BUF041B is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRgammaIII and FcRgammaII, respectively.
The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes.
The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in “high background” staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with BUF041B, resulting in clearer staining.
For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MCA2305F
), AlexaFluor488 (MCA2305A488
), AlexaFluor647 (MCA2305A647
), Biotin (MCA2305B
) and RPE (MCA2305PE
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
PU5 1.8 IOE7 Balb/c mouse cell line
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Phosphate buffered saline
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch.