Product Images

  • Hamster anti Mouse TCR Gamma/DeltaRPE antibody (MCA1366PE) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (BUF041B)

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List Price Quantity
BUF041B
TBC
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Mouse Seroblock FcR

  • Product Code
    BUF041B
  • Description
    Mouse Seroblock FcR
  • Product Type
    Accessory Reagent
  • Clone
    FCR4G8
  • Isotype
    IgG2b
  • Format
    Reagent
  • Applications
    F
  • Pack Size
    0.5 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

Product Information

BUF041B is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRgammaIII and FcRgammaII, respectively.

The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes.

The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in “high background” staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with BUF041B, resulting in clearer staining.

For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MCA2305F), AlexaFluor488 (MCA2305A488), AlexaFluor647 (MCA2305A647), Biotin (MCA2305B) and RPE (MCA2305PE).

Target Species

Mouse

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preservative Stabilisers

0.09%Sodium Azide (NaN3)

Immunogen

PU5 1.8 IOE7 Balb/c mouse cell line

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Buffer Solution

Phosphate buffered saline

Storage

Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.
  • FormatProduct CodePack SizeApplicationsList PriceQuantity
    ReagentBUF041A0.1 mgF
    BUF041A
    • Product Images

    • Hamster anti Mouse TCR Gamma/DeltaRPE antibody (MCA1366PE) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (BUF041B)

Application

Application NameYesNoNot DeterminedSuggested Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use

In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of BUF041B (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.

2) Add test antibody according to manufacturers instructions – Do not wash BUF041B off the cells. BUF041B is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*

3) Proceed with staining as usual.

* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. AbD Serotec supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose.

References

1. Ivanovska, N.D et al . (2008) Properdin Deficiency in Murine Models of Nonseptic Shock.
The Journal of Immunology. 180: 6962-6969.
2. Chen, H-F et al . (2009) A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals.
Human Reproduction. 24: 71-80.
3. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
J Immunol. 186: 3441-51.

Health And Safety Information

Material Safety Datasheet Documentation #10040 available at:
http://www.abdserotec.com/uploads/MSDS/10040.pdf

Regulatory

For research purposes only
Copyright © 2013 Bio-Rad AbD Serotec
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