CD51 antibody | RMV-7
Rat anti Mouse CD51
- Product Type
- Monoclonal Antibody
- Clone
- RMV-7
- Isotype
- IgG1
- Specificity
- CD51
Rat anti Mouse CD51 antibody, clone RMV-7 recognizes murine CD51, a ~140 kDa alpha subunit of the vitronectin receptor, which is otherwise known as the integrin alpha v chain. CD51 can form heterodimers at the cell surface with a variety of beta integrins including CD29 and CD61. Heterodimers of CD51/CD61 function as a receptor for vitronectin, and a wide array of RGD-containing proteins including fibronectin, fibrinogen, von Willebrand factor, laminin, thrombospondin and osteopontin. CD51/CD61 is primarily expressed on myeloid cells and activated T-cells. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing. Rat anti Mouse CD51 antibody, clone RMV-7 has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity (Takahashi et al. 1990). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Cultured LAK cells from Balb/c mice.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized SD rats were fused with the cells of the P3X.63Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/25 Pack Size: 0.25 mg 1/10 Pack Size: 0.1 mg |
1/100 | |
Immunohistology - Frozen | 1/25 | 1/100 | |
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD51 antibody
-
Takahashi, K. et al. (1990) A murine very late activation antigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-target cell interaction.
J Immunol. 145 (12): 4371-9. -
Piali, L. et al. (1995) CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium.
J Cell Biol. 130 (2): 451-60. -
Takahashi F et al. (2001) Role of osteopontin in the pathogenesis of bleomycin-induced pulmonary fibrosis.
Am J Respir Cell Mol Biol. 24 (3): 264-71. -
Cui R et al. (2007) Abrogation of the interaction between osteopontin and alphavbeta3 integrin reduces tumor growth of human lung cancer cells in mice.
Lung Cancer. 57 (3): 302-10.
Further Reading
-
Henderson, N.C. et al. (2013) Targeting of ααv integrin identifies a core molecular pathway that regulates fibrosis in several organs.
Nat Med. 19 (12): 1617-24.
- Synonyms
- Integrin Alpha V
- RRID
- AB_931725
- UniProt
- P43406
- Entrez Gene
- Itgav
- GO Terms
- GO:0001525 angiogenesis
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0030154 cell differentiation
- GO:0043277 apoptotic cell clearance
MCA2461
163600 1709MCA2461GA
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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