Proteus: Endotoxin Removal Kits

MIDI Kits

  • Rapid removal of endotoxin in less than an hour
  • Endotoxin binding capacity of 450,000 EU/ml
  • High capacity and standard capacity formats

Proteus Endotoxin Removal MIDI Kits contain pre-packed affinity resin columns with extremely high endotoxin binding capacity, to achieve endotoxin removal effectively and quickly. These high performance kits result in more than 95% Endotoxin Removal in one passage, and greater than 90% Protein Recovery.

Proteus Endotoxin Removal MIDI Kits are available with different endotoxin binding capacities. Either kit can be used for samples with endotoxin loads less than 30,000 EU.  For samples with endotoxin loads less than 1,000,000 EU, high capacity columns are required.

High Capacity or Standard Capacity Columns?

This data shows how the Proteus Low Endotoxin High Capacity Kits can effectively remove endotoxin from Rabbit IgG smaples (1mg/ml), spiked with E.coli lysate

MINI Kits

Proteus Endotoxin Removal MINI Kits are designed for small sample volumes and offer a standardized method for high grade removal of endotoxin from a broad spectrum of monoclonal antibodies and proteins. Proteus Endotoxin Removal MINI Kits incorporate a proprietary membrane technology, preventing any sample leaking into the collection tube or onto an orbital mixer.

 

Simple workflow of Proteus Endotoxin Removal MINI Kits

Product Specifications

Kit MIDI Standard Capacity MIDI High Capacity MINI columns
Typical binding capacity per column 30,000 EU 500,000 - 1,000,000 EU 3,000 EU
Typical endotoxin clearance
2 passes 		4 log reduction 4 log reduction N/A
Typical endotoxin clearance
(3 hour incubation) 		N/A N/A 3 log reduction
Maximum sample load volume 20 ml 20 ml 20 ml
Bead size range 100 µm 100 µm 100 µm
Resin bed volume 1 ml (pre-packed) 1.7 ml (pre-packed) 0.25 ml (loose resin)
Recommended working pH pH 4-8 pH 4-8 pH 4-8
Color coded end caps Light green Dark green Supplied in vials

Endotoxin Removal Kits Range

Description Centrifuge
tubes		 High
capacity		 Quantity  Product Code
Proteus Endotoxin Removal MIDI Spin Column Pack* Yes Yes 12 Units PUR028
Proteus Endotoxin Removal MIDI Spin Column Pack* Yes Yes 12 Units PUR030
Proteus Endotoxin Removal MINI Spin column Pack* Yes No 12 Units PUR032

Abbreviations:  * Spin Column Packs contain the following: spin columns, spin column plugs, centrifuge tubes and plug insertion tools.

Endotoxin Removal Kit FAQs

To answer any questions you may have about Proteus Kits, please consult our Proteus Purification Kit: FAQs Page.

Also in the Proteus Range: