDGF.
    Science. 240: 1529-31.
  2. Seifert, R.A. et al. (1989) Two different subunits associate to create isoform-specific platelet-derived growth factor receptors.
    J Biol Chem. 264: 8771-8.
  3. Franklin, W.A. et al. (1990) In situ distribution of the beta-subunit of platelet-derived growth factor receptor in nonneoplastic tissue and in soft tissue tumors.
    Cancer Res. 50: 6344-8.
  4. Krane, J.F. et al. (1991) Increased dermal expression of platelet-derived growth factor receptors in growth-activated skin wounds and psoriasis.
    J Invest Dermatol. 96: 983-6.
  5. Palman, C. et al. (1992) Platelet-derived growth factor receptor (beta-subunit) immunoreactivity in soft tissue tumors.
    Lab Invest. 66: 108-15.
  6. Wang, J. et al. (1994) Cell proliferation in human soft tissue tumors correlates with platelet-derived growth factor B chain expression: an immunohistochemical and in situ hybridization study.
    Cancer Res. 54: 560-4.
  7. Ascoli, V. et al. (1995) Platelet-derived growth factor receptor immunoreactivity in mesothelioma and nonneoplastic mesothelial cells in serous effusions.
    Acta Cytol. 39: 613-22.
  8. Gilbertson, D.G. et al. (2001) Platelet-derived growth factor C (PDGF-C), a novel growth factor that binds to PDGF alpha and beta receptor.
    J Biol Chem. 276: 27406-14.
  9. View The Latest Product References
  10. Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
    PLoS One. 6: e17454.

Functional Assays

Immunoprecipitation

Synonyms
PDGF Receptor Beta
RRID
AB_2162788
UniProt
P09619
Entrez Gene
PDGFRB
GO Terms
GO:0004992 platelet activating factor receptor activity
GO:0005019 platelet-derived growth factor beta-receptor activity
GO:0005021 vascular endothelial growth factor receptor activity
GO:0005887 integral to plasma membrane
GO:0005161 platelet-derived growth factor receptor binding
GO:0005524 ATP binding
GO:0008284 positive regulation of cell proliferation
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0030335 positive regulation of cell migration
GO:0046777 protein autophosphorylation
GO:0048407 platelet-derived growth factor binding
GO:0060326 cell chemotaxis

7460-3104

152757 159456 165280

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